Reconstruction of Craniomaxillofacial Bone Defects Using Tissue-Engineering Strategies with Injectable and Non-Injectable Scaffolds

Gaihre, Bipin; Uswatta, Suren; Jayasuriya, Ambalangodage C.

doi:10.3390/jfb8040049

Cited by 53 publications

(46 citation statements)

References 122 publications

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“…Engineering the maxillofacial bones is challenging due to the presence of complex physiological structures such as sensory organs, facial skeletal features, cartilage and blood vessels. Moreover, clinicians have to control bacterial contamination in highly susceptible areas, including the oral and nasal regions 16 .…”

Section: Discussionmentioning

confidence: 99%

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Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst

Fliefel

Burian

et al. 2020

Sci Rep

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Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADScs) with tri-calcium phosphates (tcp) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixteen skeletally mature miniature pigs were divided into two groups: Control group and scaffolds seeded with osteogenic differentiated pADSCs (n = 8/group). TCP-PLGA scaffolds with or without cells were integrated in the mandibular critical size defects and fixed by titanium osteosynthesis plates. After 12 weeks, ADSCs seeded scaffolds (n = 7) demonstrated significantly higher bone volume (34.8% ± 4.80%) than scaffolds implanted without cells (n = 6, 22.4% ± 9.85%) in the micro-CT (p < 0.05). Moreover, an increased amount of osteocalcin deposition was found in the test group in comparison to the control group (27.98 ± 2.81% vs 17.10 ± 3.57%, p < 0.001). In conclusion, ADSCs seeding on ceramic/polymer scaffolds improves bone regeneration in large mandibular defects. However, further improvement with regard to the osteogenic capacity is necessary to transfer this concept into clinical use. Maxillofacial bone defects, which occur due to trauma, craniofacial deformities, tumour or infection can lead to facial deformities and severe maxillofacial dysfunctions provoking a dramatic decrease in the quality of life of the patients 1,2. The reconstruction of large bone defects poses many challenges in oral and maxillofacial surgery. Although autologous bone grafts are considered the gold standard in bone defect repair, there are some concerns related to the limited supply and donor site morbidity 3. Several alternatives as allografts, xenografts or synthetic bone substitutes have been brought by researchers and clinicians to restore the function and architecture of the defective bone but still cannot solve the problem due to various limitations. The search of new treatment alternatives has emerged enormously in the past few

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Section: Discussionmentioning

confidence: 99%

“…The regeneration of facial skeletal tissues must consider ways to ensure the restoration of aesthetics. Additionally, reconstruction should give sufficient mechanical strength and support movement due to speech and masticatory functions 16 .…”

Section: Discussionmentioning

confidence: 99%

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst

Fliefel

Burian

et al. 2020

Sci Rep

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“…The success of this approach has resulted in a range of popular, commercially available, hydroxyapatite powders, cements, and granules including: Bio‐Oss (Geistlich Ltd., Switzerland) and Fisiograft Bone (Ghimas S.p.A, Italy). In addition, hybridization of inorganic materials with polymers and/or growth factors has been explored as injectable composite materials for craniomaxillofacial bone tissue engineering 12. Similarly, membranes made from synthetic ( exe .…”

Section: Introductionmentioning

confidence: 99%

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

Okesola

Derkuş

et al. 2020

Adv Funct Materials

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Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system that integrates hyaluronic acid tyramine (HA‐Tyr), bioactive peptide amphiphiles (GHK‐Cu2+), and Laponite (Lap) to engineer hydrogels with physical, mechanical, and biomolecular signals that can be tuned to enhance bone regeneration is reported. The central design element of the multicomponent hydrogels is the integration of self‐assembly and enzyme‐mediated oxidative coupling to optimize structure and mechanical properties in combination with the incorporation of an osteo‐ and angio‐promoting segments to facilitate signaling. Spectroscopic techniques are used to confirm the interplay of orthogonal covalent and supramolecular interactions in multicomponent hydrogel formation. Furthermore, physico‐mechanical characterizations reveal that the multicomponent hydrogels exhibit improved compressive strength, stress relaxation profile, low swelling ratio, and retarded enzymatic degradation compared to the single component hydrogels. Applicability is validated in vitro using human mesenchymal stem cells and human umbilical vein endothelial cells, and in vivo using a rabbit maxillary sinus floor reconstruction model. Animals treated with the HA‐Tyr‐HA‐Tyr‐GHK‐Cu2+ hydrogels exhibit significantly enhanced bone formation relative to controls including the commercially available Bio‐Oss.

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“…Popular stem cell used in dental tissue engineering is the periodontal ligament stem cells (PDLSC) which is extracted from discarded teeth and has the potential to generate the cementum and periodontal ligament-like structure. Studies have shown that these stem cells also have the potential to develop into the osteogenic and adipogenic tissues in vitro, opening up multiple opportunities for tissue engineering from dental-derived stem cells [46].…”

Section: Stem Cells In the Scaffoldsmentioning

confidence: 99%

Prospectives for using artificial scaffolds in oral and craniofacial surgery: literature review

Yaremenko¹,

Lysenko²,

Ivanova³

et al. 2018

CTT

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Regenerative medicine is an emerging field of biotechnology that combines various aspects of medicine including cell and molecular biology, material science and bioengineering -to regenerate, repair or replace tissues. Bone regeneration is a promising approach in dentistry and is considered an ideal clinical strategy in treating diseases, injuries, and defects of the maxillofacial area. Advances in tissue engineering have resulted in the development of innovative scaffold designs, complemented by the progress made in cell-based therapies. In vitro bone regeneration can be achieved by the combination of stem cells, scaffolds, and bioactive factors. A possible improvement in restoring damaged tissues may be achieved by load-ing the scaffolds with drug substances, as well as genetic material, growth factors or other proteins, promoting the tissue regeneration. This review focuses on different biomaterials currently used in dentistry, as potential scaffolds for bone regeneration when treating bone defects, or in surgical interventions, including characteristics and types of these scaffolds, and a literature review of local antibiotic delivery by combined usage of scaffolds and drug-delivery systems.

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Reconstruction of Craniomaxillofacial Bone Defects Using Tissue-Engineering Strategies with Injectable and Non-Injectable Scaffolds

Cited by 53 publications

References 122 publications

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

Prospectives for using artificial scaffolds in oral and craniofacial surgery: literature review

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