Original paper Evaluation of the role of Epstein-Barr virus in cases of nodal or extranodal T- and NK-cell lymphoma using eber in situ hybridization

Karaarslan, Serap; Hekımgıl, Mıne; Soydan, Saliha; Ertan, Yeşim; Doğanavşargıl, Başak

doi:10.5114/pjp.2015.53013

Cited by 14 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 With a cut-off value of > 25% EBER-positive neoplastic cells, EBV positivity of PTCL-NOS was reported to be 5-31%. 21,22 On the other hand, EBV was detected in 6% of PTCL-NOS with a cut-off value of > 50% EBER-positive neoplastic cells. 21 The clinicopathological features of nodal EBV + CTL in previous studies (more than 10 cases) are summarized in Table 2.…”

Section: Epidemiologymentioning

confidence: 99%

Nodal EBV+ cytotoxic T-cell lymphoma: A literature review based on the 2017 WHO classification

Kato

Yamashita

Nakamura

2020

JCEH

View full text Add to dashboard Cite

Peripheral T-cell lymphoma not otherwise specified (PTCL-NOS) accounts for the largest proportion of mature T-cell and natural killer (NK)-cell neoplasms in the 2017 World Health Organization (WHO) classification. 1 We have found evidence that nodal cytotoxic molecule (CM)-positive PTCL-NOS [(also called cytotoxic T-cell lymphoma (CTL)] constitutes a unique category. This group encompasses a wide spectrum of presentations, ranging from indolent disease in a minority to an aggressive neoplasm in the majority; in 45-51% of CTL cases, the pathogenesis is closely associated with Epstein-Barr virus (EBV). 2,3 These biological properties are in clear contrast to those of nodal CM-negative T-cell neoplasms, which predominantly comprise PTCL-NOS, angioimmunoblastic T-cell lymphoma, and adult T-cell leukemia (ATLL, positive for human T-cell leukemia virus type 1)-although a small subset of patients (2%) may exhibit EBV-positive bystander lymphocytes. 2 This suggests that EBV is detected as the result of immunological deterioration in the tumor microenvironment, but that it plays little role in the pathogenesis of the latter neoplasm.Among mature T/NK-cell tumors, the detection of EBV on tumor cells is essential for the diagnosis of nasal type NK/ T-cell lymphoma (NKTL), aggressive NK-cell leukemia, or EBV + T/NK-cell lymphoproliferative diseases of childhood-all of which exhibit CM expression and frequent extranodal involvement. 4 In most NKTL cases, the prototypic disease initially presents in the upper aerodigestive tract. This disease can also involve a wide variety of extranodal sites, including the skin and gastrointestinal tract, and may exhibit secondary lymph node lesions. Indeed, NKTL affecting middle-aged or elderly patients may feature de novo symptoms with clonal and rapid expansion of neoplastic EBV + T/NK cells. 5 Patients with nodal EBV + CTL present with lymphadenopathy without nasopharyngeal involvement. However, this nodal lymphoma and NKTL share some biological properties, including CM expression and EBV positivity. We first elucidated the clinicopathological characteristics of nodal EBV + CTL, which is characterized by diffuse monomorphic infiltration of large cells that often have a centroblastoid

show abstract

Section: Epidemiologymentioning

confidence: 99%

Nodal EBV+ cytotoxic T-cell lymphoma: A literature review based on the 2017 WHO classification

Kato

Yamashita

Nakamura

2020

JCEH

View full text Add to dashboard Cite

show abstract

“…ere were 17 cases graded as 4+, representing >75% EBER-positive neoplastic cells. Other ISH studies have reported EBER reactivity in 22.6% (14/62) of nodal or extranodal T-and NK-cell lymphomas cases [13], with the highest reactivity (30.1%) being in breast tumors [27] and the lowest reactivity (10%) being in gastric tumors (10%) [28]. Considering the strong Table 4: e relationship between the grade and tumor basal dimension in EBER-positive SGC of the eyelid.…”

Section: Discussionmentioning

confidence: 93%

“…e images were photographed using a digital camera attached to an Olympus BX1 light microscope (Tokyo, Japan). Referring to the previous article [13], the criteria used in scoring and grading the intensity of the EBER staining is as follows. (Figure 1 Grades 2+, 3+, and 4+, with a cutoff value of >5% EBERpositive neoplastic cells, were considered EBV positive, and grade 1+, with inadequate or equivocal positive neoplastic cells (a cut-off value <5%), were excluded from this study.…”

Section: Detection Of Ebermentioning

confidence: 99%

Detection of Epstein-Barr Virus in 130 Cases of Eyelid Sebaceous Gland Carcinoma Using In Situ Hybridization

