Optimum Conditions for N-Acetyl Glucosamine Production From Tiger Shrimp (Penaeus Monodon) Shell by Serratia Marcescens

Halim, Yuniwaty; Hardoko, Hardoko; Handayani, Ratna; Lucida, Vania

doi:10.22159/ajpcr.2018.v11i12.28956

Cited by 4 publications

(9 citation statements)

References 15 publications

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“…The preparation of P, vannamei shrimp shells powder was performed according to the method by Halim et al (2018). The shells of P. vannamei were washed with clean water and dried under the sun for two days.…”

Section: Preparation Of P Vannamei Shrimp Shells Powdermentioning

confidence: 99%

“…Copyright ©2021 Faculty of Fisheries and Marine Universitas Airlangga November 2021 using UV-Vis spectrophotometer at wavelength of 324 nm, according to method by Halim et al (2018). Based on the highest N-acetylglucosamine concentration obtained from the fermentation, the optimum papaya trunk wood size and ratio (w/v) between papaya trunk wood and growth medium were determined.…”

Section: Immobilization Of P Stuartii Cells In Papaya Trunkmentioning

confidence: 99%

“…The images of sample were taken using two types of detectors, i.e., SE (secondary electron) and BSE (back scattered electron). (Halim et al, 2018). Furthermore, pH of medium was adjusted to 7.0 as it is the optimum pH for the growth of P. stuartii (Ayangbenro, 2017).…”

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

“…To determine the optimum size of papaya trunk wood and ratio between papaya trunk wood and growth medium, submerged fermentation using immobilized cells was carried out. Fermentation medium was prepared according to Halim et al (2018) with modification. The medium consisted of 10% of P. vannamei shrimp shells powder (w/v), 0.03% KH 2 PO 4 , 0.07% K 2 HPO 4 , 0.01% MgSO 4 .7H 2 O, and 0.7% (NH 4 ) 2 SO 4 as nitrogen source in 300 ml distilled water.…”

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

“…Afterwards, centrifugation (4000 rpm, 15 min) and filtration using Whatmann No. 1 filter paper were done (Halim et al, 2018). Filtrate containing N-acetylglucosamine was measured for its absorbance…”

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

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Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Halim

Tantradjaja

Hardoko

et al. 2021

JIPK

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Highlight Research AbstractChitin is a natural compound found abundantly in shrimp shells. Chitin can be degraded to produce N-acetylglucosamine, which has wide applications in the food and pharmaceutical fields. Fermentation using chitinolytic microorganisms can be used to produce N-acetylglucosamine from shrimp shells’ chitin. One of the strong chitinolytic bacteria that was isolated from previous research was Providencia stuartii. To provide better stability and efficiency in fermentation, P. stuartii cells were immobilized using entrapment method in papaya trunk wood. The aims of this research were to determine the optimum papaya trunk wood size, ratio of papaya trunk wood and growth medium, as well as the optimum fermentation cycle to produce N-acetylglucosamine from P. vannamei shrimp shells using submerged fermentation method. The research used experimental method with treatment of different sizes of papaya trunk wood (1 x 1 x 1 cm3, 1.5 x 1.5 x 1.5 cm3, and 2 x 2 x 2 cm3), different ratio of papaya trunk wood and growth medium (1:10, 1:15 and 1:20), and 4 fermentation cycles. Results showed that papaya trunk wood with size of 1 x 1 x 1 cm3 and ratio (w/v) of 1:10 could immobilize 87.08±2.05% of P. stuartii cells and produce the highest N-acetylglucosamine concentration, which was 238177.78±3153.48 ppm. The highest N-acetylglucosamine production was obtained from first fermentation cycle and decreased over the last three cycles, but still produced high concentration of N-acetylglucosamine. Therefore, it is possible to perform continuous N-acetylglucosamine production from shrimp shells using P. stuartii cells immobilized in papaya trunk wood.

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Section: Preparation Of P Vannamei Shrimp Shells Powdermentioning

confidence: 99%

Section: Immobilization Of P Stuartii Cells In Papaya Trunkmentioning

confidence: 99%

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Section: Microscopical Observation Using Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

See 3 more Smart Citations

Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Halim

Tantradjaja

Hardoko

et al. 2021

JIPK

Self Cite

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Microbial production of N-acetyl-D-glucosamine (GlcNAc) for versatile applications: Biotechnological strategies for green process development

Das,

Chowdhury,

Kumar

et al. 2024

Carbohydrate Research

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Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

Halim

Devianita

Hardoko

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. Shrimp shells contain chitin that can further be processed into N-acetylglucosamine which has been extensively used to treat joint damage. Providencia stuartii isolated form previous research has strong chitinolytic activity and may be utilized in the form of immobilized cells to be used in repeated fermentation. Pumice is a porous and rigid stone that offers superior mechanical strength, making it suitable to be used for immobilization process. Experimental approach. The research used experimental method to conduct the submerged fermentation process with different pumice stone size and pumice stone:growth medium ratio (m/V). The fermentation was carried out for 4 days at 37 C and pH of 7.0. The optimum pumice stone size and pumice stone:growth medium ratio (m/V) were used to determine the optimum fermentation cycle to produce N-acetylglucosamine. Results and conclusions. Pumice stones of 1.0×1.0×1.0 cm and pumice stone:growth medium ratio (m/V) of 1:5 were found to be the optimum conditions which successfully immobilized (89.99±1.65) % cells and produced (331.37±7.34) g/L N-acetylglucosamine. The highest N-acetylglucosamine concentration of (322.97±2.46) g/L was obtained in the first fermentation cycle which then decreased and remained stable throughout the last three cycles of fermentation. Novelty and scientific contribution. P. stuartii was a strong chitinolytic bacteria previously isolated from rotten shrimp shells and was used for the first time in immobilized form to produce N-acetylglucosamine. The findings in this research showed potential use of P. stuartii cells immobilized in pumice stone for continuous production of N-acetylglucosamine using fermentation method.

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Optimum Conditions for N-Acetyl Glucosamine Production From Tiger Shrimp (Penaeus Monodon) Shell by Serratia Marcescens

Cited by 4 publications

References 15 publications

Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Microbial production of N-acetyl-D-glucosamine (GlcNAc) for versatile applications: Biotechnological strategies for green process development

Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

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