Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

Zaki, Amira G; Hasanien, Yasmeen A; El-Sayyad, Gharieb S.

doi:10.1186/s13568-022-01372-3

Cited by 11 publications

(3 citation statements)

References 72 publications

(96 reference statements)

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“…This prevented an impact on other bacteria from the environment, which is necessary for a safe biocontrol agent (Buttimer et al, 2017). The bacteriophage specificity to different strains of R. solanacearum was investigated in studies from Japan and Spain (Biosca et al, 2019;Zaki et al, 2022b). This specificity might be explained by the fact that the host range of bacteriophages is restricted to particular bacterial populations with whom they have co-evolved.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophages as Promising Agents for Biocontrol of Ralstonia solanacearum Causing Bacterial Wilt Disease

Hasanien,

Abdel-Aal,

Younis

et al. 2023

Egypt. J. Bot.

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T O COMBAT the growing antibiotic and chemical resistance in bacterial phytopathogens like Ralstonia solanacearum, which causes bacterial wilt disease, bacteriophages are an efficient biocontrol technique and an environmentally benign therapy.This study reports the isolation and characterization of three phages, designated ɸRS1, ɸRS2, and ɸRS3, for their effectiveness against R. solanacearum EMCC 1274 in a bioassay using Solanum lycopersicum (tomato) pots. It was discovered that the three isolated bacteriophages were viable and stable over a wide pH range (3.0-10.0) and at temperatures as high as 80°C. The phage ɸRS1 was measured using a transmission electron microscope to be approximately 291.84nm in size, with an icosahedral head (134.4nm) and tail, while also belonging to the Myoviridae family. The phage ɸRS2 is almost 232nm in size, with an icosahedral head (116nm) and a tail, and it belongs to the Myoviridae family, while the phage ɸRS3 is roughly 88.32 nm in size, with an icosahedral head (69.12nm) and a short, non-contractile tail, and it belongs to the Podoviridae family. In comparison to just pathogen-treated controls, plant bioassays demonstrated 91.3, 95.6 and 95.6% reversal of disease symptoms employing phages ɸRS1, ɸRS2, and ɸRS3 respectively. A biocontrol formulation for preventing the bacterial wilt disease caused by R. solanacearum appears to be developable using a combination of isolated bacteriophages (100% reversal in disease symptoms).

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Section: Discussionmentioning

confidence: 99%

Bacteriophages as Promising Agents for Biocontrol of Ralstonia solanacearum Causing Bacterial Wilt Disease

Hasanien,

Abdel-Aal,

Younis

et al. 2023

Egypt. J. Bot.

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“…Response surface methodology, including central composite and Box–Behnken, is a statistical modelling tool for estimating the behaviour of process parameters and understanding their interactions on the investigated response (process output) (El‐Sayed & Zaki, 2022; Saeed et al, 2020, 2021; Zaki et al, 2019, 2021, 2022; Zaki & El‐Sayed, 2021) Thus, RSM is an efficient methodology for improving and optimizing bioprocesses. In literature, Ahmady‐Asbchin (2016) proved the efficiency of the Box–Behnken model of RSM using Design‐Expert software to optimize Cd +2 biosorption on Pseudomonas aeruginosa with a maximum capacity of 0.56 mmol g −1 .…”

Section: Discussionmentioning

confidence: 99%

Biosorption optimization of lead(II) and cadmium(II) ions by two novel nanosilica-immobilized fungal mutants

