Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design

Wibisono, Kunto Aji; Aisyah, Syarifah Iis; Suhesti, Sri; Nurcholis, Waras

doi:10.20884/1.jm.2019.14.2.497

Cited by 10 publications

(7 citation statements)

References 27 publications

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“…The final unit of analysis was expressed as mol TE (Trolox Equivalent)/g extract. Wibisono et al (2019) modified. A total of 10 μL of sample solution was added with 50 μL of 0.1 M phosphate buffer with a pH of 7, 25 μL α-glucosidase 0.04 U mL -1 and 25 μL of p-nitrophenyl-α-D glucopyranoside 0.5 mM were inserted into a microplate.…”

Section: Antioxidant Frap Methodsmentioning

confidence: 99%

Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes

et al. 2021

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Abstract. Sahid ZD, Syukur M, Maharijaya A, Nurcholis W. 2021. Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes. Biodiversitas 22: 3838-3843. Chili is a horticultural crop with potential as a functional food crop. This is because chili has beneficial biochemical parameters such as antioxidants, phenolics, flavonoids, and ?-glucosidase inhibitors. This study evaluated the biochemical parameters in twelve genotypes of two chili species (Capsicum frutescens and Capsicum chinense). Information from this research can be used as a reference in chili plant breeding activities which are directed as functional food plants. The method of measuring biochemical parameters using an ELISA reader with a modified method. Samples were measured with a microplate and repeated three times. The results that emerged from the ELISA reader were then converted with Microsoft Excel and analyzed using the R program. The results showed that the Capsicum chinense species (Habanero Fransisca) had the highest ?-glucosidase inhibitory activity (52.52%) compared to other genotypes of the Capsicum frutescens species. The highest TPC (19.42), TFC (2.51), and antioxidant FRAP (117.27) were shown by the pure line genotype F8 285290-290-2-2-4-4-1. The biochemical content of the chili genotypes tested was divided into three major groups. This grouping is not affected based on the species used. The correlation between antioxidants using the FRAP method with TFC (0.69) and TPC (0.83) is positive and significantly different. In conclusion, information on biochemical content can be used as a basis for developing genotypes, especially for the purposes of plant breeding activities in the future.

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Section: Antioxidant Frap Methodsmentioning

confidence: 99%

Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes

et al. 2021

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“…Indonesia memiliki banyak potensi bahan alam obat herbal. Salah satu herbal yang sering digunakan ibu setelah melahirkan adalah daun Plectranthus amboinicus (Wibisono et al, 2019). Wanita Batak di Sumatera Utara, biasanya mengkonsumsi tanaman ini segera setelah melahirkan.…”

Section: Pendahuluanunclassified

“…Beberapa molekul fitoestrogen disebutkan, yaitu, diosgenin, estragole, silybin, shatavarine, kaempferol, dan quercetin, dapat menginduksi ekspresi reseptor prolaktin (PRLR), meningkatkan produksi kasein, dan aktivitas laktosa sintetase di sel epitel payudara (Tabares et al, 2014). Selain itu, senyawa flavonoid pada P. amboinicus (Wibisono et al, 2019) dapat merangsang pelepasan hormon prolaktin (PRL) dan hormon pertumbuhan (GH), up-regulated prolactin hormone receptor (PRLR), dan ekspresi gen GHR, serta merangsang perkembangan payudara pada tikus (Guo et al, 2012).…”

Section: Hasil Dan Pembahasan Produksi Susu Dan Pertumbuhan Bobot Badan Anak Tikusunclassified

Efek Ekstrak Basah Daun Plectranthus amboinicus (L.) Terhadap Produksi Susu, Konsumsi Pakan, Pertumbuhan Bobot Badan Tikus dan Anakan

