Fourier-based spatial mapping of oscillatory phenomena in fungi

Emergence of self-organised oscillatory domains in fungal mycelia

“…To visualise differences in the phase of the oscillatory component across the colony, an image processing suite written in MatLab (The Mathworks, Inc., Natick, MA, USA) was used to apply Fourier analysis (Tlalka et al, 2002(Tlalka et al, , 2003) on a pixel-by-pixel basis according to Fricker et al (2007). In brief, the raw data for each pixel was smoothed, de-trended and filtered with a Hanning window to provide a time-series suitable for Fourier analysis.…”

“…Pseudo-colour coded images showing the relative phase of the oscillatory component of 14 C-AIB transport determined pixel-by-pixel using Fourier analysis (seeFricker et al, 2007). The colour represents the phase difference in hours from the oscillation recorded at the loading site.…”

Fungal network responses to grazing

“…However, all these previous transport and network studies with P. velutina have been conducted in small (12 Â 12 cm or 25 Â 25 cm) microcosms from agar or wood block inocula that have been colonised by mycelia growing on rich media Fricker et al, 2007b;Rotheray et al, 2008;Tlalka et al, 2007Tlalka et al, , 2008a. Under these conditions, the initial dense, radial symmetric growth phase tends to reflect the mycelial response to high nutrients that are sequestered by mycelium during the initial colonisation phase (Tlalka et al, 2008a).…”

Quantifying dynamic resource allocation illuminates foraging strategy in Phanerochaete velutina

“…With the development of photon-counting scintillation imaging (PCSI, Tlalka et al, 2002Tlalka et al, , 2003Tlalka et al, , 2007Fricker et al, 2007), it has been possible to map the distribution of the non-metabolised, amino-acid analogue, a-aminoisobutyrate ( 14 C-AIB) during the development of small mycelial networks of Phanerochaete velutina in spatially heterogenous resource environments. We have previously shown that 14 C-AIB was taken up rapidly and distributed throughout the colonies, with a marked pulsatile component to transport that was organised into well demarcated domains that differed in the phase of the oscillations (Tlalka et al, 2003Fricker et al, 2007).…”