Discovery of Novel Iminosugar Compounds Produced by
            <i>Lactobacillus paragasseri</i>
            MJM60645 and Their Anti-Biofilm Activity against Streptococcus mutans

Gu, Mingkun; Cheng, Jinhua; Lee, Yeong‐Geun; Cho, Joo-Hyung; Suh, Joo‐Won

doi:10.1128/spectrum.01122-22

Cited by 7 publications

(6 citation statements)

References 59 publications

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“…The underlying element might relate to the direct antagonistic activity of organic acid or the supportive action of acidic conditions to activate bacteriocin-like compounds that are active at low pH. 6,30,31,32 The ability of the bacterium CFS isolated from fermented foods to produce powerful antibiotic substances that can break the biofilm to battle S. mutans MTCC-890 colonization and biofilm formation was demonstrated. The cell wall production, cell metabolism, and cell attachments may all be impacted by the metabolites produced by the isolated probiotics.…”

Section: Discussionmentioning

confidence: 99%

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Pallavi

Barik

Sahoo

et al. 2023

Biotech and App Biochem

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Streptococcus mutans plays a major role in biofilm formation and pathogenic bacterial adhesion. Here we investigated the abilities of our isolates from diverse conventional sources to characterize the beneficial bacteria for inhibition of S. mutans. Enterobacter cloacae PS‐74, a beneficial bacteria isolated from yoghurt, is gram negative, rod shaped, and resistant to acid, bile salt, and amylase. PS‐74 cell‐free supernatants (CFS) demonstrated highest zone of inhibition of 29 ± 1.7 mm. Further, the minimum inhibitory concentration (MIC) value of CFS PS‐74 was recorded to be 10 μL and its minimum bactericidal concentration (MBC) value was found to be 15μL which led to 99.9% log reduction of S. mutans. Moreover, the biofilm formation was reduced by 84.91% at MIC15 of CFS PS‐74 which alleviate the dental caries formation by S. mutans. This is the first report on E. cloacae PS‐74, which was studied for its probiotic properties to inhibit S. mutans MTCC‐890 due to the production of organic acids and employed in oral treatment.

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Section: Discussionmentioning

confidence: 99%

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Pallavi

Barik

Sahoo

et al. 2023

Biotech and App Biochem

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“…Wasfi R et al [ 43 ] reported that biosurfactant produced by L. acidophilus can inhibit the biofilm formation of S. mutans . Gu, et al [ 44 ] discovered novel iminosugar compounds that can interfere with the biofilm formation of S. mutans . It is necessary to explore the active compound in further study.…”

Section: Discussionmentioning

confidence: 99%

Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition

Cho

Suh

et al. 2022

Journal of Oral Microbiology

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Streptococcus mutans is known as a contributor to dental caries. In this work, Lactobacillus pentosus MJM60383 was selected for its strong antagonistic activity against S. mutans and was characterized by good oral probiotic properties including lysozyme tolerance, adhesive ability to oral cells, good aggregation (auto-aggregation, co-aggregation) ability, hydrogen peroxide production and inhibition of biofilm formation of S. mutans . L. pentosus MJM60383 also exhibited safety as a probiotic characterized by no hemolytic activity, no D-lactate production, no biogenic amine production, and susceptibility to antibiotics. Furthermore, the biofilm formation of S. mutans was also significantly inhibited by the supernatant of L. pentosus MJM60383. An anti-biofilm mechanism study revealed that sucrose decomposition and the production of water-insoluble exopolysaccharides by S. mutans were inhibited by the treatment with L. pentosus MJM60383 supernatant. Real-time PCR analysis indicated that the supernatant of L. pentosus MJM60383 significantly inhibited the mRNA expression of S. mutans glycosyltransferases, which synthesize glucan to construct biofilm architecture and mediate bacterial adherence. Our study demonstrated L. pentosus MJM60383 as a potential oral probiotic and revealed its anti-biofilm mechanism.

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“…The dry leaf of IXD was crushed and extracted with 70% ethanol at the ratio of 1.38% at 85 °C for 36 h. Then the filtrate was concentrated by using a rotary evaporator (EYELA, Japan) under vacuum. Lactobacillus strain MJM60645 was isolated from infant’s oral cavity as described in the previous study 26 . 16S rDNA sequence of MJM60645 showed 99% similarity with Lactobacillus paragasseri .…”

Section: Methodsmentioning

confidence: 99%

Ixeris dentata and Lactobacillus gasseri media protect against periodontitis through Nrf2-HO-1 signalling pathway

Lee

Kim

et al. 2023

Sci Rep

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Periodontitis is an infectious inflammation in the gums characterized by loss of periodontal ligaments and alveolar bone. Its persistent inflammation could result in tooth loss and other health issues. Ixeris dentata (IXD) and Lactobacillus gasseri media (LGM) demonstrated strong antioxidant activity, which may prevent oxidative and inflammatory periodontitis. Here, IXD and LGM extracts were investigated for antioxidative activity against oral discomfort and evaluated for their synergistic effect against oxidative and inflammatory periodontitis in a mouse model. IXD/LGM suppressed pro-inflammatory cytokines like interleukin (IL)-1β, IL-6, and TNF-α. Additionally, it reduced pro-inflammatory mediators, nitric oxide, iNOS (inducible nitric oxide synthase), and COX-2 (cyclooxygenase-2) and enhanced AKT, Nrf2, and HO-1 activation. Similarly, IXD/LGM treatment elevated osteogenic proteins and mRNAs; alkaline phosphatase, collagen type 1 (COL1), osteopontin (OPN), and runt-related transcription factor 2 (RUNX2). Hematoxylin and Eosin (H&E) staining and micro-CT analysis confirm the positive impact of IXD/LGM on the periodontal structure and its associated inflammation. These findings demonstrate that IXD/LGM inhibits oxidative stress, periodontal inflammation, and its resultant alveolar bone loss in which Akt (also known as protein kinase B)-nuclear factor-erythroid 2-related factor 2 (Nrf2)-hemoxygenase-1 (HO-1) signaling is involved. Thus, IXD/LGM is a potential candidate against oxidative/inflammatory stress-associated periodontitis.

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Discovery of Novel Iminosugar Compounds Produced by Lactobacillus paragasseri MJM60645 and Their Anti-Biofilm Activity against Streptococcus mutans

Cited by 7 publications

References 59 publications

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition

Ixeris dentata and Lactobacillus gasseri media protect against periodontitis through Nrf2-HO-1 signalling pathway

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