Optimizing the Reduction of Molybdate by Two Novel Thermophilic Bacilli Isolated from Sinai, Egypt

Saeed, Ali; Sayed, Hayam A. E.; El-Shatoury, Einas H.

doi:10.1007/s00284-020-01874-y

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…It is known that thermophilic microorganisms have a diversity that includes both bacteria and archaea, and the most frequently isolated thermophilic strains have also been reported to belong to the Bacillus genus (Abootalebi et al 2020 ; Ladeira et al 2015 ; Shen et al 2021 ; Verma et al 2018 ; Wang et al 2006 ). The presence of thermophilic Bacilli has been investigated in hot springs and compost sources around the world (Yanmis et al 2015 ), such as those in India (Verma et al 2018 ), Iran (Abootalebi et al 2020 ), Indonesia (Safitri et al 2020 ), Jordan (Mohammad et al 2017 ), Faisalabad (Saleh et al 2020 ), Tunisia (Salem et al 2020 ), Brazil (Bernardo et al 2020 ), Egypt (Saeed et al 2020 ), Malaysia (Msarah et al 2020 ), Vietnam (Dang et al 2018 ), Saudi Arabia (Al-Johani et al 2016 ), South Africa (Tsotetsi et al 2020 ) and Japan (Akita et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Uluçay

Görmez

Öziç

2022

Antonie van Leeuwenhoek

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In the last two decades, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring sources to explore the benefits of thermophiles in industrial and biotechnological fields. Within the scope of this study, a total of 83 thermophilic Bacilli strains were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 °C) located in the Eastern and Southeastern Anatolia Regions of Turkey. The physiological, morphological, biochemical and molecular properties of the isolates were determined. As a result of the 16S rRNA gene sequence analysis, 5 different species ( Bacillus licheniformis , Bacillus sp . , Bacillus subtilis , Geobacillus kaustophilus, and Weizmannia coagulans ,) were identified. B. licheniformis and B. subtilis were the most frequently encountered species among those obtained from the researched hot spring sources. Phylogenetic analysis was conducted to evaluate the phylogenetic relationships of the isolated species. The results showed that there was no significant difference between the groups and the bacteria in terms of the locations or optimum temperatures of the isolates. The bacterial isolates were screened for amylase, cellulase, lipase and protease hydrolytic enzyme activities. The hydrolytic enzyme production potentials among the isolates were identified in 68 (82%) isolates for amylase, 34 (41%) for cellulase, 69 (83%) for lipase and 73 (88%) for protease. All isolates were found to have at least one or more extracellular enzyme activities. Additionally, it was determined that 27 of the existing isolates (32.8%) were able to produce all of the aforementioned hydrolytic enzymes. Supplementary Information The online version contains supplementary material available at 10.1007/s10482-021-01678-5.

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Section: Introductionmentioning

confidence: 99%

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Uluçay

Görmez

Öziç

2022

Antonie van Leeuwenhoek

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“…In this study, a thermophilic isolate was recovered from the hot springs region, Pharaoh's Bath, Sinai, Egypt. Previous work by Saeed et al [23] reported the isolation of thermophilic bacteria from the same site. Mohammad et al [24] illustrated the isolation of closely related thermophiles from hot springs and deep sea in Jordon.…”

Section: Discussionmentioning

confidence: 94%

Statistical factorial designs for optimum production of thermostable α-amylase by the degradative bacterium Parageobacillus thermoglucosidasius Pharon1 isolated from Sinai, Egypt

Saeed

El-Shatoury

Sayed

2021

Journal of Genetic Engineering and Biotechnology

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Background This study aimed to isolate potent thermophilic and amylolytic bacteria from a hot spring of Pharaoh’s bath, Sinai, Egypt, and screen its degradative activity. The amylolytic activity was further optimized using a statistical full factorial design followed by response surface methodology. Results A thermophilic bacterium was isolated from the hot spring of Pharaoh’s Bath, Sinai, Egypt. The isolate produced amylase, cellulase, and caseinase and was identified by 16S rRNA gene sequencing as Parageobacillus thermoglucosidasius Pharon1 (MG965879). A growth medium containing 1% soluble starch was found to optimize the amylase production. Dinitrosalycalic acid method (DNS) was used to estimate the amount of reducing sugar produced. Statistical full factorial and response surface designs were employed to optimize physical variables affecting the α-amylase production and determine the significant interactions of the studied variables during the fermentation process. According to the results obtained by the response optimizer, the maximum amylase activity reached 76.07 U/mL/ min at 54.1°C, pH 5.6 after 98.5 h incubation under aerobic conditions. Moreover, the produced enzyme was thermostable and retained most of its activity when exposed to a high temperature of 100°C for 120 min. Maximum enzyme activity was attained when the enzyme was incubated at 70°C for 30 min. Conclusions This is the first report of the production of thermostable α-amylase by the potent thermophilic Parageobacillus thermoglucosidasius. The enzyme endured extreme conditions of temperature and pH which are important criteria for commercial and industrial applications.

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“…Statistical optimization approaches like response surface methodology (RSM) are commonly used to investigate responses after simultaneous variation of multiple process parameters (Tortella et al 2019 ; Zaki et al 2019 ; Saeed et al 2020 ; Zaki and El-Sayed 2021 ). By employing the response surface methodology, the nanocomposite fabrication showed a better response to variations in the different investigated parameters as pH, reaction time, AgNO 3 concentration and RH concentration.…”

Section: Discussionmentioning

confidence: 99%

Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

2022

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The bacterial wilt is a global destructive plant disease that initiated by the phytopathogenic Ralstonia solanacearum. This study display a novel biofabrication of silica/silver nanocomposite using Fusarium oxysporum-fermented rice husk (RH) under solid state fermentation (SSF). The biofabricated nanocomposite was characterized by XRD, UV–Vis. spectroscopy, DLS, SEM, EDX elemental mapping, and TEM analyses as well as investigated for anti-R. solanacearum activity. Response surface methodology was also processed for optimizing the biofabrication process and improving the anti-bacterial activity of the fabricated nanocomposite. Maximum suppression zone of 29.5 mm against R. solanacearum was reached at optimum RH content of 6.0 g, AgNO3 concentration of 2.50 mM, reaction pH of 6.3, and reaction time of 2 days. The anti-R. solanacearum activity of the fabricated nanocomposite was further improved by exposing the F. oxysporum strain to a gamma irradiation dose of 200 Gy. In conclusion, RH recycling under SSF by F. oxysporum could provide an innovative, facile, non-expensive, and green approach for fabricating SiO2/Ag nanocomposite that could be applied efficiently as an eco-friendly antibacterial agent to combat R. solanacearum in agricultural applications. Moreover, the developed method could serve as a significant platform for the designing of new nanostructures for broad applications.

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Optimizing the Reduction of Molybdate by Two Novel Thermophilic Bacilli Isolated from Sinai, Egypt

Cited by 14 publications

References 26 publications

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey

Statistical factorial designs for optimum production of thermostable α-amylase by the degradative bacterium Parageobacillus thermoglucosidasius Pharon1 isolated from Sinai, Egypt

Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

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