Osteoblast and Gingival Fibroblast Markers in Dental Implant Studies

Pivodová, Veronika; Franková, Jana; Ulrichová, Jitka

doi:10.5507/bp.2011.021

Cited by 37 publications

(38 citation statements)

References 110 publications

(110 reference statements)

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“…Collagen I is mainly produced in the gingival fibroblasts, osteoblasts and periodontal ligament [29,30]. It is an important factor of gingival connective tissue and contributes to rapid periodontal tissue regeneration and maintenance of tissue architecture [31,32]. In this experiment, the mRNA levels of Type I collagen were significantly high on Zir after 24 and 72 h of cell culture ( p < 0.05).…”

Section: Resultsmentioning

confidence: 78%

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

et al. 2015

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It was indicated that tetragonal zirconia polycrystal (TZP) containing yttria (Y2O3) and niobium oxide (Nb2O5) ((Y,Nb)-TZP) could be an adequate dental material to be used at esthetically important sites. The (Y,Nb)-TZP was also proved to possess its osteogenic potential comparable with those conventional dental implant material, titanium (Ti). The objective of the current study was to characterize cellular response of human gingival fibroblasts (HGFs) to smooth and rough surfaces of the (Y,Nb)-TZP disc, which were obtained by polishing and sandblasting, respectively. Various microscopic, biochemical, and molecular techniques were used to investigate the disc surfaces and cellular responses for the experimental (Y,Nb)-TZP and the comparing Ti groups. Sandblasted rough (Y,Nb)-TZP (Zir-R) discs had the highest surface roughness. HGFs cultured on polished (Y,Nb)-TZP (Zir) showed a rounded cell morphology and light spreading at 6 h after seeding and its proliferation rate significantly increased during seven days of culture compared to other surfaces. The mRNA expressions of type I collagen, integrin α2 and β1 were significantly stimulated for the Zir group at 24 h after seeding. The current findings, combined with the previous results, indicate that (Y,Nb)-TZP provides appropriate surface condition for osseointegration at the fixture level and for peri-implant mucosal sealing at the abutment level producing a suitable candidate for dental implantation with an expected favorable clinical outcome.

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Section: Resultsmentioning

confidence: 78%

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

et al. 2015

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“…33,34 SEM images and LIVE/DEAD staining after 24 h of culture showed that fibroblastic and osteoblastic cells receiving photoexcited Au 25 (Capt) 18 clusters normally spread on the culture dish and fluoresced as live cells. In addition, expression of f-actin and vinculin, …”

Section: Discussionmentioning

confidence: 99%

Antimicrobial photodynamic activity and cytocompatibility of Au<sub>25</sub>(Capt)<sub>18</sub> clusters photoexcited by blue LED light irradiation

Miyata¹,

Miyaji²,

Kawasaki³

et al. 2017

IJN

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Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au 25 (Capt) 18 ) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen ( 1 O 2 ). Accordingly, the antimicrobial and cytotoxic effects of Au 25 (Capt) 18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1 O 2 generation of Au 25 (Capt) 18 clusters under blue LED irradiation (420–460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans , and Porphyromonas gingivalis , were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1 O 2 generation ability of Au 25 (Capt) 18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au 25 (Capt) 18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1 O 2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.

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“…It is essential in maintaining tissue structure as the basis of mineral deposition. 25,26 OCN is a late indicator of bone differentiation, usually in matrix maturation and mineralization stage. 27,28 Runx2 is a key transcription factor in osteoblast differentiation pathways.…”

mentioning

confidence: 99%

Osseointegration of layer-by-layer polyelectrolyte multilayers loaded with IGF1 and coated on titanium implant under osteoporotic condition

Huan¹,

Wang²,

Xiao³

et al. 2017

IJN

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Purpose Titanium implant is a widely used method for dental prosthesis restoration. Nevertheless, in patients with systemic diseases, including osteoporosis, diabetes, and cancer, the success rate of the implant is greatly reduced. This study investigates a new implant material loaded with insulin-like growth factor 1 (IGF1), which could potentially improve the implant success rate, accelerate the occurrence of osseointegration, and provide a new strategy for implant treatment in osteoporotic patients. Materials and methods Biofunctionalized polyelectrolyte multilayers (PEMs) with polyethylenimine as the excitation layer and gelatin/chitosan loaded with IGF1 were prepared on the surface of titanium implant by layer-by-layer self-assembly technique. The physical and chemical properties of the biofunctionalized PEMs, the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), and bone implant contact correlation test indexes were detected and analyzed in vitro and in vivo using osteoporosis rat model. Results PEMs coatings loaded with IGF1 (TNS-PEM-IGF1-100) implant promoted the early stage of BMMSCs adhesion. Under the action of body fluids, the active coating showed sustained release of growth factors, which in turn promoted the proliferation and differentiation of BMMSCs and the extracellular matrix. At 8 weeks from implant surgery, the new bone around the implants was examined using micro-CT and acid fuchsin/methylene blue staining. The new bone formation increased with time in each group, while the TNS-PEM-IGF1-100 group showed the highest thickness and continuity. Conclusion TNS-PEM-IGF1-100 new implants can promote osseointegration in osteoporotic conditions both in vivo and in vitro and provide a new strategy for implant repair in osteoporotic patients.

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Osteoblast and Gingival Fibroblast Markers in Dental Implant Studies

Cited by 37 publications

References 110 publications

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

Antimicrobial photodynamic activity and cytocompatibility of Au<sub>25</sub>(Capt)<sub>18</sub> clusters photoexcited by blue LED light irradiation

Osseointegration of layer-by-layer polyelectrolyte multilayers loaded with IGF1 and coated on titanium implant under osteoporotic condition

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