Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Zhao, Yuyang; Li, Zhipeng; Zhao, Liang; Wang, Jian; Wang, Fan; Zhang, Qi; Wang, Xiaoyu; Sang, Yue; Zhan, Jing; He, Jingjing; Li, Ning; Kang, Xiaohong; Chen, Jianguo; Wang, Ran

doi:10.1039/d2fo02034c

Cited by 11 publications

(5 citation statements)

References 48 publications

(61 reference statements)

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“…and Lactobacillus spp. have been found to significantly decrease the abundance of Helicobacter, one of the most common pathogens globally associated with gastritis and cancer, by 90% and 83%, respectively, in an infected mouse model (Zhao et al, 2022a). Moreover, Limosilactobacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Dietary Bacillus licheniformis shapes the foregut microbiota, improving nutrient digestibility and intestinal health in broiler chickens

Han

Wang

et al. 2023

Front. Microbiol.

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Bacillus licheniformis is considered a potential alternative to antibiotic growth promoters of animal growth and health. However, the effects of Bacillus licheniformis on the foregut and hindgut microbiota, and their relationships with nutrient digestion and health, in broiler chickens remain unclear. In this study, we aimed to identify the effects of Bacillus licheniformis BCG on intestinal digestion and absorption, tight junctions, inflammation, and the fore- and hind-gut microbiota. We randomly assigned 240 1-day-old male AA broilers into three treatment groups: CT (basal diet), BCG1 (basal diet + 1.0 × 108 CFU/kg B. licheniformis BCG), and BCG2 (basal diet + 1.0 × 109 CFU/kg B. licheniformis BCG). On day 42, the jejunal and ileal chyme and mucosa were subjected to analysis of digestive enzyme activity, nutrient transporters, tight junctions, and signaling molecules associated with inflammation. The ileal and cecal chyme were subjected to microbiota analysis. Compared with the CT group, the B. licheniformis BCG group showed significantly greater jejunal and ileal α-amylase, maltase, and sucrase activity; moreover, the α-amylase activity in the BCG2 group was higher than that in the BCG1 group (P < 0.05). The transcript abundance of FABP-1 and FATP-1 in the BCG2 group was significantly greater than that in the CT and BCG1 groups, and the GLUT-2 and LAT-1 relative mRNA levels were greater in the BCG2 group than the CT group (P < 0.05). Dietary B. licheniformis BCG resulted in significantly higher ileal occludin, and lower IL-8 and TLR-4 mRNA levels than observed in the CT group (P < 0.05). B. licheniformis BCG supplementation significantly decreased bacterial community richness and diversity in the ileum (P < 0.05). Dietary B. licheniformis BCG shaped the ileac microbiota by increasing the prevalence of f_Sphingomonadaceae, Sphingomonas, and Limosilactobacillus, and contributed to nutrient digestion and absorption; moreover, it enhanced the intestinal barrier by increasing the prevalence of f_Lactobacillaceae, Lactobacillus, and Limosilactobacillus. Dietary B. licheniformis BCG decreased microbial community diversity by diminishing Desulfovibrio, Alistipes, Campylobacter, Vibrio, Streptococcus, and Escherichia coli-Shigella levels, and down-regulating inflammatory associated molecule expression. Therefore, dietary B. licheniformis BCG contributed to digestion and absorption of nutrients, enhanced the intestinal physical barrier, and decreased intestinal inflammation in broilers by decreasing microbial diversity and optimizing the microbiota structure.

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Section: Discussionmentioning

confidence: 99%

Dietary Bacillus licheniformis shapes the foregut microbiota, improving nutrient digestibility and intestinal health in broiler chickens

Han

Wang

et al. 2023

Front. Microbiol.

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“…60 Furthermore, persistent infection with H. pylori can induce the production of ROS and pro-inflammatory cytokines, including IL-1β, IL-6, IL-8, and TNF-α, which are involved in the augmented mucosal damage and development of mucosa-associated lymphoid tissue (MALT) lymphoma and gastric adenocarcinoma. 62 Substantial evidence has reported that LAB can impair the pathogenic virulence of invading pathogens, decreasing inflammatory cytokine production as well as alleviating mucosal damage 20,21,87,88 Napoli et al reported that EFV12 derived from Lactobacillus gasseri SF1109 could interfere with mitogen-activated protein kinase signaling pathways by binding lipopolysaccharides and preventing lipopolysaccharide (LPS) attachment with Toll-like receptor 4. 89 Furthermore, supplementation with various concentrations of PLA in basal diet enhanced the level of IgY (immunoglobulin Y) in broiler chickens.…”

Section: Discussionmentioning

confidence: 99%

Phenyl lactic acid alleviatesHelicobacter pyloriinfection in C57BL/6 mice

Cui

Zhou

et al. 2023

Food Funct.

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“…Lactobacillus and Bifidobacterium are the two most common species found in probiotic products such as yogurt ( Xiong, 2018 ; Ye et al, 2020 ). In the past, the positive effects of dairy products such as yogurt on digestion were proven ( Whiteside et al, 2021 ; Zhao et al, 2022 ). Other species accessible on the market include Bacillus spp., Enterococci , E. coli , Weissella spp., and Saccharomyces .…”

Section: Alternative Therapiesmentioning

confidence: 99%

A review for non-antibiotic treatment of Helicobacter pylori: new insight

Shadvar,

Akrami,

Mousavi Sagharchi

et al. 2024

Front. Microbiol.

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Gastric ulcers and gastric cancer are brought on by the Helicobacter pylori bacteria, which colonizes under the stomach mucous membrane. Different medication regimens are used to remove it, but the illness returns and becomes more resistant, which lowers the treatment rates. Additionally, this bacterium now exhibits a skyrocketing level of multi-drug resistance, necessitating recurrent therapeutic treatments. The negative effects of synthetic medications in comparison to conventional therapies are another significant factor in favor of non-pharmacological therapy. The most significant side effects of popular anti-gastric ulcer medications include nausea, vomiting, and diarrhea. Stomach ulcers have previously been treated with herbal remedies and complementary treatments like probiotics. When probiotics are ingested, the host experiences several advantages that may be brought about by altering the bacterial flora in the digestive system. Additionally, stronger-acting chemical compounds and plant extracts can be employed to treat patients. In this article, we look at the substances and medications that are utilized in place of synthetic stomach ulcer-curing treatments.

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Two novel lactic acid bacteria, Limosilactobacillus fermentum MN-LF23 and Lactobacillus gasseri MN-LG80, inhibited Helicobacter pylori infection in C57BL/6 mice

Abstract: Helicobacter pylori (H. pylori) is one of the most prevalent pathogens globally, and long-term infection causes various gastrointestinal diseases such as gastritis and even cancer. In the present study, we...

Cited by 11 publications

References 48 publications

Dietary Bacillus licheniformis shapes the foregut microbiota, improving nutrient digestibility and intestinal health in broiler chickens

Dietary Bacillus licheniformis shapes the foregut microbiota, improving nutrient digestibility and intestinal health in broiler chickens

Phenyl lactic acid alleviatesHelicobacter pyloriinfection in C57BL/6 mice

A review for non-antibiotic treatment of Helicobacter pylori: new insight

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