Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition

Abstract

Streptococcus mutans is known as a contributor to dental caries. In this work, Lactobacillus pentosus MJM60383 was selected for its strong antagonistic activity against S. mutans and was characterized by good oral probiotic properties including lysozyme tolerance, adhesive ability to oral cells, good aggregation (auto-aggregation, co-aggregation) ability, hydrogen peroxide production and inhibition of biofilm formation of S. mutans . L. pentosus MJM60383 also exhibited safety as a probiotic characterized by no hemolytic activity, no D-lactate production, no biogenic amine production, and susceptibility to antibiotics. Furthermore, the biofilm formation of S. mutans was also significantly inhibited by the supernatant of L. pentosus MJM60383. An anti-biofilm mechanism study revealed that sucrose decomposition and the production of water-insoluble exopolysaccharides by S. mutans were inhibited by the treatment with L. pentosus MJM60383 supernatant. Real-time PCR analysis indicated that the supernatant of L. pentosus MJM60383 significantly inhibited the mRNA expression of S. mutans glycosyltransferases, which synthesize glucan to construct biofilm architecture and mediate bacterial adherence. Our study demonstrated L. pentosus MJM60383 as a potential oral probiotic and revealed its anti-biofilm mechanism.

Effects of Lactobacillus casei and Aggregatibactor actinomycetemcomitans against Streptococcus mutans according to the Concentration of Sucrose

“…Our study first suggested that the growth and biofilm formation by A. actinomycetemcomitans were affected by sucrose. In previous in vitro studies, L. plantarum 9) , L. acidophilus 12) and L. reuteri 25) inhibited the biofilm formation by S. mutans. L. fermentum 26) and L. reuteri 25) reduced the growth of S.…”

“…Therefore, it can be suggested that the antibacterial and anti-biofilm activities of L. casei against S. mutans are due to other anti-microbial mechanisms than the acidic environment caused by the production of organic acid and hydrogen peroxide by L. casei. The inhibitory effect of L. casei against S. mutans may be due to the production of bacteriocin or bacteriocin-like polypeptides, which destroy the cell membrane of S. mutans 9,12) and production of biosurfactant 30) , as well as the auto-aggregation and co-aggregation ability to compete with S. mutans for nutrition or adhesive surface 31,32) and the ability of anti-biofilm formation through the inhibition of sucrose decomposition of S. mutans 9) .…”

“…Members of genus Lactobacillus colonize the oral tissues and coagulates with other bacteria adjacent to it, forming a biofilm 9) , further acidifying the surrounding environment and accelerating dental caries. They are known to be involved in advanced dental caries 10) .…”

Anti-Oral Microbial Effect of Ethanol Extract of Angelica gigas Nakai

“…S. mutans causes dental caries and forms biofilms on the tooth surface 17) . L. casei causes advanced dental caries 14) , and colonizes oral tissues to form biofilm 18) . A. actinomycetemcomitans causes aggressive periodontal disease and colonizes dental plaque 19,20) .…”

Alleviating effect of Lactobacillus pentosus CQZC01 probiotic strain on antibiotic-induced diarrhea in mice

“…These microorganisms are familiar with and adapted to the human body's environment, so under appropriate usage conditions, probiotic formulations will not cause adverse reactions in the human body (11). Some strains of L. pentosus are derived from naturally fermented foods and possess bioactive effects, making them safe and reliable for potential development as pharmaceutical probiotic formulations (12).…”