Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Liang, Yan; Bai, Xue; Xu, Xiaoyong; Wang, Jing; Pan, Peng

doi:10.1007/s11240-021-02164-6

Cited by 5 publications

(3 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the initial stage of shoot induction, a heavy bunch of shoot buds had formed. Similar results were also observed in Populus deltoides (Yadav et al, 2009), Melia azedarach (Vila et al, 2005), Saccharum officinarum (Sathish et al, 2018), and Pinus koraiensis (Liang et al, 2022).…”

Section: Discussionsupporting

confidence: 82%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Casuarina equisetifolia is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic in vitro propagation protocol in C. equisetifolia using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, in vitro derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old in vitro derived plant of C. equisetifolia for synthetic seed production, and it was reported that CaCl2 · 2H2O (100 mM) + Na2-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of C. equisetifolia. It will also help in meeting the growing demands of C. equisetifolia in the forest industry.

show abstract

Section: Discussionsupporting

confidence: 82%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Korean pine ( Pinus koraiensis ) is utilized for its wood and is also an important tree species of cold–temperate needle-leaved latitudinal forests [ 1 ]. Additionally, it is utilized for its medicinal properties, and its nuts are used in a variety of foods [ 2 ]. There are a low number of natural stands of Korean pine, and most plantations are relatively young (most of them have just started to set and have not entered the fruiting stage), making it difficult to meet the growing demand for its natural products [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Hormone Signal Transduction, Carbohydrate Metabolism, Heat Shock Proteins, and SCF Complexes before and after Fertilization of Korean Pine Ovules

Liu

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

The fertilization process is a critical step in plant reproduction. However, the mechanism of action and mode of regulation of the fertilization process in gymnosperms remain unclear. In this study, we investigated the molecular regulatory networks involved in the fertilization process in Korean pine ovules through anatomical observation, physiological and biochemical assays, and transcriptome sequencing technology. The morphological and physiological results indicated that fertilization proceeds through the demise of the proteinaceous vacuole, egg cell division, and pollen tube elongation. Auxin, cytokinin, soluble sugar, and soluble starch contents begin to decline upon fertilization. Transcriptomic data analysis revealed a large number of differentially expressed genes at different times before and after fertilization. These genes were primarily involved in pathways associated with plant hormone signal transduction, protein processing in the endoplasmic reticulum, fructose metabolism, and mannose metabolism. The expression levels of several key genes were further confirmed by qRT-PCR. These findings represent an important step towards understanding the mechanisms underlying morphological changes in the Korean pine ovule during fertilization, and the physiological and transcriptional analyses lay a foundation for in-depth studies of the molecular regulatory network of the Korean pine fertilization process.

show abstract

“…Owing to overexploitation, the species' natural stands are fragile and diminishing, and its plantations are being extended: for instance, Korean pine is the most important plantation tree species in northeast China [3]. Breeding, selection, and seedling production, as well as pine tree growth and development, have been receiving marked research attention [2,4,[6][7][8] due to its high productivity and aesthetics, with the main aim of introducing it into other areas, different from their natural habitats.…”

Section: Introductionmentioning

confidence: 99%

Cambisol Mycobiome in a Long-Term Field Experiment with Korean Pine as a Sole Edificator: A Case Study

Naumova

Кузнецова

Alikina

et al. 2022

Applied Microbiology

View full text Add to dashboard Cite

A culture-independent mycobiome survey in Haplic Cambisol under Korean pine in a long-term field experiment in the Russian Far East was conducted using sequence analysis of the ITS region amplified with ITS3/ITS4 primers using the metagenomic DNA as a matrix. Overall 758 fungal OTUs were identified, representing 15 phyla, 47 classes, 104 orders, 183 families, and 258 genera. More OTUs represented the Ascomycota phylum (513) than Basidiomycota (113), with both phyla together comprising 95% of the relative abundance. The Leotiomycetes class was ultimately prevailing; apparently contributing significantly to the organic matter decomposition and microbial biomass in soil, as shown by a PCA. Only two dominant OTUs (Pseudogymnoascus sp. and Hyaloscyphaceae, both Ascomycota) were common in the studied samples. The presented high mycobiome diversity in soil under the monospecies artificial forest, where Korean pine had been the sole edificator for forty years, allows concluding that plant chemistry diversity is the main factor shaping the soil mycobiome in such an environment. The obtained data provide a reference for further studies of soil mycobiota, especially under Korean pine with its aesthetic, as well as nut-producing, potential. The results can be helpful in the targeted creating of a soil mycobiome beneficial for pines in afforestation and remediation contexts.

show abstract

Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

Cited by 5 publications

References 63 publications

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Transcriptomic Analysis of Hormone Signal Transduction, Carbohydrate Metabolism, Heat Shock Proteins, and SCF Complexes before and after Fertilization of Korean Pine Ovules

Cambisol Mycobiome in a Long-Term Field Experiment with Korean Pine as a Sole Edificator: A Case Study

Contact Info

Product

Resources

About