Organometric changes of rats thymus after xenobiotics exposure

Shyian, D.; Avilova, Olga; LADNAYA, Irina; Titkova, Anna; Kryzhna, S. I.

doi:10.31688/abmu.2019.54.3.04

Cited by 4 publications

(1 citation statement)

References 5 publications

(5 reference statements)

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“…На будову загруднинної залози різні фактори впливають по-різному, але основний напрямок дії спрямований або на пригнічення її функції, або на стимулювання. Проте все залежить від дози та тривалості впливу [12,13]. Деякі чинники при короткотривалому впливі діють як імуностимулятори, а за довготривалого впливу призводять до виснаження та/або атрофії паре-нхіми тимуса [14,15].…”

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Submicroscopic Changes of Thymus Under Cellular Dehydration

Prykhodko,

Ponyrko

2023

Act. Probl. of the Modern Med.

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Lack of water is one of the most stressful conditions for the body that can lead to changes in all the organs. The importance of the functional state of lymphoid organs for the homeostasis of the whole organism determines the relevance of their thorough investigation under their exposure to various factors, including dehydration. The purpose of the study is to investigate the patterns of submicroscopic changes in the thymus parenchyma of rats under cellular dehydration. Materials and methods. The study was conducted on 45 white mature male rats. The method of electron was applied. Results: After 10 days of the experiment, a mild level of cellular dehydration was observed, during which some cells exhibited signs of apoptosis at various stages. There were detected numerous cells in mitosis, particularly in the cortical substance of thymus lobules and the subcapsular zone. Upon reaching the 20-day mark in the experiment, an intermediate level of cellular dehydration was identified. This stage was characterized by deformations in the nuclei of both small and medium-sized thymocytes, leading to irregularities in their nuclear membrane outlines. After 30 days, under conditions of severe cellular dehydration, a reduction in the number of poorly differentiated lymphoid cells, which serve as precursors to T-lymphocytes, was observed. Thymocytes displayed unclearly defined nuclear contours and increased osmiophilicity, all vessels within the parenchyma of the gland were dilated and filled with blood, some exhibiting damage to the vessel walls, resulting in microhemorrhages, signs of stasis, and sludge syndrome within the lumen. Conclusion: The experiment has demonstrated that after 10-30 days of cellular dehydration, pronounced accidental transformation of thymus occurs, with changes intensifying over time. These findings hold relevance for morphologists and immunologists, as they may be extrapolated to other lymphoid organs under dehydration conditions. Moreover, these results lay the foundation for developing methods to prevent and treat dehydration-related conditions.

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Section: вступunclassified

Submicroscopic Changes of Thymus Under Cellular Dehydration

Prykhodko,

Ponyrko

2023

Act. Probl. of the Modern Med.

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Sıçanların Timusunun Histoultramikroskopik İncelenmesi (Deneysel Veri)

Prykhodko¹,

Dmytruk²,

Avilova

et al. 2021

Düzce Tıp Fakültesi Dergisi

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Aim: The research paper presents the characteristic of cytoarchitectonics of the thymus of intact white mature male laboratory rats. Topicality of the study is due to the need to clarify the data on the contribution of each type of thymus cells in the formation of its structure. The aim of the research was to determine the specifics of localization and ultramicroscopic structure of thymus cells in male mature Wistar laboratory rats. Material and Methods: The study was conducted using histological and ultramicroscopic methods on 10 mature male laboratory rats, weighing 130-150 g. Semi-thin (0.5-1 μm) and ultrathin (0.05-0.2 μm) sections were made on a microtome UMTP-4 (Ukraine), which were stained with 1% methylene blue solution with the addition of 1% sodium tetraborate solution. Histological analysis and photographic recording were performed using Olympus light microscope (Japan) and DSM 510 camcorder with magnification in 1000 times. Results: With a detailed study of the semi-thin and ultrathin sections in the thymus lobules the specifics of localization and ultramicroscopic structure of thymus cells were clearly identified. The features of localization and ultramicroscopic structure of epithelial, mesenchymal, vascular and hematopoietic thymus cells were determined from the point of view of their functional loads and interactions. Conclusion: The described structural peculiarities of the components of the thymus and their relative location in different zones reflect significant organ polymorphism, which must be taken into account in order to achieve the required level of objectivity in the result evaluation of simulated biomedical experiments.

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Structural Changes in the Thymus Under the Pathogenic Factors Action

Prykhodko

2023

VPBM

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Organometric changes of rats thymus after xenobiotics exposure

Cited by 4 publications

References 5 publications

Submicroscopic Changes of Thymus Under Cellular Dehydration

Submicroscopic Changes of Thymus Under Cellular Dehydration

Sıçanların Timusunun Histoultramikroskopik İncelenmesi (Deneysel Veri)

Structural Changes in the Thymus Under the Pathogenic Factors Action

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