Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

Abstract

Background Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body. Results One organism was isolated, named “L. gasseri HHuMIN D”, and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D’s KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays. Conclusion These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.

Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

“…28 L. fermentum and L. gasseri can release anti-microbial agents such as bacteriocins, 29,30 biosurfactant 31,32 and hydrogen peroxide. 33,34 In line with this, our results showed that LF23 and LG80 fermentation supernatants could significantly inhibit the growth of H. pylori and reduce H. pylori infection in vivo , indicating that they can metabolize and produce bacteriostatic substances. In the case of excluding the influence of acid, the fermentation supernatant of LAB can also significantly inhibit the growth of H. pylori , indicating that in addition to the secretion of organic acids, it can also produce other antibacterial substances.…”

In Vitro Preventive Effect and Mechanism of Action of Weissella cibaria CMU against Streptococcus mutans Biofilm Formation and Periodontal Pathogens

“…Several strains of L. paracasei have also been reported to inhibit S. mutans biofilm formation [52,53]. Mann et al [54] reported that L. gasseri inhibited the formation of biofilm by S. mutans on an orthodontic wire. Moreover, it has been reported that S. salivarius can respond to plaque formation and salivary acidity by producing dextranase and urease after colonizing the oral mucosa of humans [28].…”

Discovery of Novel Iminosugar Compounds Produced by Lactobacillus paragasseri MJM60645 and Their Anti-Biofilm Activity against Streptococcus mutans

“…Intake of fermentative food, fruits, and vegetables by a 10-month-old baby may be the reason for the existence of L. mesenteroides in the oral cavity. L. gasseri is a widely studied probiotic species with various biological functions, including oral probiotic characteristics ( 30 ). L. gasseri is known as a part of the vaginal flora, and some researchers isolated L. gasseri from breast milk ( 31 ) or saliva ( 27 ).…”