Oral Microbiota in Infants Fed a Formula Supplemented with Bovine Milk Fat Globule Membranes - A Randomized Controlled Trial

Abstract

BackgroundIn a recent study, supplementation of infant formula with milk fat globule membranes (MFGM) decreased the incidence of otitis media in infants <6 months of age.ObjectivesThe aim of the present study was to characterize the oral microbiota in infants fed MFGM-supplemented formula and compare it to that of infants fed standard formula or breast milk.MethodsIn a prospective double-blinded randomized controlled trial, exclusively formula-fed infants <2 months of age were randomized to be fed experimental formula (EF, n = 80) with reduced energy and protein and supplemented with a bovine MFGM concentrate, or standard formula (SF, n = 80) until 6 months of age. A breast-fed reference (BFR, n = 80) group was also recruited. The oral microbiota was analyzed at 4 (n = 124) and 12 (n = 166) months of age using Illumina MiSeq multiplex sequencing and taxonomic resolution against the HOMD 16S rDNA database of oral bacteria.ResultsSpecies richness in the oral samples did not differ between the EF and SF groups, but partial least square modeling identified a few taxa that were significantly associated with being in either group, e.g. lower level of Moraxella catarrhalis in the EF group. Infants in the BFR group had significantly lower species richness at 4 months of age and their microbiota pattern differed markedly from the formula-fed groups.ConclusionsSupplementation of infant formula with MFGM yielded moderate effects on the oral microbiome. Moraxella catarrhalis was less prevalent in infants fed EF than in those fed SF and may be associated with the decrease in otitis media seen in the same group.

Influence of delivery and feeding mode in oral fungi colonization – a systematic review

“…Firstly, our results from the literature review show that delivery mode might be associated with a higher carriage of oral fungi in young age (Figure 2) and, on the other hand, in the light of our results, the type of diet of the infant (formula-fed or breast-fed infants) does not seem to have impact on oral yeast carriage, although it is currently recognized that fungi are present in human breastmilk [57]. These results may be explained by the antimicrobial factors of the human milk influencing the oral ecology [58], although further studies are necessary.…”

HPV infection and bacterial microbiota in breast milk and infant oral mucosa

“…While factors including prematurity, maternal intrapartum antibiotics, delivery mode and formula feeding all affect infant oral microbiota composition[ 30 – 32 ], the microbes from the maternal mouth as well as the mammary areola and breast milk are the primary sources of bacteria for the infant mouth[ 5 , 22 ]. In our study population, we detected a shared core of eight bacterial genera between breast milk and infant oral mucosa microbiota.…”

Modulation of miRNA expression in aged rat hippocampus by buttermilk and krill oil

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