Oral treatment with a potential probiotic (<i>Enterococcus faecalis</i>CECT 7121) appears to reduce the parasite burden of mice infected with<i>Toxocara canis</i>

Basualdo, Juan Ángel; Sparo, Mónica; Chiodo, P.; Ciarmela, María Laura; Minvielle, Marta Cecilia

doi:10.1179/136485907x193824

Cited by 36 publications

(29 citation statements)

References 9 publications

(10 reference statements)

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“…In previous research, we indicated that the Ef7121 probiotic reduced the parasite burden in mice infected with T. canis (Basualdo et al 2007). In the present paper, we studied the effect of this strain on the in vitro culture of T. canis larvae and the in vivo persistence of T. canis in the murine intestine.…”

Section: Discussionmentioning

confidence: 91%

“…Ef7121 colonies were identified based on their inhibitory activity against Listeria monocytogenes CEB101 ( Sparo et al 2008). The mesenteric lymph nodes and spleens of inoculated mice were dissected and cultured to evaluate any possible bacterial translocation (Basualdo et al 2007).…”

Section: In Vivo Assaysmentioning

confidence: 99%

“…Our research group has demonstrated that oral treatment with E. faecalis CECT7121 significantly reduces the quantity of larvae recovered from the liver and lungs of mice infected by T. canis (Basualdo et al 2007). To expand this research, the aim of the present study was to evaluate the effect of E. faecalis CECT7121 on the biological cycle of T. canis both in vitro and in vivo.…”

mentioning

confidence: 95%

“…In contrast, Dea-Ayuela et al (2008) (Sparo et al 2006). This strain can adhere and persist in the murine intestinal epithelium (Basualdo et al 2007).…”

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confidence: 99%

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In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Chiodo

Sparo

Pezzani

et al. 2010

Mem. Inst. Oswaldo Cruz

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The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-culture of Ef7121 (EII) and in a fresh culture with Ef7121 (EIII) and the Ef7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of T. canis larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of Ef7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 x 10(4) for Ef7121 (colony forming units/g). At the end of the assay the levels were 1.46 x 10(4). No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. Ef7121 interference with the biological cycle was evaluated in mice challenged with T. canis. The interference was significant when the mice were challenged with probiotic and T. canis simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, Ef7121 possessed in vitro and in vivo larvicidal activity

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Section: Discussionmentioning

confidence: 91%

Section: In Vivo Assaysmentioning

confidence: 99%

mentioning

confidence: 95%

“…In contrast, Dea-Ayuela et al (2008) (Sparo et al 2006). This strain can adhere and persist in the murine intestinal epithelium (Basualdo et al 2007).…”

mentioning

confidence: 99%

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In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Chiodo

Sparo

Pezzani

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…[81,82]. It has also been reported in vivo antiparasitic activity [83], immunomodulation properties [84,85], antitumor action [86] and food biopreserver [80]. AP-CECT7121 is stable at a pH range of 4.0-8.0.…”

Section: Amps Obtained From Gram-positive Bacteriamentioning

confidence: 99%

Bacterial Antagonist Mediated Protein Molecules

Urbizu¹,

Sparo

Bruni³

2015

Clin Exp Pharmacol

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Bacterial antagonism mediated by ribosomally synthesised peptides has gained considerable attention in recent years because of its potential applications in the control of undesirable microbiota. These peptides, generally referred to as bacteriocins, are defined as a heterogeneous group of ribosomally synthesised, proteinaceous substances (with or without further modifications) extracellularly secreted by many Gram-positive and some Gram-negative bacteria. Their mode of activity is primarily bactericidal and directed against closely related strains and species. These peptides are nearly all cationic and very often amphyphilic, which is reflected in the fact that many of these peptides kill their target cells by accumulation in the membrane causing increasing permeability and loss of barrier functions . Bacteriocins have been explored primarily as natural food preservatives, but there is much interest in exploring the application of these therapeutic peptides as antimicrobial agents since many of them exhibit antimicrobial activity against various important human pathogens.The suitability of bacteriocins such as pharmaceuticals is explored through measures of cytotoxicity, effects on the natural microbiota, and in vivo efficacy in mouse models. Bacteriocins are promising therapeutic agents.

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Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice

et al. 2016

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Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes diarrheal disease that is typically self-limiting in immunocompetent hosts but often life threatening to immunocompromised individuals. However, there is a lack of completely efficient therapy available. Probiotics have attracted the attention as potential antiparasite compounds against protozoa involved in intestinal infections. This study investigated the effects of administration of probiotic Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in immunosuppressed mice. Effects on C. parvum infection at the intestinal mucosa were studied and scored at each portion of the gut. It was demonstrated that Ef CECT 7121 interfered with C. parvum infection when both probiotic and parasite were present in the same intestinal location suggesting that Ef CECT 7121 supplementation can alleviate the negative effects of C. parvum infection.

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Oral treatment with a potential probiotic (Enterococcus faecalisCECT 7121) appears to reduce the parasite burden of mice infected withToxocara canis

Abstract: Oral treatment with a potential probiotic (Enterococcus faecalis CECT 7121) appears to reduce the parasite burden of mice infected with Toxocara canis

Cited by 36 publications

References 9 publications

In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Bacterial Antagonist Mediated Protein Molecules

Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice

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