Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes

Abstract

Abstract. Sahid ZD, Syukur M, Maharijaya A, Nurcholis W. 2021. Polyphenol content and pharmacological activities of Capsicum frutescens and C. chinense genotypes. Biodiversitas 22: 3838-3843. Chili is a horticultural crop with potential as a functional food crop. This is because chili has beneficial biochemical parameters such as antioxidants, phenolics, flavonoids, and ?-glucosidase inhibitors. This study evaluated the biochemical parameters in twelve genotypes of two chili species (Capsicum frutescens and Capsicum chinense). Information from this research can be used as a reference in chili plant breeding activities which are directed as functional food plants. The method of measuring biochemical parameters using an ELISA reader with a modified method. Samples were measured with a microplate and repeated three times. The results that emerged from the ELISA reader were then converted with Microsoft Excel and analyzed using the R program. The results showed that the Capsicum chinense species (Habanero Fransisca) had the highest ?-glucosidase inhibitory activity (52.52%) compared to other genotypes of the Capsicum frutescens species. The highest TPC (19.42), TFC (2.51), and antioxidant FRAP (117.27) were shown by the pure line genotype F8 285290-290-2-2-4-4-1. The biochemical content of the chili genotypes tested was divided into three major groups. This grouping is not affected based on the species used. The correlation between antioxidants using the FRAP method with TFC (0.69) and TPC (0.83) is positive and significantly different. In conclusion, information on biochemical content can be used as a basis for developing genotypes, especially for the purposes of plant breeding activities in the future.

Total phenolics, flavonoids, antioxidant activity, and α-glucosidase inhibitory activity of ornamental pepper and several other lines

“…Each replication used ripe chilies from twenty chili plants for biochemical analysis. Biochemical analysis used microplate reader ELISA method (Sahid et al, 2021). ANOVA, DMRT 5% test, and Pearson correlation analysis were performed using the SAS 9.0.…”

The Analysis of Morphological Diversity and Polyphenols Content of Celosia cristata in M2 Population Induced by Ethyl Methane Sulphonate

“…The procedure for analyzing antioxidant activity using the DPPH method in consonance with Suleria et al [17] with a slight modification, absorbance was determined at a wavelength of 515 nm. The antioxidant analysis using the FRAP method following Sahid et al [18] with a slight modification determined absorbance at a wavelength of 593 nm. The instrument used to measure the absorbance of the test sample was a nano-spectrophotometer (SPECTROstarNano BMG LAB-TECH).…”

Effect of combined NPK fertilizer on polyphenol contents and antioxidant activity in methanol extract of Curcuma xanthorhiza

“…TFC was estimated using the AlCl 3 -colorimetric method, referred to Sahid et al [13] 10 µL methanol extract of C. xanthorrhiza was mixed with 60 µL methanol, 10 µL AlCl 3 ( 10%), 10 µL CH 3 COOK (1M), and 110 µL distilled water. The absorbance of the mixed solution was measured using an ELISA reader (Epoch Biotek, USA) at a wavelength of 415 nm.…”

“…Polyphenolic compounds are broadly distributed in all parts of the plant. Various studies have noted the pharmacological effects of polyphenols as anticancer, anti-inflammatory, anti-androgen, and anti-diabetic [12,13]. The antioxidant capacity is one of the essential pharmacological properties of polyphenolic compounds [14][15][16].…”