Osteoblast Mechanoresponses on Ti with Different Surface Topographies

Abstract

During implant healing, mechanical force is transmitted to osteogenic cells via implant surfaces with various topographies. This study tested a hypothesis that osteoblasts respond to mechanical stimulation differently on titanium with different surface topographies. Rat bone-marrow-derived osteoblastic cells were cultured on titanium disks with machined or acid-etched surfaces. A loading session consisted of a 3-minute application of a 10- or 20-mum-amplitude vibration. Alkaline phosphatase activity and gene expression increased only when the cells were loaded in 3 sessions/day on machined surfaces, regardless of the vibration amplitude, whereas they were increased with 1 loading session/day on the acid-etched surface. The loading did not affect the osteoblast proliferation on either surface, but selectively enhanced the cell spreading on the machined surface. Analysis of the data suggests that osteoblastic differentiation is promoted by mechanical stimulation on titanium, and that the promotion is disproportionate, depending on the titanium surface topography. The frequency of mechanical stimulation, rather than its amplitude, seemed to have a key role.

Cell Differentiation on Nanoscale Features of a Titanium Surface: Effects of Deposition Time in NaOH Solution

“…Therefore, the relationship between early and late cellular responses is very important for current research. It is believed with certainly that osteogenic differentiation can be shown that ALP has an activating effect on the surface modification of materials [15][16][17][18][19][20][21][22][23][24][25][26] . There is also evidence that surface modification of nanostructures can increase calcium deposition 15,16,18,20,26) .…”

Self-assembled rosette nanotubes encapsulate and slowly release dexamethasone

“…1 A variety of materials have been investigated as bone replacements, such as conventional titanium and its alloys, micron grain sized ceramics (eg, hydroxylapatite or HA) and nanostructured hydrogels (eg, poly-lactic-co-glycolic acid or PLGA). [2][3][4][5] However, current conventional implants suffer from limited lifetimes, often failing before the end of a patient's natural life expectancy. 6,7 For example, today's orthopedic implants usually have insufficient initial bone growth on their surface and/or poor maintenance of healthy juxtaposed bone over long periods of time.…”

Biocompatibilidade e osteointegração de células osteoblásticas em contato com nióbio

“…As reações bioquímicas induzidas pelo estímulo mecânico atuam em nível celular e podem causar inibição da apoptose, aumento da proliferação celular, alteração na migração celular, entre outros efeitos [81].…”

“…Este estímulo é transmitido por toda a célula através de uma complexa rede que conecta a membrana plasmática ao núcleo da célula [81].…”

“…Os aumentos no 3º e 7º dia podem estar relacionado ao aumento na expressão do colágeno tipo I e as proteínas transmembranas integrinas α2, visto que a FAK esta associada à sinalização celular de ambas indiretamente, como evidenciado por Sato et al e Castillo et al [81,82].…”