Ornithinimicrobium cerasi sp. nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium

Abstract

Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.) G. Don, was taxonomically characterized based on a polyphasic investigation. It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species. The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium , but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species. The menaquinone was MK-8(H4). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs). The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp. The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp. nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain. The DNA G+C composition is 72.3 mol%. The availability of new data allows for an emended description of the genus Ornithinimicrobium .

Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica

“… *Data taken from references [7, 12]. †Data taken from references [3, 8, 10]. ‡Data taken from references [1, 6–8].…”

“…S5). DPG, PG and PI were the major and common polar lipids of strain Arc0846-15 T and other members within the genus Ornithinimicrobium [1, 3, 4, 7, 9–12]. In particular, PI was absent in species O.…”

“…The DNA G+C contents of species of the genus Ornithinimicrobium range from 68.3 to 71.4 mol% [1,[3][4][5][6][7][8][9][10][11][12]. Members of the genus were isolated from diverse habitats including soils [1,5,6,12], plants [3,4], indoor wall [7], water sample [8], cold desert [10], activated sludge [9] and green alga [11]. During the course of a study on alginate-degrading micro-organisms, a bacterial strain (designated Arc0846-15 T ) representing a novel member of the genus Ornithinimicrobium was isolated and described.…”

Ornithinimicrobium pratense sp. nov., isolated from meadow soil

“…As of August 2020, the genus Ornithinimicrobium accommodates ten recognized species (https://lpsn.dsmz.de/genus/ornithinimicrobium) [3]. Species of this genus have been isolated from various sources, such as garden soil [1], cold desert soil sample [4], activated sludge of a wastewater treatment bioreactor [5], indoor wall [6], water sample of a recirculating aquaculture system [7], green alga [8], the leaf of Paris polyphylla [9], cultivated ginseng soil [10], karst cave [11] and the fruit of Cerasus pseudocerasus [12]. Generally, members of the genus Ornithinimicrobium are characterized as Gram-stain-positive, having MK-8 (H 4 ) as the major menaquinone, and containing ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan [1, 4–12].…”

“…Species of this genus have been isolated from various sources, such as garden soil [1], cold desert soil sample [4], activated sludge of a wastewater treatment bioreactor [5], indoor wall [6], water sample of a recirculating aquaculture system [7], green alga [8], the leaf of Paris polyphylla [9], cultivated ginseng soil [10], karst cave [11] and the fruit of Cerasus pseudocerasus [12]. Generally, members of the genus Ornithinimicrobium are character-ized as Gram-stain-positive, having MK-8 (H 4 ) as the major menaquinone, and containing ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan [1,[4][5][6][7][8][9][10][11][12]. Here, we report the polyphasic characterization of a Gram-stainpositive bacterium, strain W204 T , as representing a novel species of the genus Ornithinimicrobium.…”