Despite the rapid progress in optical imaging, most of the advanced microscopy modalities still require complex and costly set-ups that unfortunately limit their use beyond well equipped laboratories. In the meantime, microscopy in resource-limited settings has requirements significantly different from those encountered in advanced laboratories, and such imaging devices should be costeffective, compact, light-weight and appropriately accurate and simple to be usable by minimally trained personnel. Furthermore, these portable microscopes should ideally be digitally integrated as part of a telemedicine network that connects various mobile health-care providers to a central laboratory or hospital. Toward this end, here we demonstrate a lensless on-chip microscope weighing 46 grams with dimensions smaller than 4.2cm × 4.2cm × 5.8cm that achieves sub-cellular resolution over a large field of view of ~24 mm 2 . This compact and light-weight microscope is based on digital in-line holography and does not need any lenses, bulky optical/mechanical components or coherent sources such as lasers. Instead, it utilizes a simple light-emitting-diode (LED) and a compact optoelectronic sensor-array to record lensless holograms of the objects, which then permits rapid digital reconstruction of regular transmission or differential interference contrast (DIC) images of the objects. Because this lensless incoherent holographic microscope has orders-of-magnitude improved light collection efficiency and is very robust to mechanical misalignments it may offer a cost-effective tool especially for telemedicine applications involving various global health problems in resource limited settings.
We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm2. By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.
We report a portable lensless on-chip microscope that can achieve <1 μm resolution over a wide field-of-view of ~24 mm2 without the use of any mechanical scanning. This compact on-chip microscope weighs ~95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.
We present a lens-free optical tomographic microscope, which enables imaging a large volume of approximately 15 mm 3 on a chip, with a spatial resolution of <1 μm× < 1 μm× < 3 μm in x, y and z dimensions, respectively. In this lens-free tomography modality, the sample is placed directly on a digital sensor array with, e.g., ≤4 mm distance to its active area. A partially coherent light source placed approximately 70 mm away from the sensor is employed to record lens-free in-line holograms of the sample from different viewing angles. At each illumination angle, multiple subpixel shifted holograms are also recorded, which are digitally processed using a pixel superresolution technique to create a single high-resolution hologram of each angular projection of the object. These superresolved holograms are digitally reconstructed for an angular range of AE50°, which are then back-projected to compute tomograms of the sample. In order to minimize the artifacts due to limited angular range of tilted illumination, a dual-axis tomography scheme is adopted, where the light source is rotated along two orthogonal axes. Tomographic imaging performance is quantified using microbeads of different dimensions, as well as by imaging wild-type Caenorhabditis elegans. Probing a large volume with a decent 3D spatial resolution, this lens-free optical tomography platform on a chip could provide a powerful tool for high-throughput imaging applications in, e.g., cell and developmental biology. L ight microscopy has been an irreplaceable tool in life sciences for several centuries. The quest to resolve smaller features with better resolution and contrast has improved the capabilities of this important tool at the cost of relatively increasing its size and complexity (1). On the other hand, we have experienced the flourishing of emerging technologies such as microfluidic and lab-on-a-chip systems, which offer fast and efficient handling and processing of biological samples within highly miniaturized architectures (2-7). The optical inspection of the specimen, however, is still being performed by conventional light microscopes, which has in general several orders of magnitude size mismatch compared to the scale of the microfluidic systems. As a result, there is a clear need for alternative compact microscopy modalities toward integration with miniaturized lab-on-a-chip platforms (8).The push for new optical microscopy modalities is not solely driven by the need for miniaturization and microfluidic integration. The fact that high resolution is achieved at the cost of significant field-of-view (FOV) reduction is another fundamental limitation of lens-based imaging. The relatively small FOV of conventional light microscopy brings additional challenges for its application to several important problems such as rare cell imaging or optical phenotyping of model organisms (9-15), where high-throughput microscopy is highly desired.In order to provide complementary solutions to these aforementioned needs, several lens-free digital microscopy techniqu...
We consider the tunneling current through a double point-contact Fabry-Pérot interferometer such as used in recent experimental studies of the fractional quantum Hall plateau at filling fraction =5/ 2. We compare the predictions of several different models of the state of the electrons at this plateau: the Moore-Read, antiPfaffian, SU͑2͒ 2 NAF, K = 8 strong pairing, and ͑3,3,1͒ states. All of these predict the existence of charge e / 4 quasiparticles, but the first three are non-Abelian while the last two are Abelian. We give explicit formulas for the scaling of charge e / 2 and charge e / 4 quasiparticle contributions to the current as a function of temperature, gate voltage, and distance between the two point contacts for all three models. Based on these, we analyze several possible explanations of two phenomena reported for recent experiments by Willett et al., namely, halving of the period of the observed resistance oscillations with rising temperature and alternation between the same two observed periods at low temperatures as the area of the interference loop is varied with a side gate. We conclude that the most likely explanation is that the observed alternation is due to switching between even and odd numbers of charge e / 4 quasiparticles enclosed within the loop as a function of side-gate voltage, which is a clear signature of the presence of non-Abelian anyons. However, there are important features of the data which do not have a simple explanation within this picture. We suggest further experiments which could help rule out some possible scenarios. We make the corresponding predictions for future tunneling and interference experiments at the other observed second Landau level fractional quantum Hall states.
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