Optofluidic Tomography on a Chip

Isikman, Serhan O.; Bishara, Waheb; Zhu, Hongying; Özcan, Aydogan

doi:10.1063/1.3548564

Cited by 71 publications

(66 citation statements)

References 20 publications

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“…Holographic microscopy records the interference between a reference wave of light and the wave scattered from the object under investigation. Of particular recent interest is lensfree holographic computational microscopy, where no imaging lenses are necessary [55–59]. In this method, interferograms are recorded directly on a CCD or CMOS imaging chip, and images of the original objects are reconstructed computationally.…”

Section: Applications Of Self-assembly To Nano-imagingmentioning

confidence: 99%

Nano-imaging enabled via self-assembly

McLeod

Ozcan

2014

Nano Today

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SUMMARY Imaging object details with length scales below approximately 200 nm has been historically difficult for conventional microscope objective lenses because of their inability to resolve features smaller than one-half the optical wavelength. Here we review some of the recent approaches to surpass this limit by harnessing self-assembly as a fabrication mechanism. Self-assembly can be used to form individual nano- and micro-lenses, as well as to form extended arrays of such lenses. These lenses have been shown to enable imaging with resolutions as small as 50 nm half-pitch using visible light, which is well below the Abbe diffraction limit. Furthermore, self-assembled nano-lenses can be used to boost contrast and signal levels from small nano-particles, enabling them to be detected relative to background noise. Finally, alternative nano-imaging applications of self-assembly are discussed, including three-dimensional imaging, enhanced coupling from light-emitting diodes, and the fabrication of contrast agents such as quantum dots and nanoparticles.

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Section: Applications Of Self-assembly To Nano-imagingmentioning

confidence: 99%

Nano-imaging enabled via self-assembly

McLeod

Ozcan

2014

Nano Today

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“…Therefore, despite their limited temporal coherence, LEDs are highly desired for field-portable pixel-super resolution microscopy24313242. In addition to significantly reduced speckle and multiple reflection interference noise, such broader band sources also improve the light efficiency and allow shorter image acquisition times as well as longer object-to-sensor distances.…”

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confidence: 99%

Spectral Demultiplexing in Holographic and Fluorescent On-chip Microscopy

Şencan

Coşkun

Sikora

et al. 2014

Sci Rep

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Lensfree on-chip imaging and sensing platforms provide compact and cost-effective designs for various telemedicine and lab-on-a-chip applications. In this work, we demonstrate computational solutions for some of the challenges associated with (i) the use of broadband, partially-coherent illumination sources for on-chip holographic imaging, and (ii) multicolor detection for lensfree fluorescent on-chip microscopy. Specifically, we introduce spectral demultiplexing approaches that aim to digitally narrow the spectral content of broadband illumination sources (such as wide-band light emitting diodes or even sunlight) to improve spatial resolution in holographic on-chip microscopy. We also demonstrate the application of such spectral demultiplexing approaches for wide-field imaging of multicolor fluorescent objects on a chip. These computational approaches can be used to replace e.g., thin-film interference filters, gratings or other optical components used for spectral multiplexing/demultiplexing, which can form a desirable solution for cost-effective and compact wide-field microscopy and sensing needs on a chip.

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“…Computational imaging without lenses has been recently emerging as a new biomedical imaging technique123456789101112131415161718192021. Such lensfree imaging modalities record transmitted, scattered, or emitted photons from objects that are placed directly on or only <2–3 millimeters away from the active area of a sensor-array such as a CMOS (Complementary Metal-Oxide-Semiconductor) or a CCD (Charge-Coupled Device) chip.…”

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confidence: 99%

“…Due to this “on-chip” imaging architecture, the imaging field-of-view (FOV) equals the active area of each sensor-array, and therefore it can routinely reach e.g., 20–30 mm 2 using a standard CMOS imager or ~10–20 cm 2 using a large format CCD chip. In its holographic implementation1678919, in addition to FOV, the depth-of-field (DOF) is also significantly enhanced (e.g., ~1–5 mm), which permits rapid imaging of large specimen volumes of for example >20–2,000 μL on a chip. This throughput advantage, when combined with the compactness and cost-effectiveness of its imaging architecture, which can also be integrated with microfluidic channels789, or wireless devices such as cell phones10, makes lensfree holographic on-chip imaging a promising modality especially for biomedical imaging and diagnostic needs in resource-limited settings as well as for telemedicine applications.…”

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confidence: 99%

On-Chip Cytometry using Plasmonic Nanoparticle Enhanced Lensfree Holography

Wei

McLeod

et al. 2013

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Computational microscopy tools, in particular lensfree on-chip imaging, provide a large field-of-view along with a long depth-of-field, which makes it feasible to rapidly analyze large volumes of specimen using a compact and light-weight on-chip imaging architecture. To bring molecular specificity to this high-throughput platform, here we demonstrate the use of plasmon-resonant metallic nanoparticles to automatically recognize different cell types based on their plasmon-enhanced lensfree holograms, detected and reconstructed over a large field-of-view of e.g., ~24 mm2.

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Optofluidic Tomography on a Chip

Cited by 71 publications

References 20 publications

Nano-imaging enabled via self-assembly

Nano-imaging enabled via self-assembly

Spectral Demultiplexing in Holographic and Fluorescent On-chip Microscopy

On-Chip Cytometry using Plasmonic Nanoparticle Enhanced Lensfree Holography

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