2013
DOI: 10.1063/1.4813905
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Optofluidic manipulation of Escherichia coli in a microfluidic channel using an abruptly tapered optical fiber

Abstract: We report stable optical trapping and controlled manipulation of Escherichia coli cells in a microfluidic channel using an abruptly tapered optical fiber with 980-nm wavelength laser light launched. Stability of the trapping at different optical powers (10–70 mW) was demonstrated in fluids under different flow directions and velocities. The experimental results were supported by finite-element simulations and analytic calculations.

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Cited by 27 publications
(24 citation statements)
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“…With this probe the trapping of yeast cells was achieved. After this work several others reported similar OFTs fabrication procedures [30], [31]. The main difference among the fiber probes reported rely on the final optical fiber taper profiles and their focusing capabilities.…”
Section: B Thermal Pullingmentioning
confidence: 92%
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“…With this probe the trapping of yeast cells was achieved. After this work several others reported similar OFTs fabrication procedures [30], [31]. The main difference among the fiber probes reported rely on the final optical fiber taper profiles and their focusing capabilities.…”
Section: B Thermal Pullingmentioning
confidence: 92%
“…Exploring the effects of push, pulling and trapping at different distance of the focal point, the OFTs were used to perform trapping and arrangement (linear chains) of multiple particles [34]. Also, a sharper probe (380 nm diameter) was used in a microfluidic platform to study E-coli bacteria dynamics [30]. Rather than a standard taper a more abrupt profile yielding larger focusing distances and enabling truly contactless trapping of E-coli bacteria [31] was tested.…”
Section: B Thermal Pullingmentioning
confidence: 99%
“…To the best of our knowledge, there was no report on optical tweezers with tunable manipulation length. Very recently the combination of microfluidics with fiber tip tweezers, named optofluidic manipulation by Li and his group members, further enhanced the power and flexibility of optical manipulation methods by introducing another important factor, i.e., the microfluidic flow rate [23].…”
Section: Introductionmentioning
confidence: 99%
“…In comparison with the orientation of nanoscale rod-shaped objects using COTs, in which different orientations of the objects can be achieved using differently polarized laser beams, this is a disadvantage of our method for the orientation of MWCNTs. Although we have used tapered optical fiber to manipulate various objects, 30,32,33 the objects that can be thus trapped and manipulated are limited to microscale/submicroscale dielectric particles (the smallest are 0.7-mm SiO 2 particles) and microscale/submicroscale biological cells (the smallest are Escherichia coli cells, with a diameter of approximately 700 nm and a length of approximately 2.0 mm). These objects can be easily observed using an optical microscope, and their Brownian motion is not significant because of their large sizes.…”
Section: ð2þmentioning
confidence: 99%