Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice

Coco, Valeria Fernanda Del; Sparo, Mónica; Sidoti, Alicia; Santı́n, Mónica; Basualdo, Juan Ángel; Córdoba, María Alejandra

doi:10.1007/s00436-016-5087-1

Cited by 21 publications

(11 citation statements)

References 25 publications

(27 reference statements)

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“…This phenotype was attributed to a reduced epithelial cell turnover. The effect of probiotics on the course of cryptosporidiosis was also observed by others [22, 23]. This research uncovered a beneficial effect of Enterococcus faecalis administration to mice infected with C .…”

Section: Introductionsupporting

confidence: 80%

Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis

2019

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Based on our initial observations showing that mice consuming a probiotic product develop more severe cryptosporidiosis, we investigated the impact of other dietary interventions on the intracellular proliferation of Cryptosporidium parvum and C. tyzzeri in the mouse. Mice were orally infected with oocysts and parasite multiplication measured by quantifying fecal oocyst output. High-throughput sequencing of 16S ribosomal RNA amplicons was used to correlate oocyst output with diet and with the composition of the intestinal microbiota. On average, mice fed a diet without fiber (cellulose, pectin and inulin) developed more severe infections. As expected, a diet without fibers also significantly altered the fecal microbiota. Consistent with these observations, mice fed a prebiotic product sold for human consumption excreted significantly fewer oocysts. The fecal microbiota of mice consuming no plant polysaccharides was characterized by a lower relative abundance of Bacteroidetes bacteria. Since bacterial metabolites play an important role in the physiology of intestinal enterocytes, we hypothesize based on these observations that the impact of diet on parasite proliferation is mediated primarily by the metabolic activity of the anaerobic microbiota, specifically by the effect of certain metabolites on the host. This model is consistent with the metabolic dependence of intracellular stages of the parasite on the host cell. These observations underscore the potential of dietary interventions to alleviate the impact of cryptosporidiosis, particularly in infants at risk of recurrent enteric infections.

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Section: Introductionsupporting

confidence: 80%

Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis

2019

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“…and rotavirus are both intracellular pathogens that invade and affect the same target cells (superficial enterocytes) of the small intestine [54,55], resulting in similar lesions, it can be speculated that they occupy the same cellular or subcellular niches (i.e., receptors, signaling pathways), and/or that infection with one of them somehow interferes with the ability of the other to cause further intestinal damage and diarrhea. A study in mice experimentally infected with C. parvum and a strain of Enterococcus faecalis administered as a probiotic demonstrated that when both agents were present in the same intestinal location, the bacterium interfered with C. parvum infection [56].…”

Section: Discussionmentioning

confidence: 99%

Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study

et al. 2021

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Neonatal calf diarrhea (NCD) and mortality cause significant losses to the dairy industry. The preweaning dairy calf mortality risk in Uruguay is high (15.2%); however, causes for these losses are largely unknown. This study aimed to assess whether various pathogens were associated with NCD and death in Uruguayan dairy calves and whether these infections, diarrhea, or deaths were associated with the failure of transfer of passive immunity (FTPI). Contemporary diarrheic (n = 264,) and non-diarrheic (n = 271) 1-to 30-day-old calves from 27 farms were sampled. Feces were analyzed by antigen-capture ELISA for Cryptosporidium spp., rotavirus, bovine coronavirus, and Escherichia coli F5+, RT-PCR for bovine astrovirus (BoAstV), and bacterial cultures for Salmonella enterica. Blood/serum was analyzed by RT-PCR or antigen-capture ELISA for bovine viral diarrhea virus (BVDV). Serum of ≤ 8-day-old calves (n = 95) was assessed by refractometry to determine the concention of serum total proteins (STP) as an indicator of FTPI. Whether the sampled calves died before weaning was recorded. At least one pathogen was detected in 65.4% of the calves, and this percentage was significantly higher in diarrheic (83.7%) versus non-diarrheic (47.6%) calves. Unlike the other pathogens, Cryptosporidium spp. and rotavirus were associated with NCD. Diarrheic calves, calves infected with any of the pathogens, and calves infected with rotavirus had significantly lower concentrations of STP. Diarrheic calves had higher chances of dying before weaning than non-diarrheic calves. Diarrheic calves infected with S. enterica were at increased risk of mortality. Controlling NCD, salmonellosis, cryptosporidiosis, and rotavirus infections, and improving colostrum management practices would help to reduce calf morbi-mortality in dairy farms in Uruguay.

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“…The primary route of bacterial activity to parasitic hitting possibly through boosting gut hurdle (23)(24)(25)(26). Helpful bacteria could also increase useful microbiota numbering as lactobacillus, that suppress harmful bacteria development via contesting on connection region on the gut endothelial layer.…”

Section: Discussionmentioning

confidence: 99%

Effect of probiotic acidophilus plus against infection with secondary hydatid disease in BALB /c mice

Yousif¹,

Ali²

2020

IJVS

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The current study investigated the effect of acidophilus plus probiotic in the immune activities in mice to infestation with the cystic echinococcosis. Two dilutions of the probiotic bacteria 9*10 6 /0.1 ml, 30*10 3 /0.1 ml CFU were used, by intraperitoneally injection in the experimental animals, pre and post infections with protoscoleces of Echinococcus granulosus. Before infection remedy comprised injection by acidophilus plus twice with 72 hours interval, on the seventh day, animals were injected with protoscoleces intraperitoneally, after infection remedy implicated injection of animals with protoscoleces of Echinococcus protoscoleces first, next to 72 hours, probiotics were inoculated intraperitoneally. Many criteria were taken into consideration including, numbers, weights, diameters and percentage reduction of hydatid cysts of treated mice in contrast to the animals infested with only. The study showed a decline in cysts including their diameter, weighting, digit, accompanied by increasing the percentage reduction of hydatid cysts in treated mice, the highest percentage reduction was 98.03%, at both dilutions, 6 months post infection, and the minimum cysts number was 0.8 in comparison with the control group 39.4, with significant difference, in the same experiment. The minimum cysts weight was 0.0104 gm, 6 months post infection at the dilution 9*10 6 /0.1 ml CFU, compared with the control group 0.442 gm. The smallest cysts diameter was 0.057 mm in comparison with the control group 0.882 mm at dilution 9*10 6 /0.1 ml CFU, 6 months post infection. Acidophilus plus it may well be deduced that probiotic bacteria can be used as medicinal and remedial method against infection with hydatidosis.

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Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice

Cited by 21 publications

References 25 publications

Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis

Deprivation of dietary fiber enhances susceptibility of mice to cryptosporidiosis

Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay: a farm-matched case-control study

Effect of probiotic acidophilus plus against infection with secondary hydatid disease in BALB /c mice

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