Optimization of the Enzymatic Saccharification Process of Empty Fruit Bunch Pretreated with Laccase Enzyme

Abstract

The saccharification of laccase-pretreated empty fruit bunch (EFB) was optimized in a lab-scale experiment using one-factor-at-a-time (OFAT) and response surface methodology (RSM). After pretreatment, the degree of delignification was checked by noting the weight loss (%) after pretreatment, and also by the quantity of total sugar produced after saccharification with cellulase enzyme. OFAT studies of saccharification of the pretreated EFB showed that the biomass was best saccharified using cellulase enzyme at the following conditions: enzyme concentration of 30 IU/g of EFB, substrate concentration of 5.0% w/v, 50 °C, saccharification time of 24 h, and pH 5. This combination exhibited the highest yield of total sugar (28% w/w). Although 29% w/w yield was achieved with an enzyme concentration of 40 IU/g of EFB, this increase in yield was not proportional to the increased enzyme concentration and, therefore, was considered insignificant. Statistical analysis of the combined effects of pH and temperature showed that pH had a more significant effect than the temperature on the saccharification process, based on a P < 0.05 significance level. The effect of pH on total sugar production was more significant than the temperature in both linear and quadratic functions. In sum, the saccharification of laccase-pretreated EFB should follow the optimized process conditions achieved in the current study.

Optimization of saccharification conditions of black rice (cv. Poireton) using microbial strains through response surface methodology

“…The results of a confirmation experiment under the optimum conditions agreed well with model predictions. Shah et al also found enzymatic saccharification through RSM of laccase‐pretreated empty fruit bunch in a laboratory‐scale experiment using one‐factor‐at‐a‐time analysis . The results of these various experiments using RSM are in line with our present study.…”

Acid–Base Pretreatment and Enzymatic Hydrolysis of Palm Oil Mill Effluent in a Single Reactor System for Production of Fermentable Sugars

“…On the other hand, at a speed of more than 150 rpm, the amount of sugar yield was maximized [28]. Shah et al [31] described that moderate agitation was operated to increase the rate of enzymatic hydrolysis, and maximum yield was found at 150 rpm. Several researchers recommended that the optimum agitation for maximum enzymatic hydrolysis of rice straw was approximately 100 rpm [28] found at 180 rpm.…”

Optimization of Saccharification of Biological Pre-Treated Rice Straw by Response Surface Methodology

“…This could be explained on the basis of gain of kinetic energy by the reactant molecules with increase in temperature and hence a more productive collision per unit time, resulting in better yields at high temperature (Segel, 1976). Shah et al, (2016) optimized the conditions for saccharification of laccase pretreated empty fruit bunch by RSM at enzyme concentration, 30 FPU/g; substrate concentration, 5.0% (w/v); temperature, 50° C; saccharification time, 24 h and pH 5, resulting in highest total sugars yield of 28.0% (w/w). The optimum parameters obtained for the hydrolysis of rice straw with cellulase from Aspergillus niger were pH 6.0; substrate concentration, 12% (w/v) and enzyme concentration, 10 U/g of rice straw.…”