Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst, Florian; Fliefel, Riham; Burian, Egon; Probst, Monika; Eddicks, Matthias; Cornelsen, Matthias; Riedl, Christina; Seitz, Hermann; Aszódi, Attila; Schieker, Matthias; Otto, Sven

doi:10.1038/s41598-020-59038-8

Cited by 58 publications

(63 citation statements)

References 69 publications

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“…There is no real consensus on the critical size, it varied from 2 to 10 cm 3 depending on the location (tooth bearing area or not, full thickness or not, anterior or posterior area, periosteum preservation or not…) [31]. Otto and coworkers recently proposed a 6 cm 3 mandibular defect [32]. We carried out a full thickness defect on the basilar border of the mandibular angle.…”

Section: Discussionmentioning

confidence: 99%

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Bouyer

Garot

Machillot

et al. 2020

Preprint

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Abstractthe reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed a new critical-size mandibular bone defect model on a mini-pig, close to human clinical issues. We analyzed the bone reconstruction obtained by a 3D printed scaffold made of clinical-grade PLA, coated with a polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). We compared the results (CT-scan, μCT, histology) to the gold standard solution, bone autograft. We demonstrated that the dose of BMP-2 delivered from the scaffold significantly influenced the amount of regenerated bone and the repair kinetics, with a clear BMP-2 dose-dependence. Bone was homogeneously formed inside the scaffold without ectopic bone formation. The bone repair was as good as for the bone autograft. The BMP-2 doses applied in our study were reduced 20 to 75-fold compared to the commercial collagen sponges used in the current clinical applications, without any adverse effects. 3D printed PLA scaffolds loaded with reduced doses of BMP-2 can be a safe and simple solution for large bone defects faced in the clinic.

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Section: Discussionmentioning

confidence: 99%

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Bouyer

Garot

Machillot

et al. 2020

Preprint

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“…In bone tissue engineering, bone marrow has so far probably gained the greatest attention as a source of MSCs, but due to the invasive and painful procedure of bone marrow aspirate collection which can also cause donor site morbidity, other adult and fetal tissue sources have been studied as the source of MSCs [54]. For instance, several studies have indicated the osteogenic differentiation and bone formation potential of adipose-derived MSCs, which can be isolated from the tissue obtained during liposuction, lipoplasty, or lipectomy procedures with less discomfort and complications compared with bone marrow aspirate collection [55,56]. Further, MSCs derived from umbilical cord blood and peripheral blood with less invasive cell collection methods have also shown their potential for bone defect repair [57][58][59].…”

Section: Cells In Development Of In Vitro Bone Modelsmentioning

confidence: 99%

Cell Sources for Human In vitro Bone Models

Ansari

Ito

Hofmann

2021

Curr Osteoporos Rep

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Purpose of Review One aim in bone tissue engineering is to develop human cell-based, 3D in vitro bone models to study bone physiology and pathology. Due to the heterogeneity of cells among patients, patient’s own cells are needed to be obtained, ideally, from one single cell source. This review attempts to identify the appropriate cell sources for development of such models. Recent Findings Bone marrow and peripheral blood are considered as suitable sources for extraction of osteoblast/osteocyte and osteoclast progenitor cells. Recent studies on these cell sources have shown no significant differences between isolated progenitor cells. However, various parameters such as medium composition affect the cell’s proliferation and differentiation potential which could make the peripheral blood-derived stem cells superior to the ones from bone marrow. Summary Peripheral blood can be considered a suitable source for osteoblast/osteocyte and osteoclast progenitor cells, being less invasive for the patient. However, more investigations are needed focusing on extraction and differentiation of both cell types from the same donor sample of peripheral blood.

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“…In order to overcome the above shortcomings in BTE, PLGA often combines with other biomaterials, such as ceramics like bioglass, HA, TCP and natural polymers (Boukari et al, 2017 ; Zhao et al, 2017 ; Bharadwaz and Jayasuriya, 2020 ; Probst et al, 2020 ). For example, PLGA/HA composite fibrous scaffolds were fabricated by the melt-spinning method, in which BMP-2 was incorporated with the help of polydopamine (Zhao et al, 2017 ).…”

Section: Synthetic Polymers: Artificially Synthesized Organic Biomatementioning

confidence: 99%

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

Zhang

Zhao

et al. 2020

Front. Bioeng. Biotechnol.

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Bone is a dynamic organ with high regenerative potential and provides essential biological functions in the body, such as providing body mobility and protection of internal organs, regulating hematopoietic cell homeostasis, and serving as important mineral reservoir. Bone defects, which can be caused by trauma, cancer and bone disorders, pose formidable public health burdens. Even though autologous bone grafts, allografts, or xenografts have been used clinically, repairing large bone defects remains as a significant clinical challenge. Bone tissue engineering (BTE) emerged as a promising solution to overcome the limitations of autografts and allografts. Ideal bone tissue engineering is to induce bone regeneration through the synergistic integration of biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Successful stem cell-based BTE requires a combination of abundant mesenchymal progenitors with osteogenic potential, suitable biofactors to drive osteogenic differentiation, and cell-friendly scaffold biomaterials. Thus, the crux of BTE lies within the use of cell-friendly biomaterials as scaffolds to overcome extensive bone defects. In this review, we focus on the biocompatibility and cell-friendly features of commonly used scaffold materials, including inorganic compound-based ceramics, natural polymers, synthetic polymers, decellularized extracellular matrix, and in many cases, composite scaffolds using the above existing biomaterials. It is conceivable that combinations of bioactive materials, progenitor cells, growth factors, functionalization techniques, and biomimetic scaffold designs, along with 3D bioprinting technology, will unleash a new era of complex BTE scaffolds tailored to patient-specific applications.

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Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Cited by 58 publications

References 69 publications

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

Cell Sources for Human In vitro Bone Models

Stem Cell-Friendly Scaffold Biomaterials: Applications for Bone Tissue Engineering and Regenerative Medicine

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