Optimization of resolution in micellar electrokinetic chromatography

Rasmussen, Henrik T.; Goebel, Lisa K.; McNair, Harold M.

doi:10.1002/jhrc.1240140107

Cited by 45 publications

(12 citation statements)

References 24 publications

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“…Polymeric surfactants have also been reported to provide unique chemical selectivity [7,8]. Additionally, mixed micelles of SDS and Brij 35 can provide significantly different selectivity from SDS micelles alone [13].…”

Section: Poor Selectivitymentioning

confidence: 98%

Method development for Micellar Electrokinetic Chromatography

Palmer

2003

Chromatographia

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SummaryMicellar Electrokinetic Chromatography is useful technique for the separation of a variety of analytes. Because it is somewhat different in theory and application than conventional forms of chromatography, method development can present a challenge. A rational and systematic approach to method development is presented in this report, following the basic sequence of a tutorial presented at the recent International Symposium on Chromatography in Leipzig. This approach follows a flow chart scheme and recognition of common migration patterns to suggest the path to an optimum separation.

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Section: Poor Selectivitymentioning

confidence: 98%

Method development for Micellar Electrokinetic Chromatography

Palmer

2003

Chromatographia

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“…As a consequence of this, the electrophoretic mobility of the mixed micelle decreases which results in a decrease of the tmc (e.g. the separation window becomes smaller) [13]. Ultimately at very large Brij concentrations, the electrophoretic mobility of the mixed micelle will approach that of the electroosmotic mobility (to).…”

Section: Effect Of Brijmentioning

confidence: 99%

“…The addition of the non-ionic surfactant Brij to an ionic surfactant in MEKC has found to bring about changes in the migration window and selectivity [13]. It is assumed that the changes are the result of the formation of mixed micelles.…”

Section: Migration Behaviour Of Fatty Acids With Sdbs-brij Mixturesmentioning

confidence: 99%

Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

Erim

Kraak

1995

Journal of Chromatography A

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Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids Erim, F.B.; Xu, X.; Kraak, J.C. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractA micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C8-C2o fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.

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“…Derivatization is usually performed off-line or using online condition [6][7][8][9][10]. The latter approach is more efficient in that on-line derivatization is less time consuming, reduces the consumption of both sample and derivatizing reagents, and can be easily automated [9].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, in-column derivatization applies mild conditions (ca. 50ЊC) by injecting samples right after derivatizing reagents and thus can reduce undesired by-products [7,9,10]. Meanwhile, both methods in a conventional splitless mode usually impose restrictions on injected sample volume (<5 L) [11].…”

Section: Introductionmentioning

confidence: 99%

Sample stacking with in‐column silylation for less volatile polar compounds using GC‐MS

Cho

2012

J of Separation Science

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This paper presents sample stacking with in-column silylation (SIS) for quantitative analysis of less volatile polar compounds using gas chromatography-mass spectrometry (GC-MS). This was achieved by the combination of sandwiched in-column silylation and multiple injections (up to 100 times or 100 μL in total). N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used as a silylating reagent. For the SIS technique, samples were introduced multiple N times (N = 2~100) into a capillary column in between BSTFA injections. The quantitative characteristic of SIS technique was studied using bisphenol A (BPA) as a model compound. The sandwiched in-column silylation for the less volatile polar compounds effectively replaced polar hydrogen with trimethylsilyl group to reduce sample adsorption and band broadening. Meanwhile, multiple injections at the SIS technique contributed to increase the sensitivity quantitatively. The capability and limitation of this analytical approach were further investigated with various types of compounds such as hydroxyls, carboxylic acids, and amine.

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Optimization of resolution in micellar electrokinetic chromatography

Abstract: Summarywhere lo is the elution time of a component distributing exclusively in the mobile phase and t, , the elution time of a component distributing exclusively into the micellar phase to and t,,, may be related to vo and v, , as

Cited by 45 publications

References 24 publications

Method development for Micellar Electrokinetic Chromatography

Method development for Micellar Electrokinetic Chromatography

Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

Sample stacking with in‐column silylation for less volatile polar compounds using GC‐MS

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