Orosomucoid in urine is a powerful predictor of cardiovascular mortality in normoalbuminuric patients with type 2 diabetes at five years of follow-up

Christiansen, Merete Skovdal; Hommel, E.; Magid, Erik; Feldt‐Rasmussen, Bo

doi:10.1007/s00125-004-1630-1

Cited by 29 publications

(18 citation statements)

References 38 publications

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“…An important reason to use suction blister fluid as body fluid in some of these cases, would be that it is much less invasive than biopsies. In these cases, the blisters probably have to be applied near the localized effect [55][56][57]. From Table 1 it becomes clear that various proteins are used to diagnose different diseases and that some proteins can be used to diagnose or monitor multiple diseases.…”

Section: Protein Markers In Suction Blister Fluid Without Additional mentioning

confidence: 99%

Suction blister fluid as potential body fluid for biomarker proteins

et al. 2007

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Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.

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Section: Protein Markers In Suction Blister Fluid Without Additional mentioning

confidence: 99%

Suction blister fluid as potential body fluid for biomarker proteins

et al. 2007

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“…Previously we showed that u-ORM could be a more sensitive marker of inflammation than serum ORM which rises only 2-fold in contrast to even 200-fold increase of u-ORM in sepsis ( 21 ). Others also showed that the elevation of u-ORM is much more considerable than that of serum ORM in relation to inflammatory diseases ( 10 , 11 , 17 ). These results indicate that u-ORM is a reliable marker of chronic and acute inflammatory disorders and provides valuable supplementary information for daily clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Urinary orosomucoid: validation of an automated immune turbidimetric test and its possible clinical use

et al. 2016

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IntroductionBesides routine serum markers of inflammatory diseases, the diagnostic potential of selected urinary proteins has not been fully exploited yet. Former studies revealed that urinary orosomucoid (u-ORM) might have complementary information in inflammatory disorders. Our aim was to develop and validate a fully automated method for u-ORM measurements and to evaluate its potential clinical impact on systemic inflammatory diseases.Materials and methodsA particle-enhanced immune turbidimetric assay was validated for a Cobas 8000/c502 analyzer to determine u-ORM levels. Spot urine samples from 72 healthy individuals, 28 patients with Crohn’s disease and 30 septic patients were studied.ResultsOur assay time was 10 minutes and the detection limit of u-ORM was 0.02 mg/L. The intra- and inter-assay imprecision expressed as CV was less than 5%, and the recovery ranged between 95–103%. Within 10 to 60 years of age, a preliminary reference range for urinary orosomucoid/creatinine ratio (u-ORM/u-CREAT) was found to be 0.08 (0.01–0.24) mg/mmol [median (2.5–97.5 percentiles)]. Compared to controls, a five-fold increase of u-ORM/u-CREAT values in Crohn’s disease and approximately a 240-fold increase in sepsis were observed.ConclusionsWe set up a fast, sensitive and precise turbidimetric approach for automated u-ORM determination. Our highly sensitive assay is ideal for routine u-ORM measurements and might be a potential novel laboratory test in the management of systemic inflammatory processes.

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“…Our subsequent studies showed that orosomucoid is a prominent component of proteinuria in acute heart disease (16 ). Our interest in studying the excretion of urinary orosomucoid in states of acute inflammation was further strengthened by our recent finding that the urinary orosomucoid excretion rate is a strong risk marker for cardiovascular mortality in patients with type 2 diabetes and appears to be a marker of low-grade inflammation in this chronic condition (17,18 ).…”

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confidence: 98%

Monitoring Urinary Orosomucoid in Acute Inflammation: Observations on Urinary Excretion of Orosomucoid, Albumin, α1-Microglobulin, and IgG

et al. 2005

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Background: Inflammation-associated proteinuria in acute, nonrenal disease is a common but poorly understood phenomenon. We performed an observational study of the urinary excretion of orosomucoid (␣ 1 -acid glycoprotein), albumin, ␣ 1 -microglobulin (protein HC), and IgG to obtain quantitative and temporal data on these 4 proteins. Methods: Urine samples were collected at daily intervals for up to 23 days from 6 patients with surgeryinduced inflammation and at hourly intervals for a 24-h period from 7 sepsis patients. Urinary protein concentrations were assessed by immunoturbidimetry. Results: During surgery-induced inflammation, the increase and decrease in orosomucoid excretion mirrored changes in plasma C-reactive protein. Values for all 4 urinary proteins were increased in sepsis patients. The observed maximum increases in urinary protein excretion relative to the upper reference values were 280-fold for orosomucoid, 98-fold for ␣ 1 -microglobulin, 33-fold for albumin, and 26-fold for IgG. Conclusions: Orosomucoid, usually present in plasma and urine in much lower concentrations than albumin, is increased in urine to concentrations equal to or higher than albumin in proteinuria associated with acute inflammation. The pathophysiologic mechanisms responsible for this markedly increased excretion are unknown. Monitoring of urinary excretion of orosomucoid and other specific proteins, expressed as protein/creatinine ratios, may provide a window for clinically rele-

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Orosomucoid in urine is a powerful predictor of cardiovascular mortality in normoalbuminuric patients with type 2 diabetes at five years of follow-up

Cited by 29 publications

References 38 publications

Suction blister fluid as potential body fluid for biomarker proteins

Suction blister fluid as potential body fluid for biomarker proteins

Urinary orosomucoid: validation of an automated immune turbidimetric test and its possible clinical use

Monitoring Urinary Orosomucoid in Acute Inflammation: Observations on Urinary Excretion of Orosomucoid, Albumin, α1-Microglobulin, and IgG

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