Optimizing gold nanoparticle seeding density on DNA origami

Gates, Elisabeth P; Jensen, J. K.; Harb, John N.; Woolley, Adam T.

doi:10.1039/c4ra15451g

Cited by 18 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to maximize LSPR, a interparticle gap of ∼2 nm was chosen in the DNA origami design. With standard functionalization procedures, it is challenging to achieve precise alignment and high yield at the same time. ,− For instance, when using closely spaced binding sites, single AuNPs often bind to two neighboring binding positions simultaneously. Furthermore, a large excess of AuNPs has to be used to prevent interstructure cross-linking, followed by laborious purification steps.…”

Section: Resultsmentioning

confidence: 99%

Self-Assembled Active Plasmonic Waveguide with a Peptide-Based Thermomechanical Switch

et al. 2016

View full text Add to dashboard Cite

Nanoscale plasmonic waveguides composed of metallic nanoparticles are capable of guiding electromagnetic energy below the optical diffraction limit. Signal feed-in and readout typically require the utilization of electronic effects or near-field optical techniques, whereas for their fabrication mainly lithographic methods are employed. Here we developed a switchable plasmonic waveguide assembled from gold nanoparticles (AuNPs) on a DNA origami structure that facilitates a simple spectroscopic excitation and readout. The waveguide is specifically excited at one end by a fluorescent dye, and energy transfer is detected at the other end via the fluorescence of a second dye. The transfer distance is beyond the multicolor FRET range and below the Abbé limit. The transmittance of the waveguide can also be reversibly switched by changing the position of a AuNP within the waveguide, which is tethered to the origami platform by a thermoresponsive peptide. High-yield fabrication of the plasmonic waveguides in bulk was achieved using silica particles as solid supports. Our findings enable bulk solution applications for plasmonic waveguides as light-focusing and light-polarizing elements below the diffraction limit.

show abstract

Section: Resultsmentioning

confidence: 99%

Self-Assembled Active Plasmonic Waveguide with a Peptide-Based Thermomechanical Switch

et al. 2016

View full text Add to dashboard Cite

show abstract

“…If nanotubes were used as channels or tracks for transportation of molecular cargo, dynamic rearrangement of nanotube connections between termini anchored at different points on a surface or on different objects could be used to regulate when and where signals or materials are transported. Nanotubes could also serve as templates for other materials such as electrically conductive nanoparticles, and self-assembling electrical circuits that could dynamically rewire could be developed. These examples illustrate how selective, molecular scale regulation of nanostructures that direct nanotube network connectivity could induce structural changes over length scales of 10 μm or more, 1–2 orders of magnitude larger than are induced using most reconfigurable DNA nanostructures. − …”

Section: Discussionmentioning

confidence: 99%

Reconfiguring DNA Nanotube Architectures via Selective Regulation of Terminating Structures

et al. 2020

View full text Add to dashboard Cite

Molecular assemblies inside cells often undergo structural reconfiguration in response to stimuli to alter their function. Adaptive reconfiguration of cytoskeletal networks, for example, enables cellular shape change, movement, and cargo transport and plays a key role in driving complex processes such as division and differentiation. The cellular cytoskeleton is a self-assembling polymer network composed of simple filaments, so reconfiguration often occurs through the rearrangement of its component filaments' connectivities. DNA nanotubes have emerged as promising building blocks for constructing programmable synthetic analogs of cytoskeletal networks. Nucleating seeds can control when and where nanotubes grow, and capping structures can bind nanotube ends to stop growth. Such seeding and capping structures, collectively called termini, can organize nanotubes into larger architectures. However, these structures cannot be selectively activated or inactivated in response to specific stimuli to rearrange nanotube architectures, a key property of cytoskeletal networks. Here, we demonstrate how selective regulation of the binding affinity of DNA nanotube termini for DNA nanotube monomers or nanotube ends can direct the reconfiguration of nanotube architectures. Using DNA hybridization and strand displacement reactions that specifically activate or inactivate four orthogonal nanotube termini, we demonstrate that nanotube architectures can be reconfigured by selective addition or removal of distinct termini. Finally, we show how terminus activation could be a sensitive detector and amplifier of a DNA sequence signal. These results could enable the development of adaptive and multifunctional materials or diagnostic tools.

show abstract

“…A common strategy for site-specific attachment of Au seeds to a DNA origami template is to use thiol-modified DNA to form Au NP-DNA conjugates [ 25 ]. Gates et al [ 65 ] investigated parameters such as time of hybridization, ratio of seeds to templates, and concentration of Mg 2+ to optimize the seeding density and yield. They found that 30–90 min hybridization times, 1.8–4.5 ratio of Au NPs per attachment location, and 70–100 mM Mg 2+ were best suited for making thinner Au NWs that appeared continuous in SEM imaging.…”

Section: Metallization Of Dna Templatesmentioning

confidence: 99%

Bottom-Up Fabrication of DNA-Templated Electronic Nanomaterials and Their Characterization

et al. 2021

View full text Add to dashboard Cite

Bottom-up fabrication using DNA is a promising approach for the creation of nanoarchitectures. Accordingly, nanomaterials with specific electronic, photonic, or other functions are precisely and programmably positioned on DNA nanostructures from a disordered collection of smaller parts. These self-assembled structures offer significant potential in many domains such as sensing, drug delivery, and electronic device manufacturing. This review describes recent progress in organizing nanoscale morphologies of metals, semiconductors, and carbon nanotubes using DNA templates. We describe common substrates, DNA templates, seeding, plating, nanomaterial placement, and methods for structural and electrical characterization. Finally, our outlook for DNA-enabled bottom-up nanofabrication of materials is presented.

show abstract

Optimizing gold nanoparticle seeding density on DNA origami

Cited by 18 publications

References 35 publications

Self-Assembled Active Plasmonic Waveguide with a Peptide-Based Thermomechanical Switch

Self-Assembled Active Plasmonic Waveguide with a Peptide-Based Thermomechanical Switch

Reconfiguring DNA Nanotube Architectures via Selective Regulation of Terminating Structures

Bottom-Up Fabrication of DNA-Templated Electronic Nanomaterials and Their Characterization

Contact Info

Product

Resources

About