Oral chondroitin sulfate and prebiotics for the treatment of canine Inflammatory Bowel Disease: a randomized, controlled clinical trial

Abstract

Background: Canine inflammatory bowel disease (IBD) is a chronic enteropathy of unknown etiology, although microbiome dysbiosis, genetic susceptibility, and dietary and/or environmental factors are hypothesized to be involved in its pathogenesis. Since some of the current therapies are associated with severe side effects, novel therapeutic modalities are needed. A new oral supplement for long-term management of canine IBD containing chondroitin sulfate (CS) and prebiotics (resistant starch, β-glucans and mannaoligosaccharides) was developed to target intestinal inflammation and oxidative stress, and restore normobiosis, without exhibiting any side effects. This double-blinded, randomized, placebo-controlled trial in dogs with IBD aims to evaluate the effects of 180 days administration of this supplement together with a hydrolyzed diet on clinical signs, intestinal histology, gut microbiota, and serum biomarkers of inflammation and oxidative stress. Results: Twenty-seven client-owned biopsy-confirmed IBD dogs were included in the study, switched to the same hydrolyzed diet and classified into one of two groups: supplement and placebo. Initially, there were no significant differences between groups (p > 0.05) for any of the studied parameters. Final data analysis (supplement: n = 9; placebo: n = 10) showed a significant decrease in canine IBD activity index (CIBDAI) score in both groups after treatment (p < 0.001). After treatment, a significant decrease (1.53-fold; p < 0.01) in histologic score was seen only in the supplement group. When groups were compared, the supplement group showed significantly higher serum cholesterol (p < 0.05) and paraoxonase-1 (PON1) levels after 60 days of treatment (p < 0.01), and the placebo group showed significantly reduced serum total antioxidant capacity (TAC) levels after 120 days (p < 0.05). No significant differences were found between groups at any time point for CIBDAI, WSAVA histologic score and fecal microbiota evaluated by PCR-restriction fragment length polymorphism (PCR-RFLP). No side effects were reported in any group. Conclusions: The combined administration of the supplement with hydrolyzed diet over 180 days was safe and induced improvements in selected serum biomarkers, possibly suggesting a reduction in disease activity. This study was likely underpowered, therefore larger studies are warranted in order to demonstrate a supplemental effect to dietary treatment of this supplement on intestinal histology and CIBDAI.

Molecular effects of chondroguard in osteoarthritis and herniated discs

“…Кроме того, показана перспективность применения субстанции CS-BIOACTIVE© Биоиберика С.А.У. (Испания) при различных воспалитель-ных процессах, которые коморбидны заболеваниям, сопро-вождающимся поражением хрящевой ткани [67][68][69][70][71].…”

Chondroitin Sulfate Elicits Systemic Pathogenesis In Mice By Interfering With Gut Microbiota Homeostasis

“…It was also demonstrated that a combined therapy of CS with glucosamine sulfate or glucosamine hydrochloride does not improve joint damage in a rabbit model of knee OA (Roman-Blas et al, 2017a), and CS plus glucosamine sulfate shows no superiority over placebo in a randomized, double-blind and placebo-controlled clinical trial in patients with knee OA (Roman-Blas et al, 2017b). In contrast, an anti-inflammatory effect of CS was filed in amelioration of some inflammatory diseases, at least including inflammatory bowel disease (IBD) (Segarra et al, 2016;Holleran et al, 2017).…”

Inflammatory Bowel Disease and Current Treatment Options in Dogs

“…Prebiotics could reduce intestinal inflammation, oxidative stress and gut symbiosis (Segarra et al, 2016).…”

“…For instance, Segarra et al (2016) used a dietary supplement containing CS and prebiotics in dogs with IBD over 180 days. The study showed that long-term oral administration of the combined administration of the supplement with hydrolyzed diet was safe and beneficial for treatment of IBD in dogs.…”