Gao

Tang

Lin

et al. 2020

Journal of Ophthalmology

View full text Add to dashboard Cite

Purpose. In this study, we aimed to investigate the presence of Epstein–Barr virus (EBV) in the eyelid sebaceous gland carcinoma (SGC) and its association with the clinicopathologic features. Methods. One hundred and thirty paraffin-embedded SGC specimens were retrieved from the Clinical Pathology Department of Zhongshan Ophthalmic Center. Epstein–Barr virus-encoded RNA (EBER) was detected with in situ hybridization (ISH) using the Leica BOND system autostainer. The age and gender distributions of all patients were analyzed and compared with earlier reports. Pearson’s χ2 and Fisher’s exact tests were used to determine the association between clinicopathological features such as age, gender, laterality eye, tumor basal dimension, degree of tumor differentiation, and EBER positivity. Likewise, the relationship between the grade and tumor basal dimension in EBER-positive SGC of the eyelid was analyzed. Results. Thirty-four out of one hundred and thirty (26.2%) eyelid SGC specimens were positively stained for EBER. The age range of highest incidence was 46–75 years, and the female to male ratio was 1 : 0.9. No significant correlation was found between EBER-positivity and age (p=0.5370), gender (p=0.4758), and degree of tumor differentiation (p=0.7787). However, EBV positivity was strongly correlated with the right eye (p=0.0287), the tumor basal dimension (p=0.0001). EBV positivity grade presented statistically associated with tumor size (p=0.0329). Conclusion. We conclude that ISH is a sensitive method to identify EBV in SGC of the eyelid. A possible causal association of EBV in SGC patients is suggested by high frequency of EBER-ISH positivity and its association with the clinicopathologic features.

show abstract

“…Compared to conventional plain radiography, CT scanning has become a valuable tool for diagnosing, comparing, and staging of ENKTL [ 14 ]. Many studies have pointed out that LMP1 may serve as a potential indicator for ENKTL [ 27 – 29 ]. Therefore, we aimed to investigate whether ENKTL can differentiate between nasal polyps and inverted papilloma arising in the nasal cavity by comparison of the CT imaging and the expression of LMP1.…”

Section: Discussionmentioning

confidence: 99%

Specific Soft-Tissue Invasion and LMP1 Expression Are Potential Indicators of Extranodal NK/T Cell Lymphoma, Nasal Type

Jiang

Lü

et al. 2018

Med Sci Monit

View full text Add to dashboard Cite

BackgroundExtranodal NK/T cell lymphoma, nasal type (ENKTL-NT) is difficult to distinguish from nasal polyps and inverted papilloma, leading to its high misdiagnosis ratio. The aim of this study was to investigate its potential prognostic indicators.Material/MethodsKaplan-Meier method was used to calculate overall survival (OS) rate. Cox proportional hazards regression was used to analyze risk ratios (ORs) with 95% confidence intervals (CIs).ResultsNasal ala infiltration and nasal floor thickness >2.0 mm or nasal septum thickness >2.5 mm were potential prognostic factors for OS (p=0.0323 and 0.0072, respectively). Cox proportional-hazards regression indicated that high LMP1 expression and the nasal floor thickness >2.0 mm or nasal septum thickness >2.5 mm were the independent risk factors for poor OS of ENKTL-NT (HR=3.0655, p=0.028; HR=2.3650, p=0.0452, respectively). In the subgroup analysis, the OS rate was lower when the nasal floor thickness >2.0 mm or nasal septum thickness >2.5 mm in the patients who had high expression of LMP1 (p=0.0651), whereas high LMP1 expression increased the risk of worse prognostic outcome in patients with deep infiltration thickness. Thus, high LMP1 expression may contribute to the tissue invasion of ENKTL-NT.ConclusionsAny patient with nasal ala soft-tissue invasion, nasal floor thickness >2.0 mm/nasal septum thickness >2.5 mm on CT imaging or high LMP1 expression should prompt immediate histopathologic diagnosis to rule out ENKTL-NT in clinical practice.

show abstract

Original paper Evaluation of the role of Epstein-Barr virus in cases of nodal or extranodal T- and NK-cell lymphoma using eber in situ hybridization

Cited by 14 publications

References 31 publications

Nodal EBV+ cytotoxic T-cell lymphoma: A literature review based on the 2017 WHO classification

Nodal EBV+ cytotoxic T-cell lymphoma: A literature review based on the 2017 WHO classification

Detection of Epstein-Barr Virus in 130 Cases of Eyelid Sebaceous Gland Carcinoma Using In Situ Hybridization

Specific Soft-Tissue Invasion and LMP1 Expression Are Potential Indicators of Extranodal NK/T Cell Lymphoma, Nasal Type

Contact Info

Product

Resources

About