In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Chiodo, P.; Sparo, Mónica; Pezzani, Betina Cecilia; Minvielle, Marta Cecilia; Basualdo, Juan Ángel

doi:10.1590/s0074-02762010000500003

Cited by 29 publications

(21 citation statements)

References 34 publications

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“…Reduction of worm burden was reported in mice infected with Trichinella spiralis 3,4,25 and Toxocara canis 2,7 . The failure of probiotics in affecting parasite load was reported in rats 12 and mice 33 infected with T. spiralis , and finally, an enhancement of mice susceptibility to Trichuris muris was attributed to Lactobacillus casei administration 11 .…”

Section: Discussionmentioning

confidence: 96%

“…Studies on probiotics have revealed that these microorganisms contribute to host defense by reinforcing non-immunological responses and stimulating both specific and non-specific host immunity 14 . Probiotic bacteria have successfully been tested in intestinal protozoan and helmintic infections such as cryptosporidiosis 29 , giardiasis 6 , coccidiosis 9 , trichinellosis 3 and toxocariasis 3,7 .…”

Section: Introductionmentioning

confidence: 99%

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EFFECT OF Bifidobacterium animalis ON MICE INFECTED WITH Strongyloides venezuelensis

Oliveira-Sequeira

David

Ribeiro

et al. 2014

Rev. Inst. Med. trop. S. Paulo

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The administration of viable Bifidobacterium animalis was tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. The oral administration of B. animalis, strain 04450B, starting 14 days before the inoculation of nematode larvae significantly decreased the worm burden and egg output. In probiotic treated animals, the percent reduction of adult worms in the intestine was of 33% and the reduction of egg production was of 21%, compared with those of the control group. The duodenum villous height and villous/crypt ratio were significantly higher in probiotic-treated mice, indicating that this group could be experiencing less intestinal damage. The present findings revealed that the administration of B. animalis for the amelioration of host response to nematode infections is biologically plausible and could have some potential for impacting public health. Meanwhile, further study is needed to delineate the nature and identity of the factor(s) involved in these beneficial effects.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

EFFECT OF Bifidobacterium animalis ON MICE INFECTED WITH Strongyloides venezuelensis

Oliveira-Sequeira

David

Ribeiro

et al. 2014

Rev. Inst. Med. trop. S. Paulo

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“…Also Enterococcus faecalis CECT7121, a probiotic with inhibitory activity against Gram-positive and Gram-negative bacteria, possesses in vitro and in vivo larvicidal activity determining up to a 90% reduction of the number of Toxocara canis larvae in liver and lungs of laboratory mice (Basualdo et al, 2007; Chiodo et al, 2010). Zymomonas mobilis , a bacterium producer of bioethanol, was reported to provide over 60% protection from the infection of S. mansoni in mice (Santos Jde et al, 2004).…”

Section: Probiotics Against Parasitesmentioning

confidence: 99%

Interactions between parasites and microbial communities in the human gut

Berrilli¹,

Cave²,

Cavallero³

et al. 2012

Front. Cell. Inf. Microbio.

117

102

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The interactions between intestinal microbiota, immune system, and pathogens describe the human gut as a complex ecosystem, where all components play a relevant role in modulating each other and in the maintenance of homeostasis. The balance among the gut microbiota and the human body appear to be crucial for health maintenance. Intestinal parasites, both protozoans and helminths, interact with the microbial community modifying the balance between host and commensal microbiota. On the other hand, gut microbiota represents a relevant factor that may strongly interfere with the pathophysiology of the infections. In addition to the function that gut commensal microbiota may have in the processes that determine the survival and the outcome of many parasitic infections, including the production of nutritive macromolecules, also probiotics can play an important role in reducing the pathogenicity of many parasites. On these bases, there is a growing interest in explaining the rationale on the possible interactions between the microbiota, immune response, inflammatory processes, and intestinal parasites.

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“…There was no significant difference between the groups ( p > 0.05)Group104 105 106 107 Overall percentage S. boulardii 97.497.198.997.097.6 B. cereus var toyoi96.896.194.995.195.7 …”

mentioning

confidence: 94%

“…S. boulardii or B. cereus var. toyoi probiotics were then added in triplicate, at concentrations of 1.104, 1.105, 1.106 and 1.107 CFU/hole. The RPMI 1640 has been used and works with these probiotics6 19 and this medium with antibiotics was checked and did not inhibit the probiotics in the concentrations used.…”

mentioning

confidence: 99%

PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE

Ávila

Telmo

Martins

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p > 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii. KEYWORDS:Toxocariasis; Probiotics; Larvicidal action.Visceral toxocariasis is a chronic parasitic disease of worldwide distribution 12 currently regarded as a public health problem 17 . This parasitosis, commonly caused by the nematode Toxocara canis, is characterized by the migration and presence of the parasite larvae in humans. These parasites develop in the intestines of dogs, definitive hosts, that contaminate the soil with the parasite eggs. Humans and other paratenic hosts, such as mice, rats, chickens, lambs, and pigs, among others, acquire toxocariasis by ingesting T. canis eggs containing the infective-stage larvae 9 . The larvae hatch in the small intestine, where they perforate the wall and are disseminated by the systemic circulation 14 .The various clinical forms of human toxocariasis complicate treatment, making necessary the search for new alternatives of control and treatment. Although several studies have shown the benefit of the use of probiotics for the prevention and treatment of diseases, few have been conducted to evaluate the preventive effect on protozoosis 10,13,15 and helminthiasis 1,2,3 . The Saccharomyces boulardii, yeast characterized as anti-inflammatory and immunostimulant in the intestinal mucosa 3 , is also related to the reduction of parasite burden in mice infected with T. canis 1 , although the mechanism of action is unknown. Besides this fungus, the Bacillus cereus var. toyoi is a probiotic related to the stimulation of the immune response and promotes animal weight gain 7,8,11 . There is still a gap in the understanding of the mechanism of action of these and other probiotics. In the present study, we evaluated whether there is any larvicidal effect of the probiotics S. boulardii and B. cereus var. toyoi on T. canis larvae in vitro.T. canis eggs were collected directly from the uterine tubes of adult gravid female parasites obtained after the treatment of puppies with pyrantel pamoate (15 mg/kg). Afterwards, the eggs were incubated in 2% formalin solution at 28 °C, with humidity above 90% and oxygenation for 30 days. Then, the extraction of larvae was performed according to DE SAVIGNY (1975). This study was approved by the Ethics Commit...

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In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Cited by 29 publications

References 34 publications

EFFECT OF Bifidobacterium animalis ON MICE INFECTED WITH Strongyloides venezuelensis

EFFECT OF Bifidobacterium animalis ON MICE INFECTED WITH Strongyloides venezuelensis

Interactions between parasites and microbial communities in the human gut

PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE

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