Optimization of rhizogenesis for in vitro shoot culture of Pinus massoniana Lamb.

Wang, Yin; Yao, Ruiling

doi:10.1007/s11676-019-01076-8

Cited by 11 publications

(6 citation statements)

References 25 publications

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“…Similarly, the recent study of in vitro rooting from Pterocarpus marsupium also found that beyond the optimum dose of IBA induced undesirable callus at the basal end of microshoot, and no rooting was observed (Ahmad et al 2020). Otherwise, for applied PGRs in vitro rooting, there are some different results, supplementation with 1.2 uM NAA in the medium was effective for rooting induction in P. massoniana (Wang and Yao 2019b), most of the clones from Pinus densiflora rooted on medium supplemented with 0.2 mg • L −1 NAA (Zhu et al 2019). IBA was also reported to be better than with NAA or a mixture of both auxins in vitro rooting (Zhu et al 2007), in contrast to this, IBA (2 mg • L −1 ) application with NAA (0.1 mg • L −1 ) was a preferable alternative than IBA only for the Ficus religiosa root induction, subsequent development of the induced roots usually proceeded (Hesami and Daneshvar 2019).…”

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 87%

“…As we all know, many coniferous species have a low capacity for rooting in vitro, and the most common problem encountered in the micropropagation of pines is adventitious root formation, and also because of this, reducing the possibilities of applying these techniques for large scale micropropagation (Bonga 2017). Generally, auxins play an essential role in regulating adventitious root formation, and IBA or NAA are often used to promote in vitro rooting (Kalia et al 2007;Alvarez et al 2009;Wang and Yao 2019a;Wang and Yao 2019b). After two months of cultivation on rootpromoting medium, the highest rooting percentages were < 30% in our present study.…”

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 99%

“…Our results indicated that although a small part of the plants was rooted in the tested medium combinations, root formation was occasionally observed and rarely. Compared with Pinus peuce (Stojičić et al 2012), Pinus densiflora (Zhu et al 2019), and P. massoniana (Wang and Yao 2019b), korean pine may be a difficult coniferous species for in vitro rooting in micropropagation. Therefore, further studies on in vitro rooting are necessary.…”

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 99%

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Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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The de novo organogenesis system for Korean pine of this economically and ecologically coniferous species was successfully established using sprouted seeds as the original explants. After 30 days of incubation, 92.67% of explants produced direct shoots on Gupta and Durzan(DCR) medium containing 2 mg • L −1 kinetin (KT) in combination 0.5 mg • L −1 thidiazuron (TDZ) with a maximum of about 15 shoots per explant respectively. We also confirmed the organogenic regeneration pattern by scanning electron microscopy (SEM) observation. For shoot elongation and growth after 60 days of culture, we obtained the highest mean length of 34.99 mm from DCR basal media supplemented with 6-benzyladenine (6-BA; 0.2 mg • L −1 ), 1-Naphthaleneacetic acid (NAA; 0.1 mg • L −1 ), and activated charcoal (AC; 1 g • L −1 ). The highest rooting percentages of 20.74-21.48% were observed within two months in the 1/2 DCR medium (major elements halved) enriched with 0.05 mg•L −1 NAA and 0.5 or 1 mg • L −1 indole-3-butyric acid (IBA).Rooted plants showed a survival rate of 90.28% in perlite: peat: vermiculite = 1:1:1 after acclimatization. This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and these data will be helpful to the clonal propagation and conservation of Korean pine.

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Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 87%

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 99%

Section: Rooting and Acclimatization Of Pinus Koraiensis Microshootsmentioning

confidence: 99%

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Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang

Bai

et al. 2021

Plant Cell Tiss Organ Cult

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“…The formation of roots in shoots generated in vitro has been reported in other species such as Pinus massoniana, through the addition of ANA to the culture medium (Wang and Yao, 2019), although a good part of species of the genus Pinus generate a low or null number of roots (Thorpe, 2004). The tests performed with three concentrations of ANA added to the DCR medium showed this response with Pinus patula (Table 1), and therefore in the micro-and mini-grafting tests the seedlings that formed a low number of roots were considered as negative control, and on the other hand, the data of seedlings derived from the in vitro germination as positive control.…”

Section: Resultsmentioning

confidence: 79%

Mini grafts: alternative to obtain complete plants of Pinus patula propagated in vitro

Castillo-Martínez

Cruz

Upton

2022

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Micro-grafting and mini-grafting trials were carried out in order to obtain complete Pinus patula plants generated in vitro, derived from the fact that the formation of roots in micro-propagated shoots is very low or null. On the other hand, there is the possibility of generating clones with outstanding genetic characteristics. P. patula seeds were established in aseptic conditions and geminated in vitro in DCR medium; the aerial part was dissected from the base and roots were kept in DCR medium added with 0.5 mg l-1 of NAA to maintain them and served as mini rootstock, the shoots were transferred to DCR medium added with 2.0 mg l-1 of BA to form seedlings with an average of 3.9 shoots per explant at 30 days. On the other hand, 2-month-old nursery seedlings were used as rootstock for the mini-grafts. For the micro-graft tests, the shoots obtained were taken and the apical meristems with approximately 5 mm were isolated to conduct micro-grafting at the base with roots, and in the case of mini-grafts the shoots generated were allowed to grow to a length of 3 cm on average and grafted onto nursery seedlings. In both cases, the achievement of the grafted materials and the length were evaluated to measure the growth of the materials that had positive success. The response of micro-grafts was very low, 10% success, in addition to the manipulation of the meristems being very complex, which generated oxidation in the tissues. On the contrary, the mini-grafts showed 93.3 of grafting success and average growth of 26.05 cm, two months after the grafting process

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“…NAA, an auxin analogue, is extensively applied as a rooting medium for several plants species, such as Arabidopsis thaliana (Chen et al, 2014) and Pinus massoniana Lamb. (Wang;Yao, 2019).…”

Section: Embryogenic Callus Induction and Multiplication Of Leaf And mentioning

confidence: 99%

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

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Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.

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Optimization of rhizogenesis for in vitro shoot culture of Pinus massoniana Lamb.

Abstract: of mature P. massoniana trees by optimizing explant types (rejuvenated), subculture times (20 subcultures, 35-40 days per subculture) and addition of NAA + PBZ to the rooting medium. The conditions can be used for efficient plantlet regeneration of P. massoniana.

Cited by 11 publications

References 25 publications

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Mini grafts: alternative to obtain complete plants of Pinus patula propagated in vitro

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

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