Osteogenic properties of adhesive cells from mouse bone marrow Dexter cultures

Kuznetsov, Sergei A.; Grosheva, A. G.; Fridenshtein, A. Ya.

doi:10.1007/bf00840673

Cited by 9 publications

(6 citation statements)

References 4 publications

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“…In contrast to mBMSC FGF2 , we detected no bone formation in gels containing mBMSC expanded in control medium. Comparison of previous studies using mBMSC ectopically implanted in a collagen sponge, gelatin sponge, or carrier‐free system reveals large differences in the amount of bone obtained, ranging from the absence of bone formation or the presence of only very small bone spicules to the formation of large bone ossicles . This difference may be explained by the percentage of serum that was used during cell expansion, or the growth factor composition and, as our current results suggest, the FGF2 concentration of the serum batch.…”

Section: Discussionsupporting

confidence: 47%

Expansion of Murine Periosteal Progenitor Cells with Fibroblast Growth Factor 2 Reveals an Intrinsic Endochondral Ossification Program Mediated by Bone Morphogenetic Protein 2

et al. 2014

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The preservation of the bone-forming potential of skeletal progenitor cells during their ex vivo expansion remains one of the major challenges for cell-based bone regeneration strategies. We report that expansion of murine periosteal cells in the presence of FGF2, a signal present during the early stages of fracture healing, is necessary and sufficient to maintain their ability to organize in vivo into a cartilage template which gives rise to mature bone. Implantation of FGF2-primed cells in a large bone defect in mice resulted in complete healing, demonstrating the feasibility of using this approach for bone tissue engineering purposes. Mechanistically, the enhanced endochondral ossification potential of FGF2-expanded periosteal cells is predominantly driven by an increased production of BMP2 and is additionally linked to an improved preservation of skeletal progenitor cells in the cultures. This characteristic is unique for periosteal cells, as FGF2-primed bone marrow stromal cells formed significantly less bone and progressed exclusively through the intramembranous pathway, revealing essential differences between both cell pools. Taken together, our findings provide insight in the molecular regulation of fracture repair by identifying a unique interaction between periosteal cells and FGF2. These insights may promote the development of cell-based therapeutic strategies for bone regeneration which are independent of the in vivo use of growth factors, thus limiting undesired side effects.

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Section: Discussionsupporting

confidence: 47%

Expansion of Murine Periosteal Progenitor Cells with Fibroblast Growth Factor 2 Reveals an Intrinsic Endochondral Ossification Program Mediated by Bone Morphogenetic Protein 2

et al. 2014

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“…Previously, Kuznetsov et al (1989) transplanted BMSCs under the renal capsule in mice and noted bone formation within the transplants. They characterized the bone as lamellar, with long trabeculae and abundant hematopoiesis.…”

Section: Discussionmentioning

confidence: 99%

“…Cultured human bone marrow stromal cells (BMSCs) synthesize collagenous and noncollagenous proteins in vitro that are components of normal skeletal matrix (Benayahu et al, 1989;. Following their expansion in tissue culture, the BMSCs from many species are capable of forming new bone when transplanted into immunocompromised recipient mice (Ashton et al, 1980(Ashton et al, , 1984Friedenstein, 1973;Friedenstein et al, 1974;Gundle et al, 1995;Haynesworth et al, 1992;Krebsbach et al, 1997Krebsbach et al, , 1999Kuznetsov et al, 1989;Ohgushi et al, 1990Ohgushi et al, , 1996Thomson et al, 1993). In addition to heterotopic transplantation, BMSCs have been shown to repair induced bone defects in various animal models (Bruder et al, 1998a,b;Casabona et al, 1998;Krebsbach et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape

Mankani

Kuznetsov

Fowler

et al. 2000

Biotechnol. Bioeng.

181

137

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“…Ossicles containing bone marrow were developed in most transplants by 4-5 weeks, and they were maintained for at least five months Friedenstein et al, 1982;Kuznetsov et al, 1989). When mouse BMSCs were transplanted subcutaneously into immunocompromised recipients, they formed bone complete with hematopoietic tissue in all vehicles tested: collagen sponges, collagen matrices, polyvinyl sponges, HA/TCP blocks, and HA/TCP powder (Krebsbach et al, 1997).…”

Section: (A) Transplantation Of Non-human Cells In Open Systemsmentioning

confidence: 99%

Bone Marrow Stromal Cells: Characterization and Clinical Application

Krebsbach

Kuznetsov

Bianco

et al. 1999

Critical Reviews in Oral Biology & Medicine

241

198

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and 3Dipartimento di Medicina Sperimentale, Universita dell Aquila, L'Aquila, Italy; #corresponding author *Dedicated to the memory of Professor Alexander J. Friedenstein, pioneer of bone marrow stromal cell research, inspiring teacher and friend.ABSTRACT: The bone marrow stroma consists of a heterogeneous population of cells that provide the structural and physiological support for hematopoietic cells. Additionally, the bone marrow stroma contains cells with a stem-cell-like character that allows them to differentiate into bone, cartilage, adipocytes, and hematopoietic supporting tissues. Several experimental approaches have been used to characterize the development and functional nature of these cells in vivo and their differentiating potential In vitro. In vivo, presumptive osteogenic precursors have been identified by morphologic and immunohistochemical methods. In culture, the stromal cells can be separated from hematopoietic cells by their differential adhesion to tissue culture plastic and their prolonged proliferative potential. In cultures generated from single-cell suspensions of marrow, bone marrow stromal cells grow in colonies, each derived from a single precursor cell termed the colony-forming unit-fibroblast. Culture methods have been developed to expand marrow stromal cells derived from human, mouse, and other species. Under appropriate conditions, these cells are capable of forming new bone after in vivo transplantation. Various methods of cultivation and transplantation conditions have been studied and found to have substantial influence on the transplantation outcome. The finding that bone marrow stromal cells can be manipulated in vitro and subsequently form bone in vivo provides a powerful new model system for studying the basic biology of bone and for generating models for therapeutic strategies aimed at regenerating skeletal elements,

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Osteogenic properties of adhesive cells from mouse bone marrow Dexter cultures

Cited by 9 publications

References 4 publications

Expansion of Murine Periosteal Progenitor Cells with Fibroblast Growth Factor 2 Reveals an Intrinsic Endochondral Ossification Program Mediated by Bone Morphogenetic Protein 2

Expansion of Murine Periosteal Progenitor Cells with Fibroblast Growth Factor 2 Reveals an Intrinsic Endochondral Ossification Program Mediated by Bone Morphogenetic Protein 2

In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape

Bone Marrow Stromal Cells: Characterization and Clinical Application

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