Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement

Stallmann, Hein P.; Faber, Chris; Bronckers, A.L.J.J.; Amerongen, A. van Nieuw; Wuisman, P.

doi:10.1093/jac/dkh346

Cited by 63 publications

(60 citation statements)

References 29 publications

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“…In rabbit model trials using cement containing hLf (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11), two studies [56,57] revealed a significant reduction of bone-injected S. aureus ATCC 10832 bacteria or S. aureus (W234) compared with the control group. The results clearly showed that hLf (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) has the ability to reduce osteomyelitis, with microbiological results similar to those of gentamicin [57].…”

Section: Main In Vivo Results In Human and Animal Modelsmentioning

confidence: 99%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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Antimicrobial peptides (AMPs) represent a vast array of molecules produced by virtually all living organisms as natural barriers against infection. Among AMP sources, an interesting class regards the food-derived bioactive agents. The whey protein lactoferrin (Lf) is an iron-binding glycoprotein that plays a significant role in the innate immune system, and is considered as an important host defense molecule. In search for novel antimicrobial agents, Lf offers a new source with potential pharmaceutical applications. The Lf-derived peptides Lf(1-11), lactoferricin (Lfcin) and lactoferrampin exhibit interesting and more potent antimicrobial actions than intact protein.Particularly, Lfcin has demonstrated strong antibacterial, anti-fungal and antiparasitic activity with promising applications both in human and veterinary diseases (from ocular infections to osteo-articular, gastrointestinal and dermatological diseases).

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Section: Main In Vivo Results In Human and Animal Modelsmentioning

confidence: 99%

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Bruni¹,

et al. 2016

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“…AMPs are also generally able to distinguish between mammalian and bacterial cell membranes due to the predominance of negatively charged lipids on the surface of bacterial membranes not seen with mammalian cells [42]. An example of a naturally occurring antimicrobial peptide is human lactoferrin 1-11 [45]. Synthetic analogues of these naturally-occurring peptides, such as arylamide foldamers, have been synthesized and shown to be effective against bacteria [42,43].…”

Section: Alternatives To Conventional Antibioticsmentioning

confidence: 99%

“…There has been interest in local delivery of AMPs as an alternative to conventional antibiotics. Several studies have described local delivery of AMPs in vitro and in vivo via calcium phosphate carrier [44][45][46]. These studies show that it is possible to incorporate and locally release AMPs while retaining antimicrobial activity, although broad-spectrum AMPs such as human lactoferrin 1-11 may not be as potent as conventional antibiotics such as gentamicin [44][45][46].…”

Section: Alternatives To Conventional Antibioticsmentioning

confidence: 99%

“…Several studies have described local delivery of AMPs in vitro and in vivo via calcium phosphate carrier [44][45][46]. These studies show that it is possible to incorporate and locally release AMPs while retaining antimicrobial activity, although broad-spectrum AMPs such as human lactoferrin 1-11 may not be as potent as conventional antibiotics such as gentamicin [44][45][46]. Shukla et al [47] incorporated a different AMP, ponericin G1, into a degradable polyectrolyte multilayer film that has applications as coatings for medical devices and wound healing.…”

Section: Alternatives To Conventional Antibioticsmentioning

confidence: 99%

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Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

2013

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“…In general calcium phosphate bone cements showed a high and prolonged in vitro release of hLF1-11 in its biologically active form (22). Furthermore, we also showed that hLF1-11 incorporated into calcium phosphate bone cement can prevent S. aureus osteomyelitis in rabbits (21). Therefore, the aim of the present investigation was to evaluate and compare the performance of hLF1-11 and gentamicin incorporated into calcium phosphate bone cement to treat MRSA osteomyelitis in rabbits.…”

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confidence: 90%

Comparable Efficacies of the Antimicrobial Peptide Human Lactoferrin 1-11 and Gentamicin in a Chronic Methicillin-Resistant Staphylococcus aureus Osteomyelitis Model

Faber

Stallmann

Lyaruu

et al. 2005

Antimicrob Agents Chemother

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The therapeutic efficacy of an antimicrobial peptide, human lactoferrin 1-11 (hLF1-11), was investigated in a model of chronic methicillin-resistant Staphylococcus aureus (MRSA) (gentamicin susceptible) osteomyelitis in rabbits. We incorporated 50 mg hLF1-11/g or 50 mg gentamicin/g cement powder into a calcium phosphate bone cement (Ca-P) and injected it into the debrided tibial cavity, creating a local drug delivery system. The efficacy of hLF1-11 and gentamicin was compared to that of a sham-treated control (plain bone cement) (n ‫؍‬ 6) and no treatment (infected only) (n ‫؍‬ 5). The results were evaluated by microbiology, radiology, and histology. MRSA was recovered from all tibias in both control groups (n ‫؍‬ 11). On the other hand, hLF1-11 and gentamicin significantly reduced the bacterial load. Furthermore, no growth of bacteria was detected in five out of eight and six out of eight specimens of the hLF1-11-and gentamicin-treated groups, respectively. These results were confirmed by a significant reduction of the histological disease severity score by hLF1-11 and gentamicin compared to both control groups. The hLF1-11-treated group also had a significantly lower radiological score compared to the gentamicin-treated group. This study demonstrates the efficacy of hLF1-11 incorporated into Ca-P bone cement as a possible therapeutic strategy for the treatment of osteomyelitis, showing efficacy comparable to that of gentamicin. Therefore, the results of this study warrant further preclinical investigations into the possibilities of using hLF1-11 for the treatment of osteomyelitis.

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Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement

Abstract: Local prophylaxis with hLF1-11 effectively reduced development of osteomyelitis in a rabbit model, but gentamicin resulted in a larger number of sterile femora.

Cited by 63 publications

References 29 publications

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

Comparable Efficacies of the Antimicrobial Peptide Human Lactoferrin 1-11 and Gentamicin in a Chronic Methicillin-Resistant Staphylococcus aureus Osteomyelitis Model

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