Osteoblast response to the elastic strain of metallic support

Lewandowska-Szumieł, Małgorzata; Sikorski, K.; Szummer, A.; Lewandowski, Zbigniew; Marczyński, Wojciech

doi:10.1016/j.jbiomech.2006.02.012

Cited by 15 publications

(12 citation statements)

References 23 publications

(27 reference statements)

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“…Several in vitro studies suggest that cell mechanical stimulation promotes proliferation and differentiation of osteogenic lineage [1, 2], in agreement with the results from several in vivo studies [3–5]. Bone remodeling is influenced by strain magnitude, frequency, and loading duration.…”

Section: Introductionsupporting

confidence: 83%

A New Piezoelectric Actuator Induces Bone FormationIn Vivo: A Preliminary Study

Reis

Frias

Castro

et al. 2012

Journal of Biomedicine and Biotechnology

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This in vivo study presents the preliminary results of the use of a novel piezoelectric actuator for orthopedic application. The innovative use of the converse piezoelectric effect to mechanically stimulate bone was achieved with polyvinylidene fluoride actuators implanted in osteotomy cuts in sheep femur and tibia. The biological response around the osteotomies was assessed through histology and histomorphometry in nondecalcified sections and histochemistry and immunohistochemistry in decalcified sections, namely, through Masson's trichrome, and labeling of osteopontin, proliferating cell nuclear antigen, and tartrate-resistant acid phosphatase. After one-month implantation, total bone area and new bone area were significantly higher around actuators when compared to static controls. Bone deposition rate was also significantly higher in the mechanically stimulated areas. In these areas, osteopontin increased expression was observed. The present in vivo study suggests that piezoelectric materials and the converse piezoelectric effect may be used to effectively stimulate bone growth.

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Section: Introductionsupporting

confidence: 83%

A New Piezoelectric Actuator Induces Bone FormationIn Vivo: A Preliminary Study

Reis

Frias

Castro

et al. 2012

Journal of Biomedicine and Biotechnology

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show abstract

“…Several studies have shown a decreasing trend of osteoblastic differentiation when shear and stretching forces were applied via flexible titanium substrates (Bannister et al, 2002;Walboomers et al, 2004;Lewandowska-Szumiel et al, 2007). We found that a 10-to 20-μm range of vibration did not affect osteoblastic proliferation or differentiation negatively.…”

Section: Discussionmentioning

confidence: 55%

Osteoblast Mechanoresponses on Ti with Different Surface Topographies

et al. 2009

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During implant healing, mechanical force is transmitted to osteogenic cells via implant surfaces with various topographies. This study tested a hypothesis that osteoblasts respond to mechanical stimulation differently on titanium with different surface topographies. Rat bone-marrow-derived osteoblastic cells were cultured on titanium disks with machined or acid-etched surfaces. A loading session consisted of a 3-minute application of a 10- or 20-mum-amplitude vibration. Alkaline phosphatase activity and gene expression increased only when the cells were loaded in 3 sessions/day on machined surfaces, regardless of the vibration amplitude, whereas they were increased with 1 loading session/day on the acid-etched surface. The loading did not affect the osteoblast proliferation on either surface, but selectively enhanced the cell spreading on the machined surface. Analysis of the data suggests that osteoblastic differentiation is promoted by mechanical stimulation on titanium, and that the promotion is disproportionate, depending on the titanium surface topography. The frequency of mechanical stimulation, rather than its amplitude, seemed to have a key role.

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“…Lipowiecki and Brabazon (2010) also agree that geometrical scaffold architecture has important consequences in bone regeneration, and also suggest preferential zones of bone regeneration around scaffold struts, where higher stresses are found. This may also be linked with the experimental observation that mesenchymal stem cells tend to differentiate to bone-like cells (osteoblasts) with increasing stiffness of the substrate, which also favours migration (Pavlin et al, 2001;Di Palma et al, 2005;Ignatius et al, 2005;Engler et al, 2006;Tang et al, 2006;Lewandowska-Szumiel et al, 2007).…”

Section: Discussionmentioning

confidence: 89%