Optimized Anti-pathogenic Agents Based on Core/Shell Nanostructures and 2-((4-Ethylphenoxy)ethyl)-N-(substituted-phenylcarbamothioyl)-benzamides

Abstract

The purpose of this study was to design a new nanosystem for catheter surface functionalization with an improved resistance to Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853 colonization and subsequent biofilm development. New 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides were synthesized and used for coating a core/shell nanostructure. Their chemical structures were elucidated by NMR, IR and elemental analysis, being in agreement with the proposed ones. Fe3O4/C12 of up to 5 nm size had been synthesized with lauric acid as a coating agent and characterized by XRD, FT-IR, TGA, TEM and biological assays. The catheter pieces were coated with the fabricated nanofluid in magnetic field. The microbial adherence ability was investigated in 6 multiwell plates by using culture based methods and Scanning Electron Microscopy (SEM). The nanoparticles coated with the obtained compounds 1a–c inhibited the adherence and biofilm development ability of the S. aureus and P. aeruginosa tested strains on the catheter functionalized surface, as shown by the reduction of viable cell counts and SEM examination of the biofilm architecture. Using the novel core/shell/adsorption-shell to inhibit the microbial adherence could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with improved anti-biofilm properties.

Hybrid nanostructured coating for increased resistance of prosthetic devices to staphylococcal colonization

“…We have suggested before the efficiency of nanosystem-vectored essential oil strategy [23]. The Fe 3 O 4 /C 12 nanoparticles seem not to be cytotoxic on the HEp2 cell line, which is a great advantage for the in vivo use of these nanostructure systems for biomedical applications with minor risks of the occurrence of side effects [48]. …”

Magnetite Nanostructures

“…The purpose of this study was to design a new nanosystem for catheter surface functionalization with improved resistance to S. aureus and P. aeruginosa colonization and subsequent biofilm development. Viable cell counts and SEM examination demonstrated that the nano-coated surfaces inhibited both the initial attachment and biofilm development of S. aureus and P. aeruginosa on the functionalized catheter surfaces (Limban et al, 2012).…”

“…The analysis showed that the tested nanobiosystem exhibited an intensive antibiofilm effect, as demonstrated by a low number of yeast cells that adhered to the coated surface (Anghel et al, 2012b). Limban et al (2012) reported the synthesis of new 2-((4-ethylphenoxy)methyl)-N-(substituted-phenylcarbamothioyl)-benzamides and their usage as coatings for a core/shell nanostructure. The purpose of this study was to design a new nanosystem for catheter surface functionalization with improved resistance to S. aureus and P. aeruginosa colonization and subsequent biofilm development.…”

Silver nanoparticles embedded into silica functionalized with vitamins as biological active materials

“…The assessment of the complexes influence on the microbial ability to colonize an inert substratum was performed by the microtiter method, following previously described protocols [34][35][36][37] …”