Optimized production of lipase from Bacillus subtilis PCSIRNL-39

Mazhar, Haniya; Abbas, Naaz; Ali, Sakhawat; Sohail, Amir; Hussain, Zahid; Ali, Syed Shahid

doi:10.5897/ajb2017.15924

Cited by 33 publications

(7 citation statements)

References 19 publications

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“…and P. aeruginosa was recorded at 73.50 ± 0.44 and 37.05 ± 0.75 U/ml, respectively, which were found to be 3.18 and 6.74-fold higher than before. Similarly, the maximum output of lipase was reported by Mazhar et al (2017) for the Bacillus subtilis PCSIRNL-39 strain at 5% inoculum concentration. For maximum lipase production, pH 7, glucose 2.0% and peptone 1.5% were used as production medium with 5% fresh inoculum.…”

Section: Resultssupporting

confidence: 55%

Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments

Aktar,

Saha

2023

Bangladesh J. Bot.

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The present study aims to identify prospective lipase-producing bacteria and optimize different culture parameters to produce maximum lipase. A total of 37 lipase-positive bacteria were isolated from lipid-rich environments. Among them, strains S1N-2 and S10P-1 were found to have potential and were molecularly identified as Stenotrophomonas species ICMM10 and Pseudomonas aeruginosa BA7823, respectively. For maximum lipase production, the optimum pH was 7 and the temperature was 37ºC. Using these conditions, the isolates were grown on media with additional carbon and nitrogen sources along with different inoculum concentrations. The maximum amount of lipase produced by Stenotrophomonas sp. ICMM10 was 136.47 ± 1.63 U/ml, which is 5-fold higher than before. Pseudomonas aeruginosa BA7823 produced a maximum lipase of 96.33 ± 0.21 U/ml, which was about 9-fold higher than before. These results would provide useful information about lipase production by indigenous isolates, which may be employed as a potential bacterial lipase source of biotechnological interest. Bangladesh J. Bot. 52(4): 979-988, 2023 (December)

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Section: Resultssupporting

confidence: 55%

Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments

Aktar,

Saha

2023

Bangladesh J. Bot.

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“…reported by Sharma et al (2001) can grow at higher temperature like 50°C. B. subtilis strain IFFI 10210 reported by Ma et al (2006) and B. subtilis PCSIR NL-39 showed 45ºC as the optimum temperature for bacterial growth and enzyme production (Mazhar et al, 2016;2017).…”

Section: Discussionmentioning

confidence: 99%

Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Mazhar¹,

Abbas²,

Zamir³

et al. 2018

Punjab Univ. J. Zool.

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In this study, the optimum growth conditions and lipolytic enzyme activity shown by the bacterial isolate (PCSIR NL-37) from the soil of Lahore (Punjab, Pakistan) are being reported. Lipolytic bacterial strains (100) isolated from soil and water samples from oil industry, mobile oil filling areas and kitchen wastages were screened on tributyrin agar and rhodamine agar plates. The strain PCSIR NL-37 was identified as Bacillus cereus following morphological, biochemical and molecular characterization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nitrogen source, showing 55U/mL enzyme activity. The best inoculum size for the growth of this strain was 4-5%. Optimum pH was 8 and best temperature was 40 o C for the reported strain. Addition of divalent Mg 2+ and Ca 2+ ions resulted in 2-fold increase in enzyme activity while SDS completely inhibit the lipase activity to zero.

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“…Interactions among the three factors are illustrated (Figure 6). Many authors applied Plackett–Burman experimental design for the enhancement of enzyme production (Collaa et al, 2016; Kai and Peisheng, 2016; Vasiee et al, 2016; Ebrahimipour et al, 2017; Mazhar et al, 2017). …”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications.

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Optimized production of lipase from Bacillus subtilis PCSIRNL-39

Cited by 33 publications

References 19 publications

Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments

Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments

Optimization Study of Lipolytic Enzyme from Bacillus cereus, PCSIR NL-37

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

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