Zaki

Hasanien

Abdel‐Razek

2022

Journal of Applied Microbiology

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Aims:This study aims at immobilization of fungal mutants on nanosilica (NSi)carriers for designing efficient biosorbents as a significant new technology for decontamination practices and maximizing their heavy metal (HM) sorption proficiency through the experimental design methodology. Materials and Results: Endophytic fungal mutant strains, Chaetomium globosumEl26 mutant and Alternaria alternata S5 mutant were heat inactivated and then immobilized, each separately, on NSi carriers to formulate two separated nanobiosorbents. The formulated NSi-Chaetomium globosum El26 mutant (NSi-Chae El26 m) was investigated for Pb +2 uptake while, the formulated NSi-Alternaria alternata S5 mutant (NSi-Alt S5 m) was investigated for Cd +2 uptake, each through a batch equilibrium protocol. Before and after the metal sorption process, the designed nano-biosorbents were characterized via scanning electron microscopy, energydispersive X-ray spectroscopy, and Fourier transform infrared analysis. Sorption pH, contact time, sorbent concentration, and initial HM concentration were statistically optimized using a Box-Behnken design. Results showed that NSi-Chae El26 m was efficient in Pb +2 uptake with maximum biosorption capacities of 199.0, while NSi-Alt S5 m was efficient in Cd +2 uptake with maximum biosorption capacities of 162.0 mg g −1 . Moreover, the equilibrium data indicated that the adsorption of Pb +2 and Cd +2 by the tested nano-biosorbents fitted to the Freundlich isotherm. Conclusions: The formulated nano-biosorbents resulted in higher HM biosorption of metal ions from aqueous solution than that obtained by the free fungal biomass.The biosorption statistical modelling described the interactions between the tested sorption parameters and predicted the optimum values for maximum HM biosorption capacity by the two designed nano-biosorbents.Significance and Impact of The Study: These findings verify that members of the endophytic fungal genera Alternaria and Chaetomium are suitable to produce nanobiosorbents for decontamination practices after treatment by gamma mutagenesis, heat inactivation, and NSi immobilization. Moreover, statistical optimization can assist to evaluate the optimal conditions to produce such bioremediation material.

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“…The diffracted X-rays' intensity was calculated as diffracted angle 2θ (El-Batal et al 2020). The most prevailing Se NPs particle size distribution, hydrodynamic radius, and polydispersity index (PDI) were determined by Dynamic Light Scattering (Zaki et al 2022) (DLS-PSS-NICOMP 380-USA). Further, the mean particle size, the microstructure, and the estimated shape of the biosynthesized Se NPs were assessed by High-Resolution Transmission Electron Microscope (HRTEM, JEM2100, Jeol, Japan) (Ashour et al 2018).…”

Section: Characterization Of the Synthesized Biogenic Se Npsmentioning

confidence: 99%

Selenium nanoparticles induce growth and physiological tolerance of wastewater‑stressed carrot plants

et al. 2023

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Climate changes have a direct impact on agricultural lands through their impact on the rate of water levels in the oceans and seas, which leads to a decrease in the amount of water used in agriculture, and therefore the use of alternative sources of irrigation such as wastewater and overcoming its harmful effect on plants was one of the solutions to face this problem. In the present study, the impacts of the synthesized selenium nanoparticles (Se NPs) alone or in combination with glycine betaine and proline treatments on the growth, physiological, and yield attributes of wastewater irrigated carrot plants are investigated. Furthermore, to evaluate heavy metals uptake and accumulation in edible plant parts. The usage of wastewater to carrot plants significantly increased free proline contents, total phenols, superoxide dismutase, catalase, peroxidase, polyphenol oxidase, Malondialdehyde (MDA), and hydrogen peroxide (H2O2) throughout the two growth stages. While total soluble carbohydrate and soluble protein content in carrot shoots and roots were significantly reduced. Moreover, the concentrations of nickel (Ni), cadmium (Cd), lead (Pb), and cobalt (Co) in carrot plants were considerably higher than the recommended limits set by international organizations. Application of selenium nanoparticles alone or in combination with glycine betaine and proline reduced the contents of Ni, Cd, Pb, and Co; free proline; total phenols; superoxide dismutase; catalase; peroxidase; polyphenol oxidase; Malondialdehyde (MDA) and Hydrogen peroxide (H2O2) in carrot plants. However, morphological aspects, photosynthetic pigments, soluble carbohydrates, soluble protein, total phenol, and β-Carotene were enhanced in response to Se NPs application. As an outcome, this research revealed that Se NPs combined with glycine betaine and proline can be used as a strategy to minimize heavy metal stress caused by wastewater irrigation in carrot plants, consequently enhancing crop productivity and growth.

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Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

Cited by 11 publications

References 72 publications

Bacteriophages as Promising Agents for Biocontrol of Ralstonia solanacearum Causing Bacterial Wilt Disease

Bacteriophages as Promising Agents for Biocontrol of Ralstonia solanacearum Causing Bacterial Wilt Disease

Biosorption optimization of lead(II) and cadmium(II) ions by two novel nanosilica-immobilized fungal mutants

Selenium nanoparticles induce growth and physiological tolerance of wastewater‑stressed carrot plants

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