In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape

Mankani, Mahesh H.; Kuznetsov, Sergei A.; Fowler, Bruce O.; Kingman, Albert; Robey, Pamela Gehron

doi:10.1002/1097-0290(20010105)72:1<96::aid-bit13>3.0.co;2-a

Cited by 181 publications

(153 citation statements)

References 28 publications

(22 reference statements)

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“…The osteogenic stromal cells in marrow can undergo osteogenesis when exposed to the TCP content of the construct, and build bony replacement while PLGA degrades. [34][35][36] Although many designs of osteochondral constructs have included pre-seeding of cells to build the osseous phase, 16,21,37 this is unnecessary for intraarticular application. 38 We observed bone formation in the osseous phase of all specimens, with evident subchondral remodeling.…”

Section: Discussionmentioning

confidence: 99%

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Jiang

Chiang

Liao³

et al. 2007

Journal Orthopaedic Research

136

105

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Autologous chondrocyte implantation (ACI) has been recently used to treat cartilage defects. Partly because of the success of mosaicplasty, a procedure that involves the implantation of native osteochondral plugs, it is of potential significance to consider the application of ACI in the form of biphasic osteochondral composites. To test the clinical applicability of such composite construct, we repaired osteochondral defect with ACI at low cell-seeding density on a biphasic scaffold, and combined graft harvest and implantation in a single surgery. We fabricated a biphasic cylindrical porous plug of DL-poly-lactide-co-glycolide, with its lower body impregnated with b-tricalcium phosphate as the osseous phase. Osteochondral defects were surgically created at the weight-bearing surface of femoral condyles of Lee-Sung mini-pigs. Autologous chondrocytes isolated from the cartilage were seeded into the upper, chondral phase of the plug, which was inserted by press-fitting to fill the defect. Defects treated with cell-free plugs served as control. Outcome of repair was examined 6 months after surgery. In the osseous phase, the biomaterial retained in the center and cancellous bone formed in the periphery, integrating well with native subchondral bone with extensive remodeling, as depicted on X-ray roentgenography by higher radiolucency. In the chondral phase, collagen type II immunohistochemistry and Safranin O histological staining showed hyaline cartilage regeneration in the experimental group, whereas only fibrous tissue formed in the control group. On the International Cartilage Repair Society Scale, the experimental group had higher mean scores in surface, matrix, cell distribution, and cell viability than control, but was comparable with the control group in subchondral bone and mineralization. Tensile stress-relaxation behavior determined by uni-axial indentation test revealed similar creep property between the surface of the experimental specimen and native cartilage, but not the control specimen. Implanted autologous chondrocytes could survive and could yield hyaline-like cartilage in vivo in the biphasic biomaterial construct. Pre-seeding of osteogenic cells did not appear to be necessary to regenerate subchondral bone. ß

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Section: Discussionmentioning

confidence: 99%

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Jiang

Chiang

Liao³

et al. 2007

Journal Orthopaedic Research

136

105

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“…Gel-like matrices such as fibrin have been used for cell immobilization in combination with other scaffolds [69]. While a variety of highly innovative matrices are under in vitro and in vivo evaluation, clinically established and approved biomaterials are readily available for first applications of bioartificial bone tissues [70][71][72][73][74][75][76][77]. Computer assisted design -computer assisted manufacturing (CAD/CAM) and rapid prototyping techniques allow the generation of custom-made scaffolds for cell delivery that fit into certain bone defects [77,78].…”

Section: Components Of Bioartificial Bone Tissues Scaffoldsmentioning

confidence: 99%

Tissue engineering of bone: the reconstructive surgeon's point of view

Kneser¹,

Schaefer

Polykandriotis³

et al. 2006

J Cellular Molecular Medi

461

350

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Bone defects represent a medical and socioeconomic challenge. Different types of biomaterials are applied for reconstructive indications and receive rising interest. However, autologous bone grafts are still considered as the gold standard for reconstruction of extended bone defects. The generation of bioartificial bone tissues may help to overcome the problems related to donor site morbidity and size limitations. Tissue engineering is, according to its historic definition, an "interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function". It is based on the understanding of tissue formation and regeneration and aims to rather grow new functional tissues than to build new spare parts. While reconstruction of small to moderate sized bone defects using engineered bone tissues is technically feasible, and some of the currently developed concepts may represent alternatives to autologous bone grafts for certain clinical conditions, the reconstruction of largevolume defects remains challenging. Therefore vascularization concepts gain on interest and the combination of tissue engineering approaches with flap prefabrication techniques may eventually allow application of bone-tissue substitutes grown in vivo with the advantage of minimal donor site morbidity as compared to conventional vascularized bone grafts. The scope of this review is the introduction of basic principles and different components of engineered bioartificial bone tissues with a strong focus on clinical applications in reconstructive surgery. Concepts for the induction of axial vascularization in engineered bone tissues as well as potential clinical applications are discussed in detail.

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“…It was found that the ability of human adult MSCs to form bone is dependent upon osteoinductive carriers such as HA/TCP (hydroxyapatite/tricalcium phosphate) that are nonbiodegradable. 38 Consequently, genetic engineering of human adult MSCs may elicit the osteogenic potential of adult MSCs regardless of the carrier type, with the use of biodegradable carriers. 35,39 Therefore, human adult MSCs possess therapeutic potential even without genetic engineering.…”

Section: Bone Marrow Adult Mscs For Bone Gene Therapymentioning

confidence: 99%

Stem cells as vehicles for orthopedic gene therapy

et al. 2004

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Adult stem cells reside in adult tissues and serve as the source for their specialized cells. In response to specific factors and signals, adult stem cells can differentiate and give rise to functional tissue specialized cells. Adult mesenchymal stem cells (MSCs) have the potential to differentiate into various mesenchymal lineages such as muscle, bone, cartilage, fat, tendon and ligaments. Adult MSCs can be relatively easily isolated from different tissues such as bone marrow, fat and muscle. Adult MSCs are also easy to manipulate and expand in vitro. It is these properties of adult MSCs that have made them the focus of cellmediated gene therapy for skeletal tissue regeneration. Adult MSCs engineered to express various factors not only deliver them in vivo, but also respond to these factors and differentiate into skeletal specialized cells. This allows them to actively participate in the tissue regeneration process. In this review, we examine the recent achievements and developments in stem-cell-based gene therapy approaches and their applications to bone, cartilage, tendon and ligament tissues that are the current focus of orthopedic medicine.

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In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape

Cited by 181 publications

References 28 publications

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Repair of porcine articular cartilage defect with a biphasic osteochondral composite

Tissue engineering of bone: the reconstructive surgeon's point of view

Stem cells as vehicles for orthopedic gene therapy

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