Oriented Nanofibrous Polymer Scaffolds Containing Protein‐Loaded Porous Silicon Generated by Spray Nebulization

Abstract

Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 3.6%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.

Enhance the biocompatibility and osseointegration of polyethylene terephthalate ligament by plasma spraying with hydroxyapatite in vitro and in vivo

“…The average number of nanoparticles and the distribution of diameter were evaluated. 23 Chemical elemental analysis of HA coated on the surface of PET sheet was detected by…”

Oriented Neural Spheroid Formation and Differentiation of Neural Stem Cells Guided by Anisotropic Inverse Opals

“…The topography of these materials influences the mechanosensory apparatus and the spatiotemporal dynamics of the cells ( Chen et al, 2014 ), and these cell-material interactions play a key factor in cell behavior regulation ( Guilak et al, 2009 ; Zangi et al, 2016 ). Many kinds of biomaterials have been investigated for guiding cell growth through topography, including nanofibers ( Liu et al, 2010 ; Xie et al, 2014 ; Omidinia-Anarkoli et al, 2017 ; Zuidema et al, 2018 ; Li et al, 2019 ), colloidal nanoparticles ( Antman-Passig et al, 2017 ; Musoke-Zawedde and Shoichet, 2006 ), and inverse opal materials ( Lu et al, 2014 ; Shang et al, 2019 ; Li et al, 2020 ). Among the applied biomaterials, inverse opal materials represent a class of porous structures with an ordered array of uniform nanoscale or microscale pores, which possessed well-controlled pore size, long-range ordered structure, and homogeneous interconnectivity.…”

Vastly extended drug release from poly(pro-17β-estradiol) materials facilitates in vitro neurotrophism and neuroprotection

“…Electrospun fibers can also be designed to deliver drugs, typically by non-covalently blending the native drug into the electrospinning solution, which allows for passive release profiles via diffusion. In instances where prolonged drug release is required to target persistent injury or degenerative disease, release kinetics are commonly modified by (1) polymer materials selection from known/available supplies 34 , (2) creating dual polymer systems via coaxial electrospinning or incorporating particles into fibers 35–37 , and (3) immobilizing drug to the fiber surface 38 . However, the timescale of drug release still remains quite restricted.…”