Osteoinductive porous biphasic calcium phosphate ceramic as an alternative to autogenous bone grafting in the treatment of mandibular bone critical‐size defects

Abstract

The bone-induction capacity of a porous biphasic calcium phosphate (pBCP) using heterotopic implantation in mouse (mHI-model) and its efficacy as substitute for autograft in mandibular critical-size defect in rabbit (rabMCSD-model) was investigated. In mHI-model, pBCP was implanted into the thigh muscles and bone formation was histomorphometrically and immunohistochemically evaluated. In rabMCSD-model, 13 mm bone defects were treated with pBCP or autograft and bone repair comparatively evaluated by radiographic and histomorphometric methods. In mHI-model, formed bone and immunolabeling for bone morphogenetic protein-2 and osteopontin were observed in 90% of pBCP implanted samples after 12 weeks. In rabMCSD-model neither statistically significant difference was found in newly formed bone between pBCP and autograft groups at 4 weeks (18.8 ± 5.5% vs 27.1 ± 5.6%), 8 weeks (22.3 ± 2.7% vs 26.2 ± 5.1), and 12 weeks (19.6 ± 4.7% vs 19.6 ± 2.3%). At 12 weeks, the stability and contour of the mandible were restored in both treatments. Near tooth remaining, pBCP particles were covered by small amount of mineralized tissue exhibiting perpendicular attachments of collagen fiber bundles with histological characteristic of acellular cementum. Within the limitations of this study, it was concluded that pBCP is osteoinductive and able to stimulate the new formation of bone and cementum-like tissues in rabMCSD-model, suggesting that it may be an alternative to treatment of large bone defect and in periodontal regenerative therapy. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1546-1557, 2018.

Bone formation following sinus grafting with an alloplastic biphasic calcium phosphate in Lanyu Taiwanese mini‐pigs

“…37 The most commonly used calcium phosphate ceramics are HA and -TCP at various weight ratios. 41,42 The high biocompatibility, increase safety, high predictability, unlimited availability, low morbidity for patients, and cost-effectiveness of calcium phosphate ceramics all represent important advantages over autografts and allografts. 41,42 The high biocompatibility, increase safety, high predictability, unlimited availability, low morbidity for patients, and cost-effectiveness of calcium phosphate ceramics all represent important advantages over autografts and allografts.…”

“…[38][39][40] Calcium phosphate ceramics have shown bioactivity and osteoconductivity in previous papers. 41,42 The high biocompatibility, increase safety, high predictability, unlimited availability, low morbidity for patients, and cost-effectiveness of calcium phosphate ceramics all represent important advantages over autografts and allografts. 43 Although the ideal ratio of HA to -TCP is still under investigation, [44][45][46] variant ratios of BCPs have been widely used for bone graft substitutes, with favorable results A review of the commercially available BCP indicated that the most common ratio is 60% HA and 40% -TCP.…”

Reconstruction of Craniomaxillofacial Bone Defects Using Tissue-Engineering Strategies with Injectable and Non-Injectable Scaffolds

“…The biomaterials used for scaffold preparation are natural biopolymers, synthetic polymers, ceramics and composites [ 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ]. Natural biopolymers include chitosan [ 28 , 60 , 61 , 62 , 63 ], alginate [ 46 , 64 , 65 ], cellulose [ 66 , 67 , 68 ], collagen [ 33 , 35 , 69 ], hyaluronan [ 70 , 71 , 72 ], fibrin [ 73 , 74 , 75 , 76 ] and silk [ 77 , 78 ].…”

Pre-clinical and clinical studies of a novel porous biphasic calcium phosphate ceramic as alternative to repair bone defects

“…Thus, bone formation occurred around the BCP granule surfaces locally, but in a panoramic view, the bone was formed from the border toward the center of the defects and also from the duramater side toward the teguments (figure 5, 12 st article). A previous study from our research group analyzed HA/TCP 70/30 implanted in mandibular critical size defects and within muscle bundles and reported new bone formation over their surface, pores and concavities (Santos et al, 2018). While comparing granules size of this BCP, different microenvironments were stablished that may explain the differences in bone formation rate among the granular size groups.…”