Oral delivery of fish oil in oil-in-water nanoemulsion: development, colloidal stability and modulatory effect on in vivo inflammatory induction in mice

Santos, Débora Silva; Morais, José Athayde Vasconcelos; Vanderlei, Ísis A.C.; Santos, Alexandre Soares dos; Azevedo, Ricardo Bentes; Muehlmann, Luís Alexandre; Júnior, Osmindo Rodrigues Pires; Mortari, Márcia Renata; Silva, Jaqueline da; Silva, S. W. da; Longo, João Paulo Figueiró

doi:10.1016/j.biopha.2020.110980

Cited by 19 publications

(12 citation statements)

References 51 publications

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“…Researchers are also developing more precise ( Adesnik and Abdeladim, 2021 ) and less invasive optogenetic devices, such as SOUL ( Gong et al, 2020 ). At the same time, optogenetics has strong compatibility, it can be used to study a variety of diseases, such as diabetes ( Li et al, 2021 ), inflammation ( Baumschlager and Khammash, 2021 ; Bhat et al, 2021 ; Dos Santos et al, 2021 ; Jamaluddin et al, 2021 ; Michoud et al, 2021 ; Santos et al, 2021 ; Senok et al, 2021 ), tumors ( Kim et al, 2017 ; Adampourezare et al, 2021 ; Esmaeili et al, 2022 ), depression ( Hare et al, 2019 ), epilepsy ( Zhang and Wang, 2021 ) and so on. And in 2020, 45 laboratories around the world integrated all optogenetics resources and create an optogenetics experimental database ( Tremblay et al, 2020 ).…”

Section: Conclusion Challenges and Perspectivesmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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Optogenetic is a technique that combines optics and genetics to control specific neurons. This technique usually uses adenoviruses that encode photosensitive protein. The adenovirus may concentrate in a specific neural region. By shining light on the target nerve region, the photosensitive protein encoded by the adenovirus is controlled. Photosensitive proteins controlled by light can selectively allow ions inside and outside the cell membrane to pass through, resulting in inhibition or activation effects. Due to the high precision and minimally invasive, optogenetics has achieved good results in many fields, especially in the field of neuron functions and neural circuits. Significant advances have also been made in the study of many clinical diseases. This review focuses on the research of optogenetics in the field of neurobiology. These include how to use optogenetics to control nerve cells, study neural circuits, and treat diseases by changing the state of neurons. We hoped that this review will give a comprehensive understanding of the progress of optogenetics in the field of neurobiology.

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Section: Conclusion Challenges and Perspectivesmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

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“…Fish oil samples were obtained from fish oil capsules (Naturalis Nutrição & Farma, Ltd., Curitiba, Brazil; 1 g) containing a mixture of DHA and EPA (DHA 500 mg; EPA 100 mg). DHA was used as a fish oil biomarker and measured to confirm the amount of DHA in the fish oil samples, as described by Santos et al [ 16 ]. These results, as well as the nanoscopic characterization (mean hydrodynamic diameter of 156 nm; PDI of 0.34; and zeta potential of −16.9 mW), were confirmed by us in the present article.…”

Section: Methodsmentioning

confidence: 99%

“…Pretreatment was carried out for 21 days, using the gastric gavage method, with the experimental groups: (1) PBS, (2) SO, (3) FO, and (4) NEW3. A total dose of 100 mg/kg, diluted in 200 µL, was used, considering the composition and dose studied by our research group [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