Wibisono¹,

Banten²,

Dianti³

et al. 2021

Peningkatan Produktivitas Pertanian Era Society 5.0 Pasca Pandemi

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Insufisiensi laktasi menjadi masalah kesehatan masyarakat karena meningkatkan risiko kesakitan dan kematian bayi. Plectranthus amboinicus (L.) telah dilaporkan efeknya pada peningkatan produksi susu. Dalam beberapa tahun terakhir, metode ekstraksi basah menggunakan pelarut air belum dilaporkan pengaruhnya terhadap produksi susu. Penelitian ini bertujuan untuk 1) mendapatkan dosis ekstrak basah yang efektif terhadap produksi susu, konsumsi pakan, pertumbuhan berat badan tikus dan anakan, 2) memperoleh waktu optimum dalam meningkatkan produksi susu, konsumsi pakan, pertumbuhan berat badan tikus dan anakan, dan 3) mengetahui hubungan produksi susu, konsumsi pakan, pertumbuhan berat badan tikus dan anakan. Penelitian dilakukan di Tropical Biopharmaca Research Center, IPB University, pada Februari 2019 hingga Agustus 2019. Lima belas ekor tikus betina (Rattus norvegicus) galur Sprague-Dawley (SD) dibagi menjadi tiga kelompok. Kelompok pertama sebagai kontrol, kelompok kedua adalah tikus yang diberi dosis 162 mg/kgBB (dosis 1), dan kelompok ketiga adalah tikus yang diberi dosis 324 mg/kgBB (dosis 2). Hasil penelitian menunjukkan bahwa dosis 324 mg/kgBB dapat meningkatkan produksi susu tikus (2,81 ± 0,47 g), pertumbuhan bobot badan anak tikus (18,43 ± 0,21 g), dan konsumsi pakan tikus (18,86 ± 0,18 g) dibandingkan dengan kontrol (masing-masing 1,79 ± 0,89 g, 13,39 ± 0,29 g, 13,14 ± 0,33 g). Pertumbuhan bobot badan tikus pada dosis 2 mengalami penurunan 1,79 ± 0,94 g dibandingkan dengan kelompok kontrol 0,43 ± 0,49 g. Waktu optimal untuk peningkatan produksi susu diperoleh pada hari ke 9-16. Peningkatan produksi susu berkorelasi positif dengan pertumbuhan bobot badan anak tikus dan konsumsi pakan tikus. Di sisi lain, produksi susu berkorelasi negatif dengan pertumbuhan berat badan tikus.

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“…Furthermore, the extraction of flavonoid compounds from Tagetes patula generated optimal results using acetone-water (Munhoz et al, 2014). Flavonoids from torbangun leaf are best extracted using ethanol-hexane as a solvent (Wibisono et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Optimization Extraction for Total Phenolic, Flavonoid Contents, and Antioxidant Activity with Different Solvents and UPLC-MS/MS Metabolite Profiling of Justicia gendarussa Burm.f

Marliani¹,

Artika²,

Nurcholis³

2022

CMUJNS

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Justicia gendarussa Burm.f contains polyphenol, which has pharmacological activity. Therefore, this study aims to show the potential of antioxidant compounds in J. gendarussa. The extraction method used was an experimental design that maximized phenolic recovery with the highest antioxidant activity from the leaves of this plant. Furthermore, a simplex-centroid design was used to evaluate the various extractor solvents (water, ethanol, and hexane) and their binary or ternary mixtures, while UPLC-MS/MS analysis was used to identify bioactive compounds. The total phenol (TPC) and flavonoid content (TFC) of the optimized extracts were determined using Follin-Ciocalteu and colorimetric methods, as well as their antioxidant activity were determined using the 2,2-diphenyl-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The results was optimized to a value of 0.844 using water-hexane solvents with a predicted TPC, TFC, DPPH, and FRAP of 112.076 mg GAE/g, 34.926 mg QE/g, 45.477 µmol TE/g, and 353.611 µmol TE/g, respectively. Additionally, the UPLC-MS/MS analysis showed 62 compounds such as phenolics, isoflavonoids, alkaloids, terpenoids, alcohols, and other groups of components. This study shows the potential of antioxidant compounds J. gendarussa. Keywords: Extraction optimization, Justicia gendarussa Burm.f, Simplex centroid design, Polyphenols, Antioxidant

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Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design

Cited by 10 publications

References 27 publications

Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes

Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes

Efek Ekstrak Basah Daun Plectranthus amboinicus (L.) Terhadap Produksi Susu, Konsumsi Pakan, Pertumbuhan Bobot Badan Tikus dan Anakan

Optimization Extraction for Total Phenolic, Flavonoid Contents, and Antioxidant Activity with Different Solvents and UPLC-MS/MS Metabolite Profiling of Justicia gendarussa Burm.f

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