Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids

Abstract

Application of micellar electrokinetic chromatography and indirect UV detection for the analysis of fatty acids Erim, F.B.; Xu, X.; Kraak, J.C. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractA micellar electrokinetic chromatographic system using indirect UV detection was developed for the analysis of saturated fatty acids. Sodium dodecyl benzenesulfonate (SDBS) was used as micellar surfactant as well as chromophore for the indirect detection. The effects of the addition of acetonitrile and Brij on the migration and solubility of the fatty acids was investigated. With a mixture of 10 mM SDBS + 50% acetonitrile + 30 mM Brij, a complete separation of the C8-C2o fatty acids was obtained. The applicability of the developed system for the analysis of fatty acids in butter is demonstrated.

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications

“…Instead of a CnTAB, Brij, which is a polyethylene glycol dodecylether, can be used in mixed micelles with SDS or maybe, even better, with SC. These mixed micelles probably have similar selectivity properties to CnTAB micelles [59]; compare also [128,156,162,2671. The concentration of Brij should not exceed approximately 5 mmoVL for neutral analytes; otherwise, the micelles lose too much charge, leading to a small elution window (compare Diagram 9).…”

Design and synthesis of a hydrophilic fluorescent derivatization reagent for carboxylic acids, 4‐N‐(4‐N‐aminoethyl)piperazino‐7‐nitro‐2,1,3‐benzoxadiazole (NBD‐PZ‐NH2), and its application to capillary electrophoresis with laser‐induced fluorescence detection

“…There have been several reports for the separation and detection of fatty acids by CE (Collet and Gareil, 1997;Koval et al, 1985;RoldanAssad and Gareil, 1995;Salimi-Moosavi and Cassidy, 1996;Schmitz and Gab, 1997;Drange and Lundanes, 1997;Erim et al, 1995;Wang et al, 1998;Wan et al, 1999). Most of the methods utilize the indirect UV absorbance detection system by adding p-methoxybenzoate (Collet and Gareil, 1997;Roldan-Assad and Gareil, 1995), p-toluenesulfonate (Salmi-Moosavi and Cassidy, 1996), anthraquinone-2-sulfonic acid (Drange and Lundanes, 1997), or dodecyl benzenesulfonate (Erim et al, 1995) as a chromophore, or utilize indirect fluorescence detection system by adding fluorescein (Wang et al, 1998) in the migration medium. However, these methods lack the sensitivity and the detection limits are usually at the 10 À5 M level.…”

Method development for the analysis of trans‐fatty acids in hydrogenated oils by capillary electrophoresis

“…In general, the use of alkaline buffers with chromophoric anionic species or chromophoric additives for the indirect UV detection of fatty acids is the preferred mode of analysis. Additionally, to improve the solubility and discrimination of equivalent chain length fatty acids, the use of cyclodextrins, surfactants and organic solvents, have all been attempted successfully [17][18][19]. However, to our knowledge, there are no reports on the separation of long-chain trans-fatty acids by capillary electrophoresis so far.…”