Oscillations in cell morphology and redox state

Visser, G.; Reinten, Charlotte; Coplan, Paul; Gilbert, D.A.; Hammond, Kate

doi:10.1016/0301-4622(90)88037-s

Cited by 25 publications

(13 citation statements)

References 21 publications

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“…This approach is progressively less useful as the spatial complexity of the rhythmic behaviour increases. An alternative strategy is to analyse the image series in the frequency domain and summarise the oscillatory behaviour by particular key attributes, such as the frequency, phase or magnitude (Visser et al, 1990;Gilbert and Visser, 1993;Tlalka et al, 2002;Tlalka et al, 2003;Roussel and Lloyd, 2007). This approach can be extended to a pixel-by-pixel analysis in which the frequency, phase or magnitude are represented as the hue in pseudo-colour coded images.…”

Section: Introductionmentioning

confidence: 98%

Fourier-based spatial mapping of oscillatory phenomena in fungi

Fricker

Tlalka

Bebber

et al. 2007

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 98%

Fourier-based spatial mapping of oscillatory phenomena in fungi

Fricker

Tlalka

Bebber

et al. 2007

Fungal Genetics and Biology

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“…This diagram provides the successive power spectra each showing oscillations with periods of between 11 and 328 s, as obtained at 8 s intervals over a total period of 6,686 s, and showing the effect of additions of 1 µM ATP on (a) cell surface activity (light scattering due to cell surface and “ruffling” undulations, and fluorescence emission bands of: (b) NADH (450 ± 20 nm), and (c) oxidised FAD 520 ± 20 nm metabolic redox rhythms) (Visser, 1993; Visser et al, 1990).…”

Section: Dynamics Of Cellular Differentiation and Oncogenesismentioning

confidence: 99%

An appreciation of the prescience of Don Gilbert (1930–2011): master of the theory and experimental unravelling of biochemical and cellular oscillatory dynamics

Gilbert

Hammond²,

Brodsky

et al. 2020

Cell Biology International

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We review Don Gilbert's pioneering seminal contributions that both detailed the mathematical principles and the experimental demonstration of several of the key dynamic characteristics of life. Long before it became evident to the wider biochemical community, Gilbert proposed that cellular growth and replication necessitate autodynamic occurrence of cycles of oscillations that initiate, coordinate and terminate the processes of growth, during which all components are duplicated and become spatially re‐organised in the progeny. Initiation and suppression of replication exhibit switch‐like characteristics, that is, bifurcations in the values of parameters that separate static and autodynamic behaviour. His limit cycle solutions present models developed in a series of papers reported between 1974 and 1984, and these showed that most or even all of the major facets of the cell division cycle could be accommodated. That the cell division cycle may be timed by a multiple of shorter period (ultradian) rhythms, gave further credence to the central importance of oscillatory phenomena and homeodynamics as evident on multiple time scales (seconds to hours). Further application of the concepts inherent in limit cycle operation as hypothesised by Gilbert more than 50 years ago are now validated as being applicable to oscillatory transcript, metabolite and enzyme levels, cellular differentiation, senescence, cancerous states and cell death. Now, we reiterate especially for students and young colleagues, that these early achievements were even more exceptional, as his own lifetime's work on modelling was continued with experimental work in parallel with his predictions of the major current enterprises of biological research.

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“…However, even with repeated sampling from different ROIs, it was extremely difficult to build up a picture of how these oscillations were organized or propagated over the whole colony by simple comparison of traces over time. We therefore analyzed the image series in the frequency domain and summarized the oscillatory behaviour by mapping particular key attributes, such as the frequency, phase or magnitude, on a pixel‐by‐pixel basis and representing them as the hue in pseudo‐colour‐coded images (Visser et al , 1990; Gilbert & Visser, 1993; Tlalka et al , 2002, 2003; Fricker et al , 2007b; Roussel & Lloyd, 2007; Tlalka et al , 2007). This maintains the spatial information in the image and greatly facilitates understanding of potentially complex propagating waves or development of oscillatory domains with distinct behaviour (see for example Gray et al , 1998; Mrsic‐Flogel et al , 2003; Sawai et al , 2005; Fricker et al , 2007b).…”

Section: Transport At the Millimetre To Centimetre Scalementioning

confidence: 99%

Imaging complex nutrient dynamics in mycelial networks

Fricker

Lee

Bebber

et al. 2008

Journal of Microscopy

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SummaryTransport networks are vital components of multi-cellular organisms, distributing nutrients and removing waste products. Animal cardiovascular and respiratory systems, and plant vasculature, are branching trees whose architecture is thought to determine universal scaling laws in these organisms. In contrast, the transport systems of many multicellular fungi do not fit into this conceptual framework, as they have evolved to explore a patchy environment in search of new resources, rather than ramify through a three-dimensional organism. These fungi grow as a foraging mycelium, formed by the branching and fusion of threadlike hyphae, that gives rise to a complex network. To function efficiently, the mycelial network must both transport nutrients between spatially separated source and sink regions and also maintain its integrity in the face of continuous attack by mycophagous insects or random damage. Here we review the development of novel imaging approaches and software tools that we have used to characterise nutrient transport and network formation in foraging mycelia over a range of spatial scales. On a millimetre scale, we have used a combination of time-lapse confocal imaging and fluorescence recovery after photobleaching to quantify the rate of diffusive transport through the unique vacuole system in individual hyphae. These data then form the basis of a simulation model to predict the impact of such diffusion-based movement on a scale of several millimetres. On a centimetre scale, we have used novel photon-counting scintillation imaging techniques to visualize radiolabel movement in small microcosms. This approach has revealed novel N-transport phenomena, including rapid, preferential N-resource allocation to C-rich sinks, induction

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Oscillations in cell morphology and redox state

Cited by 25 publications

References 21 publications

Fourier-based spatial mapping of oscillatory phenomena in fungi

Fourier-based spatial mapping of oscillatory phenomena in fungi

An appreciation of the prescience of Don Gilbert (1930–2011): master of the theory and experimental unravelling of biochemical and cellular oscillatory dynamics

Imaging complex nutrient dynamics in mycelial networks

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