Ornithinimicrobium flavum sp. nov., isolated from the leaf of Paris polyphylla

Abstract

A Gram-positive bacterium originating from the surface-sterilized leaf of Paris polyphylla var. yunnanensis (Franch.) was characterized by using a polyphasic approach. The isolate formed yellow, smooth, circular colonies on nutrient agar with 0.2 % starch (NSA). Cells were non-motile, non-sporulating, irregular rods or cocci. Strain CPCC 203535 had the highest 16S rRNA gene sequence similarity to the type strain of Ornithinimicrobium kibberense (96.9 %) and formed the deepest branch in the genus Ornithinimicrobium in the neighbour-joining (NJ) phylogenetic tree based on 16S rRNA gene sequences. The major menaquinones of strain CPCC 203535 were MK-8(H4), MK-8(H2) and MK-8. The peptidoglycan contained ornithine as the diagnostic diamino acid. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unknown lipid (UL). The major fatty acids iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0 were consistent with the fatty acid patterns reported for members of the genus Ornithinimicrobium. The DNA G+C composition is 71.4 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain CPCC 203535 from its closest phylogenetic species in the genus Ornithinimicrobium. Strain CPCC 203535 represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium flavum sp. nov. is proposed, with CPCC 203535 (=NBRC 109452 =KCTC 29164) as the type strain.

Ornithinimicrobium cerasi sp. nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium

“…At the time of writing, the genus Ornithinimicrobium currently accommodates seven validly and one not-yet validly named species (http://www.bacterio.net/ and https://www.ncbi.nlm.nih.gov/pubmed). Members of the genus were isolated from soil [1, 7], cold desert [10], activated sludge [5], indoor wall [3], water sample [4], medicinal plant [8] and green alga [6]. In the present study, we report polyphasic characterization of another endophytic strain CPCC 203383 T and propose a novel species.…”

“…The cellular fatty acid profile is distinctly dominated by iso - and anteiso -branched-chain acids [9]. DNA G+C contents of species of the genus Ornithinimicrobium range from 68.3 to 71.4 mol% [1, 4, 5, 7, 8, 10]. At the time of writing, the genus Ornithinimicrobium currently accommodates seven validly and one not-yet validly named species (http://www.bacterio.net/ and https://www.ncbi.nlm.nih.gov/pubmed).…”

“…nov. [2]. Species of genus Ornithinimicrobium are characterized by the presence of MK-8(H 4 ) as the major menaquinone and diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the phospholipids [1,[3][4][5][6][7][8]. The cellular fatty acid profile is distinctly dominated by isoand anteiso-branched-chain acids [9].…”

Ornithinimicrobium pratense sp. nov., isolated from meadow soil

“…As of August 2020, the genus Ornithinimicrobium accommodates ten recognized species (https://lpsn.dsmz.de/genus/ornithinimicrobium) [3]. Species of this genus have been isolated from various sources, such as garden soil [1], cold desert soil sample [4], activated sludge of a wastewater treatment bioreactor [5], indoor wall [6], water sample of a recirculating aquaculture system [7], green alga [8], the leaf of Paris polyphylla [9], cultivated ginseng soil [10], karst cave [11] and the fruit of Cerasus pseudocerasus [12]. Generally, members of the genus Ornithinimicrobium are characterized as Gram-stain-positive, having MK-8 (H 4 ) as the major menaquinone, and containing ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan [1, 4–12].…”

“…Species of this genus have been isolated from various sources, such as garden soil [1], cold desert soil sample [4], activated sludge of a wastewater treatment bioreactor [5], indoor wall [6], water sample of a recirculating aquaculture system [7], green alga [8], the leaf of Paris polyphylla [9], cultivated ginseng soil [10], karst cave [11] and the fruit of Cerasus pseudocerasus [12]. Generally, members of the genus Ornithinimicrobium are character-ized as Gram-stain-positive, having MK-8 (H 4 ) as the major menaquinone, and containing ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan [1,[4][5][6][7][8][9][10][11][12]. Here, we report the polyphasic characterization of a Gram-stainpositive bacterium, strain W204 T , as representing a novel species of the genus Ornithinimicrobium.…”