Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Hassan, Sahar W.M.; Latif, Hala H. Abd El; Ali, Safaa M.

doi:10.3389/fmicb.2018.02377

Cited by 59 publications

(24 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Corn oil has been used as a substrate to produce lipases by Bacillus cereus HSS [19]. The presence of lipase is indicated by an orange glow when viewed under UV light at a wavelength of 365 nm (table 1).…”

Section: Resultsmentioning

confidence: 99%

Optimization of Lactobacillus Plantarum Activities in the Biosinthesis of Lipase Enzymes

Puspadewi¹,

Adirestuti²,

Dewi³

et al. 2021

Int J App Pharm

View full text Add to dashboard Cite

Objective: Lipase was protein compounds that can be used for many human activities. Its main function was to degrade fat including 'wrapping' cholesterol which make easily flowed in the blood. The presence lipase was important because can help the digestive healthy. These enzyme can catalyze a variety of reactions including hydrolysis, alcoholysis, esterification and aminolysis. Lipase was utilized in various sectors, such as fat, oil, milk and pharmaceutical industries. This enzyme biosynthesis can be carried out by Pseudomonas aeruginosa, Lactobacillus plantarum and Aspergillus niger. Methods: The process through fermentation techniques in lipid containing substrates under optimal conditions required by microorganisms. The fermentation products produced were tested for the presence of lipase enzymes qualitatively and quantitatively. The biosynthesis process can be influence by changes in pH, temperature and the presence of glucose. This study aimed to determine the ability of L. plantarum to produce lipases with vegetables oil substrates. The research used L. plantarum carried out at 37 °C for 24-48 h and pH 6-8 in the vegetable oil substrates. Results: The fermentation products showed hydrolysis reaction to the test media containing oil lipid with lipase levels of 2.708-3.3000 U/ml Conclusion: The results showed that Lactobacillus plantarum can synthesize the lipase enzyme in palm oil and corn oil as substrates.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization of Lactobacillus Plantarum Activities in the Biosinthesis of Lipase Enzymes

Puspadewi¹,

Adirestuti²,

Dewi³

et al. 2021

Int J App Pharm

View full text Add to dashboard Cite

show abstract

“…The results revealed that the standard protocol produced the highest concentration of DNA. Hassan et al [30,31] have also proven that the use of standard protocols produces higher concentrations than the DNA isolation kit for water (Epicenter).…”

Section: Molecular Methodsmentioning

confidence: 99%

“…Bacillus spp. bacteria such as B. alcalophilus, B. licheniformis, B. pumilus, and B. subtilis are the most well-known producers of bacterial lipases at present [30,31,68].…”

Section: Lipasesmentioning

confidence: 99%

Metagenomic applications in exploration and development of novel enzymes from nature: a review

Prayogo

Budiharjo

Kusumaningrum

et al. 2020

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background: Microbial community has an essential role in various fields, especially the industrial sector. Microbes produce metabolites in the form of enzymes, which are one of the essential compounds for industrial processes. Unfortunately, there are still numerous microbes that cannot be identified and cultivated because of the limitations of the culture-based method. The metagenomic approach is a solution for researchers to overcome these problems. The main body of the abstract: Metagenomics is a strategy used to analyze the genomes of microbial communities in the environment directly. Metagenomics application used to explore novel enzymes is essential because it allows researchers to obtain data on microbial diversity, reaching of 99% and various types of genes encoding an enzyme that has not yet been identified. Basic methods in metagenomics have been developed and are commonly used in various studies. A basic understanding of metagenomics for researchers is needed, especially young researchers to support the success of the research. Short conclusion: Therefore, this review was done in order to provide a deep understanding of metagenomics. It also discussed the application and basic methods of metagenomics in the exploration of novel enzymes, especially in the latest research. Several types of enzymes, such as cellulases, proteases, and lipases, which have been explored using metagenomics, were reviewed in this article.

show abstract

“…Future studies on this topic could reveal the features and properties of these "true" lipases from green macroalgae-associated bacteria. Lipases from marine sources have shown attractive characteristics such as thermostability [98], high-salt tolerance [99], cold-adaptation [100], extreme pH tolerance [101], organic solvents tolerance [102], and enantioselectivity [103]. These important properties are largely required in industrial and biotechnological applications such as plastic degradation [104] and biodiesel synthesis [105], as well as anti-biofilm and biofouling additives [106].…”

Section: Identification Of a "True" Lipasementioning

confidence: 99%

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

2020

View full text Add to dashboard Cite

Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total bacterial DNA isolation from the surface of U. lactuca thalli. Then, we designed sets of primers and used them directly for PCR amplification. The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. We obtained high molecular weight and good quality total bacterial DNA that served as a template to identify a fragment corresponding to an Acetyl-CoA C-Acetyltransferase (or Thiolase), and a candidate fragment for a versatile “true” lipase. We expressed the possible “true” lipase gene fragment heterologously in Escherichia coli and obtained proof of hydrolytic activity on Tributyrin, Tween-20, and Olive-oil media. This study resulted in new knowledge on U. lactuca-associated epiphytic bacteria as possible brand-new sources of enzymes such as thiolases and “true” lipases. However, future studies are required to describe the characteristics and important applications of these candidate enzymes.

show abstract

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Cited by 59 publications

References 58 publications

Optimization of Lactobacillus Plantarum Activities in the Biosinthesis of Lipase Enzymes

Optimization of Lactobacillus Plantarum Activities in the Biosinthesis of Lipase Enzymes

Metagenomic applications in exploration and development of novel enzymes from nature: a review

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

Contact Info

Product

Resources

About