Orthopedic implant of a polyhydroxybutyrate (PHB) and hydroxyapatite composite in cats

Abstract

The purpose of this study was to evaluate the tissue response to a 70% polyhydroxybutyrate and 30% hydroxyapatite composite in the form of a bone implant, placed intracortically in the distal metaphyseal of the right femur, and subcutaneous implants in cats. Samples of the composite were implanted subcutaneously in the dorsolumbar region and the distal metaphyseal region of the right femur of the animals. The study used 12 neutered adult mixed breed cats, weighing an average of 3.5kg. The cats were randomly divided into three groups: GI, GII and GIII, according to the length of the assessment period. The assessments of their subcutaneous and bone tissues were performed at 15, 30 and 45 days and at 30, 60 and 90 days, respectively. The subcutaneous and bone reactions to the composites were characterized by granulomatous inflammation with a predominance of macrophages and giant cells. The results showed that the composites triggered a chronic local inflammatory response, despite their clinical acceptance.

Hydroxyapatite-lignin composite as a metallic implant-bone tissue osseointegration improver: experimental study in dogs

“…There was no formation of fibrous capsule and inflammatory infiltration related to the biomaterial at any moment, suggesting biocompatibility. Although in lesser amounts, the biomaterial was still present at the day 150, but there was no evidence of phagocytosis in histological sections, as previously described by BORGES et al (2000) and CARLO et al (2009). ALVES et al (2011, who applied hydroxyapatite-polyhydroxybutyrate composite in cats, found fibrous capsule formation in response to a chronic inflammatory process, suggesting that this type of reaction could generate mechanical failure of the implant.…”

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

“…However, the authors suggested that the osteoinductive properties of PHB should be further analyzed (58). On the other hand, Alves et al (59) observed a chronic local inflammatory response when PHB/HA composites were implanted in cats. Brigham and Sinskey (16) summarized early in vivo biocompatibility studies of different PHA matrices and showed that post-traumatic inflammation following surgical procedures was common around PHA implants.…”

Biomechanical Comparison of Plate-Nail Vs. Plate-Rod for Experimentally-Induced Gap Fractures in ex vivo Canine Femora

“…Proximal and distal epiphyseal ostectomies were performed to allow placement of the specimens in the testing frame. Failure in compression testing was determined when the specimens fractured (Alves et al, 2010). The bending test ( Fig.…”

“…5) was performed in a universal testing machine EMIC DL 30000 model (EMIC DL 30000, EMIC equipamentos e sistemas de ensaios Ltda., São José dos Pinhais, Paraná, Brazil) , with a load cell of 19600 N and displacement speed of 10 mm secG 1 . Failure in bending was considered complete when the specimens ruptured (Alves et al, 2010). The force was applied to the lateral aspect of the femur bone on the plate.…”