Optofluidics for handling and analysis of single living cells

Perozziello, Gerardo; Candeloro, Patrizio; Coluccio, Maria Laura; Fabrizio, Enzo M. Di

doi:10.1515/optof-2017-0004

Cited by 3 publications

(3 citation statements)

References 33 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it can as yet not be used as the only technique for any kind of diagnostics, and is either combined with other tests to create assays [63,66], or needs massive automation and robotics [156] or artificial intelligence to arrive at a diagnosis [156,157]. The usage of optical traps for single cell diagnosis is mostly limited to optical stretching [158], cell-sorting [66,154,[159][160][161][162], and measuring of refractive indices [140,[162][163][164]. Especially in combination with automatization, optical traps for single cell diagnostics have a great potential, but currently it is among the more expensive and less-performing technologies.…”

Section: Figurementioning

confidence: 99%

“…Works intracellularly Relies on differences in refractive indices (e.g. DNA-rich parts like the nucleus) The application of optical tweezers for the handling of single cells and for diagnostics on the single cell level has recently been discussed a lot [138,141,154,155]. However, it can as yet not be used as the only technique for any kind of diagnostics, and is either combined with other tests to create assays [63,66], or needs massive automation and robotics [156] or artificial intelligence to arrive at a diagnosis [156,157].…”

Section: Contact-free Micromanipulation Needs Electricity and Lasers mentioning

confidence: 99%

See 1 more Smart Citation

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

View full text Add to dashboard Cite

In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

show abstract

Section: Figurementioning

confidence: 99%

Section: Contact-free Micromanipulation Needs Electricity and Lasers mentioning

confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

View full text Add to dashboard Cite

show abstract

“…Localized surface plasmon (LSP) and surface plasmon polariton (SPP) have been developed to manipulate fluid convection, living cells, DNA, and proteins. [9][10][11][12][13] For example, the photothermal effect caused by LSPs of Au NPs' array in optofluidics was investigated by Miao et al [12] Such LSP energy-induced optofluidic mixing could obtain high optical-to-thermal energy conversion efficiency. Min et al showed the optofluidic trapping and manipulation of metallic particles by the excitation of SPP on a thin layer of the gold film.…”

mentioning

confidence: 99%

Manipulation of Fluid Convection by Surface Lattice Resonance

Jing

Peng

Movsesyan

et al. 2022

Advanced Optical Materials

View full text Add to dashboard Cite

reported that the fluid flow control can be achieved at the scale of optical wavelengths, which led to the development of applications in biochemical assaybased microfluidics and nanophysics. [1,2] However, the diffraction limit of light restricts optofluidic applications at the molecular level. Additionally, high-intensity optical pulses cause undesirable photo bleaching or photoinduced damage to biomedical molecules in an optofluidic system. Surface plasmons are the collective excitation of free electrons in metals, allowing breaking the diffraction limit for the localization of light into subwavelength dimensions, enabling strong field enhancements and light-matter interactions. [3] In particular, photothermal effects in plasmonic nanoparticles (NPs) can be enhanced via enhanced light absorption, generating heat via nonradiative decay channel. [4][5][6][7][8] One can thereby manipulate fluid flows; and control suspended objects at the subwavelength scale by using plasmonics. Localized surface plasmon (LSP) and surface plasmon polariton (SPP) have been developed to manipulate fluid convection, living cells, DNA, and proteins. [9][10][11][12][13] For example, the photothermal effect caused by LSPs of Au NPs' array in optofluidics was investigated by Miao et al. [12] Such LSP energy-induced optofluidic mixing could obtain high optical-to-thermal energy conversion efficiency. Min et al. showed the optofluidic trapping and manipulation of metallic particles by the excitation of SPP on a thin layer of the gold film. [13] Such a highly confined SPP field, strongly interacting with metallic particles, forms gradient and scattering forces required for the electromagnetic trapping act. Plasmonics can also find its application in high-sensitive biomolecular binding events. For instance, plasmonic nanohole arrays were used as substrates for label-free detection. [14] However, the practical application of these LSP-or SPP-based metasurfaces is limited in plasmonic optofluidics due to the dephasing and broad bandwidth. The high-quality (Q)-factor of the plasmonic surface lattice resonance (SLR) can alleviate the problem. The interference between the LSP and the diffractive behavior of the periodic metallic nanostructures characterizes the SLR. The SLRs are well known for overcoming the damping of LSPs and minimizing scattering losses from gratings, resulting in very narrow bandwidth. Considering that SLRs for noble metal lattices have very high Q-factors with respect to LSPs, the plasmon dephasing time is several orders of magnitude longer for SLRs, [15] allowing long-life light trapping.The capability of plasmonic metasurfaces (PMs) under illumination to generate heat and induce fluid convection is a promising building approach for optofluidic applications. However, the low quality (Q)-factor in PMs introduced into optofluidic applications remains a challenging problem. In this paper, a PM optofluidic platform based on surface lattice resonance (SLR) with a high Q-factor is proposed. Numerical results demonstrate that SLR-...

show abstract