Suction blister fluid as potential body fluid for biomarker proteins

Abstract

Early diagnosis is important for effective disease management. Measurement of biomarkers present at the local level of the skin could be advantageous in facilitating the diagnostic process. The analysis of the proteome of suction blister fluid, representative for the interstitial fluid of the skin, is therefore a desirable first step in the search for potential biomarkers involved in biological pathways of particular diseases. Here, we describe a global analysis of the suction blister fluid proteome as potential body fluid for biomarker proteins. The suction blister fluid proteome was compared with a serum proteome analyzed using identical protocols. By using stringent criteria allowing less than 1% false positive identifications, we were able to detect, using identical experimental conditions and amount of starting material, 401 proteins in suction blister fluid and 240 proteins in serum. As a major result of our analysis we construct a prejudiced list of 34 proteins, relatively highly and uniquely detected in suction blister fluid as compared to serum, with established and putative characteristics as biomarkers. We conclude that suction blister fluid might potentially serve as a good alternative biomarker body fluid for diseases that involve the skin.

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

“…Some of the first studies were those of Celis and co-workers in breast carcinomas (80) and adipose tissue from breast carcinoma patients (81) using the tissue elution method, that has later also been used, e.g., in liver (443). A proteomics approach has also been applied on IF isolated by centrifugation in rat bone marrow (488), pig skin (68) and human ovarian carcinomas (184), by capillary ultrafiltration in fibrosarcomas in mice (204), a human head and neck squamous carcinoma (440) and by the suction blister technique in human skin (248). The available data suggest that this is an emerging area with significant potential.…”

Identification of miR‐126 as a new regulator of skin ageing

“…6 Quantitative PCR-based microRNA profiling was performed, and 175 miRs were detected with a C t -value <40 which was sufficient to be considered in the following analysis ( Figure S1 and Table S1). Setting a threshold of 1.…”

“…6 Several studies demonstrated the role of PAR-2 signalling in the process of pigmentation in various experimental conditions (S11-14). In addition, downregulated PAR-2 expression was reported in vitiligo lesional skin, which is associated with melanin loss in keratinocytes.…”

Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface

“…SmTOR might be essential at that specific time-point. Many complement proteins are produced by epidermal cells (Timar et al 2007) and several authors have shown that complement proteins diffuse from the vascular compartment to the dermis and epidermis (Kool et al 2007 ;Hansell et al 2008). The complete complement cascade is active in the skin as indicated by the role of complement in many human blistering skin diseases (Lessey et al 2008).…”