Oriented Neural Spheroid Formation and Differentiation of Neural Stem Cells Guided by Anisotropic Inverse Opals

Xia, Lin; Shang, Yixuan; Chen, Xiangbo; He, Li; Xu, Xiaochen; Liu, Wei; Yang, Guang; Gao, Xia; Chai, Renjie

doi:10.3389/fbioe.2020.00848

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…Previously it has been shown that PVDF can be used for attachment and differentiation of cells from different tissues: cardiovascular [ 26 ], osteogenic [ 25 ], muscle [ 62 ], and neuronal cells [ 63 ]. The neural crest stem cells (bNCSC) adhesion, survival, and differentiation were compatible with the PVDF material in line with previous findings on hippocampal neurospheres [ 27 ]. However, the effect of electromagnetic activities on neurogenesis remains controversial.…”

Section: Resultssupporting

confidence: 88%

“…The physical stimulation of stem cell differentiation can replace the biochemical methods that are being used at the current time in stem cell-based therapy of neurodegenerative disorders [ 24 ]. Differentiation of stem cells into osteocytes [ 25 ], cardiomyocytes [ 26 ], and neural cells [ 27 ], initiated by electrical and mechanical stimulus has been studied. Neural cells are more sensitive to electrical stimulation because of their electric activities.…”

Section: Introductionmentioning

confidence: 99%

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Boosting Magnetoelectric Effect in Polymer-Based Nanocomposites

et al. 2021

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Polymer-based magnetoelectric composite materials have attracted a lot of attention due to their high potential in various types of applications as magnetic field sensors, energy harvesting, and biomedical devices. Current researches are focused on the increase in the efficiency of magnetoelectric transformation. In this work, a new strategy of arrangement of clusters of magnetic nanoparticles by an external magnetic field in PVDF and PFVD-TrFE matrixes is proposed to increase the voltage coefficient (αME) of the magnetoelectric effect. Another strategy is the use of 3-component composites through the inclusion of piezoelectric BaTiO3 particles. Developed strategies allow us to increase the αME value from ~5 mV/cm·Oe for the composite of randomly distributed CoFe2O4 nanoparticles in PVDF matrix to ~18.5 mV/cm·Oe for a composite of magnetic particles in PVDF-TrFE matrix with 5%wt of piezoelectric particles. The applicability of such materials as bioactive surface is demonstrated on neural crest stem cell cultures.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Boosting Magnetoelectric Effect in Polymer-Based Nanocomposites

et al. 2021

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“…Owing to the unique advantages of inverse opal scaffolds with regard to pore uniformity, interconnectivity between the cavities, and long-range ordered structure, a number of biomedical applications have been explored, including cell (co-)culture, [69,77] production of cell spheroids, [70,78] cell migration, [79] neovascularization, [71] cardiac tissue engineering, [80] bone/ cartilage/osteochondral tissue engineering, [81][82][83] neural tissue engineering, [84] and wound healing. [85] Among these applications, inverse opal scaffolds are particularly well-suited for bone tissue engineering because of their structural similarity to natural trabecular bone.…”

Section: Functionally Graded Inverse Opal Scaffoldsmentioning

confidence: 99%

Augmenting Tendon‐to‐Bone Repair with Functionally Graded Scaffolds

Zhu

Qiu

Thomopoulos

et al. 2021

Adv Healthcare Materials

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Tendon‐to‐bone repair often fails because the functionally graded attachment is not regenerated during the healing process. Biomimetic scaffolds that recapitulate the unique features of the native tendon‐to‐bone attachment hold great promise for enhancing the healing process. Among various types of scaffolds that are developed and evaluated for tendon‐to‐bone repair, those with gradations (in either a stratified or a continuous fashion) in composition, structure, mechanical properties, and cell phenotype have gained the most attention. In this progress report, the recent efforts in the rational design and fabrication of functionally graded scaffolds based upon electrospun nanofiber mats and inverse opal structures, as well as the evaluation of their applications in augmenting tendon‐to‐bone repair, are reviewed. This report concludes with perspectives on the necessary future steps for clinical translation of the scaffolds.

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“…Hearing loss could be caused by genetic factors, aging, infectious diseases, ototoxic drugs, and noise exposure [1][2][3][4][5][6]. The reported mechanisms of noise-induced hair cells (HCs) and spiral ganglion neuron damage mainly include mechanical shearing forces and oxidative damage to HCs [7] and glutamate excitotoxicity to neurons [8][9][10][11]. In the past, noise exposure was considered harmful only when it causes a per-manent threshold shift (PTS) [3,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Pattern of Cochlear Synaptic Degeneration in C57BL/6J Mice Induced by Repeated Noise Exposure

Qian

Wang

et al. 2021

Neural Plasticity

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It is widely accepted that even a single acute noise exposure at moderate intensity that induces temporary threshold shift (TTS) can result in permanent loss of ribbon synapses between inner hair cells and afferents. However, effects of repeated or chronic noise exposures on the cochlear synapses especially medial olivocochlear (MOC) efferent synapses remain elusive. Based on a weeklong repeated exposure model of bandwidth noise over 2-20 kHz for 2 hours at seven intensities (88 to 106 dB SPL with 3 dB increment per gradient) on C57BL/6J mice, we attempted to explore the dose-response mechanism of prolonged noise-induced audiological dysfunction and cochlear synaptic degeneration. In our results, mice repeatedly exposed to relatively low-intensity noise (88, 91, and 94 dB SPL) showed few changes on auditory brainstem response (ABR), ribbon synapses, or MOC efferent synapses. Notably, repeated moderate-intensity noise exposures (97 and 100 dB SPL) not only caused hearing threshold shifts and the inner hair cell ribbon synaptopathy but also impaired MOC efferent synapses, which might contribute to complex patterns of damages on cochlear function and morphology. However, repeated high-intensity (103 and 106 dB SPL) noise exposures induced PTSs mainly accompanied by damages on cochlear amplifier function of outer hair cells and the inner hair cell ribbon synaptopathy, rather than the MOC efferent synaptic degeneration. Moreover, we observed a frequency-dependent vulnerability of the repeated acoustic trauma-induced cochlear synaptic degeneration. This study provides a sight into the hypothesis that noise-induced cochlear synaptic degeneration involves both afferent (ribbon synapses) and efferent (MOC terminals) pathology. The pattern of dose-dependent pathological changes induced by repeated noise exposure at various intensities provides a possible explanation for the complicated cochlear synaptic degeneration in humans. The underlying mechanisms remain to be studied in the future.

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Oriented Neural Spheroid Formation and Differentiation of Neural Stem Cells Guided by Anisotropic Inverse Opals

Cited by 19 publications

References 39 publications

Boosting Magnetoelectric Effect in Polymer-Based Nanocomposites

Boosting Magnetoelectric Effect in Polymer-Based Nanocomposites

Augmenting Tendon‐to‐Bone Repair with Functionally Graded Scaffolds

Dose-Dependent Pattern of Cochlear Synaptic Degeneration in C57BL/6J Mice Induced by Repeated Noise Exposure

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