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Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice

et al. 2022

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Diets rich in omega-3 or -6 fatty acids will produce different profiles for cell membranes phospholipid constitutions. Omegas 3 and 6 are part of the diet and can modulate the inflammatory profile. We evaluated the effects of the oral absorption of fish oil, when associated with a lipid nanoemulsion in an experimental pulmonary inflammatory model. Pulmonary fibrosis is a disease associated with excessive extracellular matrix deposition. We determined to investigate the morphophysiological mechanisms in mice that were pretreated after induction with bleomycin (BLM). The pretreatment was for 21 days with saline solution, sunflower oil (SO), fish oil (FO), and fish oil nanoemulsion (NEW3). The animals received a daily dose of 50 mg/Kg of docosahexaenoic acid DHA and 10 mg/Kg eicosapentaenoic (EPA) (100 mg/Kg), represented by a daily dose of 40 µL of NEW3. The blank group was treated with the same amount daily (40 µL) during the 21 days of pretreatment. The animals were treated with SO and FO, 100 mg/Kg (containing 58 mg/Kg of polyunsaturated fats/higher% linoleic acid) and 100 mg/Kg (50 mg/Kg of DHA and 10 mg/Kg EPA), respectively. A single dose of 5 mg/mL (50 μL) bleomycin sulfate, by the intratracheal surgical method in BALB/cAnNTac (BALB/c). NEW3 significantly reduced fibrotic progression, which can be evidenced by the protection from loss of body mass, increase in respiratory incursions per minute, decreased spacing of alveolar septa, decreased severity of fibrosis, and changes in the respiratory system. NEW3 attenuated the inflammatory changes developed in the experimental model of pulmonary fibrosis, while group SO showed a significant increase in inflammatory changes. This concluded that the presented results demonstrated that is possible to positively modulate the immune and inflamamtory response to an external agressor, by changing the nutitional intake of specific fatty acids, such as omega-3 placed in fish oil. Moreover, these benefits can be improved by the nanoencapsulation of fish oil in lipid nanoemulsions.

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“…Valores elevados de viabilidade celular de macrófagos em relação aos grupos controle são uma evidência importante que demonstra a capacidade da formulação de se associar a membranas biológicas (Santos et al, 2021).…”

Section: Efeito Citotóxico Em Macrófagos Raw 2647 Da Solução Filmogênicaunclassified

Desenvolvimento e caracterização de filmes curativos à base de mesocarpo de Attalea speciosa Mart. ex Spreng e extrato glicólico de Aloe vera (L.) Burm. f.

Araruna

Pereira

Brito

et al. 2021

RSD

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O amido de mesocarpo de babaçu (Attalea speciosa Mart. ex Spreng) e a Aloe vera (Aloe vera (L.) Burm. f.) são materiais naturais com vasta aplicação nas áreas de saúde. Neste trabalho de pesquisa foram preparados e caracterizados filmes casting compostos por amido de mesocarpo de babaçu suplementados com Aloe vera nas proporções de 0%, 5%, 8% e 10% observando características físicas de espessura, umidade, solubilidades em água, permeabilidade ao vapor de água e propriedades mecânicas. Além disso, foram avaliadas a citotoxicidade da solução filmogênica em macrófagos murinos da linhagem RAW 264.7 e células de hemácias humanas. Os resultados mostram que os filmes desenvolvidos têm superfície lisa, boa maleabilidade e são autossustentados. O teor de umidade, solubilidade e permeabilidade ao vapor de água aumentaram de acordo com o aumento da concentração de Aloe vera, para as propriedades mecânicas não houve diferença significativa (p>0,05) entre as formulações testadas. A solução filmogênica apresentou efeito citotóxico considerado seguro para os macrófagos e hemácias humanas. Essas características demonstram um bom potencial para cobertura de feridas e reparação tecidual.

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Oral delivery of fish oil in oil-in-water nanoemulsion: development, colloidal stability and modulatory effect on in vivo inflammatory induction in mice

Cited by 19 publications

References 51 publications

The Roles of Optogenetics and Technology in Neurobiology: A Review

The Roles of Optogenetics and Technology in Neurobiology: A Review

Fish Oil Nanoemulsion Supplementation Attenuates Bleomycin-Induced Pulmonary Fibrosis BALB/c Mice

Desenvolvimento e caracterização de filmes curativos à base de mesocarpo de Attalea speciosa Mart. ex Spreng e extrato glicólico de Aloe vera (L.) Burm. f.

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