Osteogenic Responses to Zirconia with Hydroxyapatite Coating by Aerosol Deposition

Abstract

Previously, we found that osteogenic responses to zirconia co-doped with niobium oxide (Nb ) are comparable with responses to titanium, which is widely used as a dental implant material. The present study aimed to evaluate the in vitro osteogenic potential of hydroxyapatite (HA)-coated zirconia by an aerosol deposition method for improved osseointegration. Surface analysis by scanning electron microscopy and x-ray diffraction proved that a thin as-deposited HA film on zirconia showed a shallow, regular, crater-like surface. Deposition of dense and uniform HA films was measured by SEM, and the contact angle test demonstrated improved wettability of the HA-coated surface. Confocal laser scanning microscopy indicated that MC3T3-E1 pre-osteoblast attachment did not differ notably between the titanium and zirconia surfaces; however, cells on the HA-coated zirconia exhibited a lower proliferation than those on the uncoated zirconia late in the culture. Nevertheless, ALP, alizarin red S staining, and bone marker gene expression analysis indicated good osteogenic responses on HA-coated zirconia. Our results suggest that HA-coating by aerosol deposition improves the quality of surface modification and is favorable to osteogenesis.

Biofunctionalization of zirconia with cell-adhesion peptides via polydopamine crosslinking for soft tissue engineering: effects on the biological behaviors of human gingival fibroblasts and oral bacteria

“…9 However, because it is bioinert and has low reactivity, the use of zirconia is challenging when it comes to fast integration with the surrounding tissue. 10 To address this issue, several surface modication approaches have been proposed to improve the biological performance of zirconia in terms of tissue responses: (i) modifying the surface topography by grinding, sand blasting, 11 or etching; 12 (ii) application of ultraviolet irradiation, 13 plasma spraying, 14 or laser treatment; 15 and (iii) coating of the surface with bioactive agents such as hydroxyapatite, 16 calcium phosphate, 17 chitosan, 18 type I collagen, 19 and bone morphogenetic protein 2. 20 Among these techniques, the physical methods require specialized equipment and conditions, while bioactive coatings generally require complicated procedures and frequently demonstrate poor adhesion to the material.…”

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

“…In a previous study, the LTD phenomenon in zirconia was substantially reduced by addition of niobium oxide (Nb 2 O 5 ) [5,6] or tantalum oxide (Ta 2 O 5 ) [7]. Our previous reports also proved that LTD reinforced tetragonal zirconia polycrystal (TZP) discs containing yttria (Y 2 O 3 ) and niobium oxide (Nb 2 O 5 ) ((Y,Nb)-TZP) have osteogenic potential similar to that of Ti [8], and that the new aerosol deposition technique for hydroxyapatite (HA) coating is significantly effective in enhancing the osteogenic potential [9]. …”

Fibronectin‐Derived Oligopeptide Stimulates Osteoblast Differentiation Through a Bone Morphogenic Protein 2–Like Signaling Pathway

“…A reagent kit ## was used for reverse transcription. Quantitative real‐time PCR was performed for Dlx5, Runx2 , Alp , and Oc genes with primer pairs used previously 22 , 23 . Quantitative real‐time PCR was performed using a real time PCR master mix*** and a real‐time PCR system ††† .…”

“…Quantitative real-time PCR was performed for Dlx5, Runx2, Alp, and Oc genes with primer pairs used previously. 22,23 Quantitative real-time PCR was performed using a real time PCR master mix*** and a real-time PCR system. † † † All experiments were run in triplicate and the relative levels of mRNA were normalized to those of GAPDH.…”