Optimization of RT–PCR for the Detection of <i>Bean leaf roll virus</i> in Plant Hosts and Insect Vectors

Ortiz, Vilma; Castro, S.; Romero, Javier

doi:10.1111/j.1439-0434.2004.00929.x

Cited by 24 publications

(9 citation statements)

References 25 publications

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“…One μL of total RNA template was used for each RT-PCR reaction. Bean leaf roll virus was amplified with the specific primers BLRV F Ortiz and BLRV R Ortiz and thermocycling conditions as described by Oritz et al (2005), yielding a 385 bp product. Beet western yellows virus was amplified with specific primers BWYF and BWYV PCR and thermocycling conditions as described by Miranda et al (1995), yielding a 428 bp product.…”

Section: Pcrmentioning

confidence: 99%

Effective management of viruses in pulse crops in south eastern Australia should include management of weeds

Freeman¹,

Aftab²

2011

Australasian Plant Pathol.

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This study addressed the role of weeds in the epidemiology of the six most important pulse viruses in the production areas of south eastern Australia-Alfalfa mosaic virus-Bean yellow mosaic virus-cucumber mosaic virus-pea seedborne mosaic virus-bean leafroll virus-beet western yellows virus-pulse viruses-legume viruses-weeds. We found that the weed host range of these viruses was greater than previously reported and that not only were weeds a potential source of virus during the cropping season, but many persisted over hot, dry summers providing a "green bridge" for the viruses and their aphid vectors between cropping seasons. Weed surveys were conducted for the six pulse viruses in and around pulse crops during the 2000-2001 growing seasons. Virus was detected in 55 of 180 weed samples (selected as representative of 1416 samples), which included 15 species in 12 genera of seven families, including six allegedly new host-virus records. A weed survey was conducted between pulse growing seasons, over the summer of 2002 to determine the survival of weed hosts. It was found that even under severe drought conditions most weed species survived in a range of locations, providing a potential reservoir for viruses and allowing aphid vectors to over-summer on green hosts. Inoculation of 36 weed species common to the area resulted in additional potential weed hosts. As a result, weed control would seem to be an important element in controlling viruses in pulse crops in south eastern Australia.

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Section: Pcrmentioning

confidence: 99%

Effective management of viruses in pulse crops in south eastern Australia should include management of weeds

Freeman¹,

Aftab²

2011

Australasian Plant Pathol.

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“…Many studies for field survey of CABYV incidence showed enzyme linked immunosorbent assay (ELISA) using antibody specific to CABYV was effective for the CABYV detection method ( Abou-Jawdah et al, 2000 ; Deng et al, 1997 ; Kassem et al, 2007 ; Mnari-Hattab et al, 2005 ). In recent reports, ELISA is not sensitive enough to detect the presence of CABYV and other luteoviruses in plants ( Figueira et al, 1997 ; Oritz et al, 2005 ; Van den Heuvel et al, 1989 ). We set up the molecular detection method, RT-PCR, for diagnosis of CABYV in this study and showed the RT-PCR analysis is highly reliable to detect CABYV in suspicious samples.…”

Section: Discussionmentioning

confidence: 99%

Biological and Molecular Characterization of a Korean Isolate of Cucurbit aphidborne yellows virus Infecting Cucumis Species in Korea

Choi¹,

Yoon²,

Choi

2015

The Plant Pathology Journal

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Surveys of yellowing viruses in plastic tunnels and in open field crops of melon (Cucumis melo cultivar catalupo), oriental melon (C. melo cultivar oriental melon), and cucumber (C. sativus) were carried out in two melon-growing areas in 2014, Korea. Severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. The symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thickening of older leaves. RT-PCR analysis using total RNA extracted from diseased leaves did not show any synthesized products for four cucurbit-infecting viruses; Beet pseudo-yellows virus, Cucumber mosaic virus, Cucurbit yellows stunting disorder virus, and Melon necrotic spot virus. Virus identification using RT-PCR showed Cucurbit aphid-borne yellows Virus (CABYV) was largely distributed in melon, oriental melon and cucumber. This result was verified by aphid (Aphis gossypii) transmission of CABYV. The complete coat protein (CP) gene amplified from melon was cloned and sequenced. The CP gene nucleotide and the deduced amino acid sequence comparisons as well as phylogenetic tree analysis of CABYV CPs showed that the CABYV isolates were undivided into subgroups. Although the low incidence of CABYV in infections to cucurbit crops in this survey, CABYV may become an important treat for cucurbit crops in many different regions in Korea, suggesting that CABYV should be taken into account in disease control of cucurbit crops in Korea.

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“…Le MWMV n'a pas été détecté au niveau des 175 échantillons de cucurbitacées collectés bien que ce virus est transmis par le puceron Aphis gossypii très présent en Tunisie (Mnari-Hattab & Cherif, 1992; Ben Halima-Kamel & Ben Hamouda, 1993) et dont l'efficacité de transmission de ce virus est de 31 à 56%, (Quiot-Douine et al ., 1990). Ainsi, l'absence de ce virus dans les échantillons testés pourrait être due soit à son absence réelle en Tunisie, soit à la faible sensibilité des tests sérologiques qui sont 10 4 moins sensibles que les tests moléculaires ou qui ne détectent pas certains serotypes éloignés (van den Heuvel & Peters, 1989;Figueira et al ., 1997;Ortiz et al ., 2005). Le MWMV a été détecté au Maroc sur melon et courgette (Fischer & Lockhart, 1974), et au Soudan en 1994 sur C. melo var.…”

Section: Discussionunclassified

Identification et distribution des virus responsables de mosaiques chez les cucurbitacées en Tunisie

Mnari‐Hattab¹,

Jebari

Zouba³

2008

EPPO Bulletin

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Les cucurbitacées sont économiquement importantes en Tunisie, elles sont sujettes à plusieurs maladies virales qui ont des effets néfastes sur les rendements et la valeur marchande de la production. Des symptômes de mosaïques sévères sur les feuilles ont été observés dans toutes les cultures de cucurbitacées de plein champ et sous abris. Des prospections (2000–2001, 2002–2003) ont été réalisées afin de tenter d’identifier les virus responsables de ces symptômes. Les échantillons symptomatiques ont été collectés dans les régions majeures de production des cucurbitacées et testés par DAS‐ELISA pour la présence des virus suivants: ZYMV, WMV, MWMV, PRSV‐W, ZYFV CMV, SqMV et MNSV. Cette étude a confirmé la présence de six virus (WMV, CMV, PRSV‐W, ZYMV, ZYFV et SqMV) déjà décrits avec une évolution très remarquable de la fréquence du ZYMV dans toutes les régions prospectées et sur toutes les espèces testées. Le pourcentage d’infection a atteint les 34% durant les prospections (2002–2003). Par contre le MWMV s’est révélé absent au niveau de tous les échantillons testés. Par ailleurs, nous avons mis en évidence pour la première fois la présence du MNSV dans plus de 9% des échantillons testés et ce sur melon de plein champ et sous abris, sur courge et sur pastèque. En outre, nous rapportons pour la première fois l’infection d’une cucurbitacée sauvage, Ecballium elaterium, par le CMV, PRSV‐W et WMV. Les résultats ont montré aussi qu’un pourcentage d’échantillons montrant des symptômes de mosaïques n’a pas été expliqué par la présence de l’un ou l’autre des virus étudiés. Ceci pourrait s’expliquer par l’existence d’autres virus ou de facteurs abiotiques qui restent à identifier.

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Optimization of RT–PCR for the Detection of Bean leaf roll virus in Plant Hosts and Insect Vectors

Cited by 24 publications

References 25 publications

Effective management of viruses in pulse crops in south eastern Australia should include management of weeds

Effective management of viruses in pulse crops in south eastern Australia should include management of weeds

Biological and Molecular Characterization of a Korean Isolate of Cucurbit aphidborne yellows virus Infecting Cucumis Species in Korea

Identification et distribution des virus responsables de mosaiques chez les cucurbitacées en Tunisie

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